##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII BamHI
BbrPI BsaAI BsiWI BspDI BspMI
BsrDI BssSI BstEII BstPI Bsu36I
ClaI CpoI DrdI Eco81I EcoO65I
EcoT22I FseI FspI HindIII HpaI
KpnI MluI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SgrAI SnaBI SphI Sse8387I
SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflIII 4869 ACATGT A^C[GA][CT]GT
AhdI 157 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2857 AGCGCT AGC^GCT
ApaI 1059 GGGCCC GGGCC^C
AspEI 157 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1128 GGCGCC GGCGC^C
BciVI 3137 GGATAC GTATCC(6/5)
BclI 4574 TGATCA T^GATCA
BsmBI 3877 GAGACG CGTCTC(1/5)
Bsp1407I 3109 TGTACA T^GTACA
BspLU11I 4869 ACATGT A^CATGT
BsrFI 953 GCCGGC [GA]^CCGG[CT]
BsrGI 3109 TGTACA T^GTACA
BssHII 1742 GCGCGC G^CGCGC
Bst1107I 3175 GTATAC GTA^TAC
BstBI 4634 TTCGAA TT^CGAA
BstZ17I 3175 GTATAC GTA^TAC
Cfr10I 953 GCCGGC [GA]^CCGG[CT]
DraIII 725 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 157 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2857 AGCGCT AGC^GCT
EcoRI 4704 GAATTC G^AATTC
FbaI 4574 TGATCA T^GATCA
KasI 1128 GGCGCC G^GCGCC
KspI 2166 CCGCGG CCGC^GG
MfeI 224 CAATTG C^AATTG
MunI 224 CAATTG C^AATTG
NaeI 953 GCCGGC GCC^GGC
NarI 1128 GGCGCC GG^CGCC
NdeI 2615 CATATG CA^TATG
NgoMIV 953 GCCGGC G^CCGGC
NspI 4869 ACATGT [GA]CATG^[CT]
NspV 4634 TTCGAA TT^CGAA
PflMI 4287 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1499 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2166 CCGCGG CCGC^GG
SapI 3800 GAAGAGC GCTCTTC(1/4)
SfoI 1128 GGCGCC GGC^GCC
SfuI 4634 TTCGAA TT^CGAA
SmaI 1228 CCCGGG CCC^GGG
SpeI 2011 ACTAGT A^CTAGT
SspBI 3109 TGTACA T^GTACA
StuI 1036 AGGCCT AGG^CCT
Van91I 4287 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1228 CCCGGG C^CCGGG
XmaI 1228 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 3175 GTATAC GT^[AC][GT]AC
3347 GTCTAC
AflII 1402 CTTAAG C^TTAAG
1910 CTTAAG
AlwNI 3360 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
4288 CAGGTACTG
AvaI 1228 CCCGGG C^[CT]CG[GA]G
3155 CCCGAG
AvrII 1179 CCTAGG C^CTAGG
4938 CCTAGG
BfrI 1402 CTTAAG C^TTAAG
1910 CTTAAG
BglII 2669 AGATCT A^GATCT
4615 AGATCT
BlnI 1179 CCTAGG C^CTAGG
4938 CCTAGG
BlpI 1548 GCTTAGC GC^T[ACGT]AGC
3475 GCTGAGC
Bpu1102I 1548 GCTTAGC GC^T[ACGT]AGC
3475 GCTGAGC
BsaBI 2664 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4569 GATCTTGATC
BsiEI 913 CGGCCG CG[GA][CT]^CG
955 CGGCCG
BsmI 2577 GAATGC GAATGC(1/-1)
5004 GAATGC
BsoBI 1228 CCCGGG C^[CT]CG[GA]G
3155 CCCGAG
BspHI 2658 TCATGA T^CATGA
3256 TCATGA
BsrBI 1106 GAGCGG CCGCTC(-3/-3)
3478 GAGCGG
CelII 1548 GCTTAGC GC^T[ACGT]AGC
3475 GCTGAGC
EagI 913 CGGCCG C^GGCCG
955 CGGCCG
EclXI 913 CGGCCG C^GGCCG
955 CGGCCG
Eco52I 913 CGGCCG C^GGCCG
955 CGGCCG
EcoNI 851 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3729 CCTGGGACAGG
EcoRV 4510 GATATC GAT^ATC
4655 GATATC
HaeII 1128 GGCGCC [GA]GCGC^[CT]
2857 AGCGCT
HincII 2767 GTTGAC GT[CT]^[GA]AC
4519 GTCAAC
HindII 2767 GTTGAC GT[CT]^[GA]AC
4519 GTCAAC
MamI 2664 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4569 GATCTTGATC
PstI 470 CTGCAG CTGCA^G
573 CTGCAG
RcaI 2658 TCATGA T^CATGA
3256 TCATGA
SacI 881 GAGCTC GAGCT^C
4916 GAGCTC
SspI 2391 AATATT AAT^ATT
4790 AATATT
XbaI 3604 TCTAGA T^CTAGA
4692 TCTAGA
XcmI 2201 CCATCCCCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4395 CCACCCCCAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspEI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NheI NlaIII
NlaIV PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII