##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AvrII BamHI BclI
BfrI BlnI BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
Bst1107I BstBI BstZ17I ClaI DraI
Eco47III EcoRV EcoT22I FbaI HindIII
HpaI KpnI MamI MluI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
ScaI SfuI SgrAI SpeI SphI
SspBI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 3344 GACGTC GACGT^C
AccI 437 GTCGAC GT^[AC][GT]AC
AccIII 729 TCCGGA T^CCGGA
Asp700I 91 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 488 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4311 TGCGCA TGC^GCA
BbrPI 3273 CACGTG CAC^GTG
BcgI 384 CGAGGCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1175 GGATAC GTATCC(6/5)
BseAI 729 TCCGGA T^CCGGA
BspEI 729 TCCGGA T^CCGGA
BsrDI 4225 CATTGC GCAATG(2/0)
BssHII 1444 GCGCGC G^CGCGC
BstXI 3078 CCATGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 3876 CGGACCG CG^G[AT]CCG
DraIII 699 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1759 GAATTC G^AATTC
FspI 4311 TGCGCA TGC^GCA
HincII 437 GTCGAC GT[CT]^[GA]AC
HindII 437 GTCGAC GT[CT]^[GA]AC
KspI 854 CCGCGG CCGC^GG
MfeI 1463 CAATTG C^AATTG
MroI 729 TCCGGA T^CCGGA
MseI 4332 TTAA T^TAA
MunI 1463 CAATTG C^AATTG
PflFI 488 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3273 CACGTG CAC^GTG
PmlI 3273 CACGTG CAC^GTG
RsrII 3876 CGGACCG CG^G[AT]CCG
SacII 854 CCGCGG CCGC^GG
SalI 437 GTCGAC G^TCGAC
SexAI 700 ACCAGGT A^CC[AT]GGT
SfiI 2461 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 5212 TACGTA TAC^GTA
Tru9I 4332 TTAA T^TAA
Tth111I 488 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 91 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 1724 AAATTT [GA]^AATT[CT]
1759 GAATTC
AhdI 2156 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2512 GACTGCACGTC
AocI 1225 CCTGAGG CC^T[ACGT]AGG
4685 CCTGAGG
ApoI 1724 AAATTT [GA]^AATT[CT]
1759 GAATTC
AspEI 2156 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2512 GACTGCACGTC
BsaAI 3273 CACGTG [CT]AC^GT[GA]
5212 TACGTA
BsaWI 729 TCCGGA [AT]^CCGG[AT]
2283 TCCGGT
BstEII 1135 GGTCACC G^GT[ACGT]ACC
3938 GGTAACC
BstPI 1135 GGTCACC G^GT[ACGT]ACC
3938 GGTAACC
Bsu36I 1225 CCTGAGG CC^T[ACGT]AGG
4685 CCTGAGG
DrdI 1127 GACCTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1142 GACATCATCGTC
Eam1105I 2156 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2512 GACTGCACGTC
Eco81I 1225 CCTGAGG CC^T[ACGT]AGG
4685 CCTGAGG
EcoO65I 1135 GGTCACC G^GT[ACGT]ACC
3938 GGTAACC
FseI 258 GGCCGGCC GGCCGG^CC
4471 GGCCGGCC
MslI 157 CATGTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3506 CATGAGCGTG
NcoI 2799 CCATGG C^CATGG
3078 CCATGG
SapI 4007 GAAGAGC GCTCTTC(1/4)
142 GCTCTTC
SbfI 3517 CCTGCAGG CCTGCA^GG
4069 CCTGCAGG
Sse8387I 3517 CCTGCAGG CCTGCA^GG
4069 CCTGCAGG
StuI 1014 AGGCCT AGG^CCT
5277 AGGCCT
TfiI 3802 GAATC G^A[AT]TC
5193 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI