##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AhdI AscI Asp700I Asp718I
AspEI AspI BciVI BclI BfrI
BglII BsaBI BseAI BsiWI Bsp1407I
BspEI BspLU11I BsrGI Bst1107I BstBI
BstXI BstZ17I DraI DrdI Eam1105I
EcoRV EcoT22I FbaI HpaI KpnI
MamI MluI MroI NotI NsiI
NspV PacI PflFI PmeI Psp1406I
PvuI ScaI SfuI SnaBI SpeI
SspBI SspI SwaI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 617 GGCGCC G[GA]^CG[CT]C
AgeI 3159 ACCGGT A^CCGGT
AviII 1419 TGCGCA TGC^GCA
BamHI 2524 GGATCC G^GATCC
BbeI 617 GGCGCC GGCGC^C
BbrPI 1484 CACGTG CAC^GTG
BcgI 2161 GCAAAGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1484 CACGTG [CT]AC^GT[GA]
BsaHI 617 GGCGCC G[GA]^CG[CT]C
BsaI 4649 GAGACC GGTCTC(1/5)
BsaWI 3159 ACCGGT [AT]^CCGG[AT]
BspDI 4607 ATCGAT AT^CGAT
BspHI 5362 TCATGA T^CATGA
BssSI 2630 CACGAG CACGAG(-5/-1)
ClaI 4607 ATCGAT AT^CGAT
CpoI 3059 CGGACCG CG^G[AT]CCG
FseI 4377 GGCCGGCC GGCCGG^CC
FspI 1419 TGCGCA TGC^GCA
Hin1I 617 GGCGCC G[GA]^CG[CT]C
HindIII 2658 AAGCTT A^AGCTT
KasI 617 GGCGCC G^GCGCC
MfeI 3395 CAATTG C^AATTG
MunI 3395 CAATTG C^AATTG
NarI 617 GGCGCC GG^CGCC
NdeI 3414 CATATG CA^TATG
NheI 1922 GCTAGC G^CTAGC
NruI 655 TCGCGA TCG^CGA
PinAI 3159 ACCGGT A^CCGGT
PmaCI 1484 CACGTG CAC^GTG
PmlI 1484 CACGTG CAC^GTG
RcaI 5362 TCATGA T^CATGA
RsrII 3059 CGGACCG CG^G[AT]CCG
SacI 5366 GAGCTC GAGCT^C
SalI 4185 GTCGAC G^TCGAC
SapI 3213 GCTCTTC GCTCTTC(1/4)
SbfI 1624 CCTGCAGG CCTGCA^GG
SfoI 617 GGCGCC GGC^GCC
Sse8387I 1624 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 3804 GAATTC [GA]^AATT[CT]
5413 GAATTC
AocI 462 CCTGAGG CC^T[ACGT]AGG
1389 CCTAAGG
Aor51HI 2926 AGCGCT AGC^GCT
3522 AGCGCT
ApoI 3804 GAATTC [GA]^AATT[CT]
5413 GAATTC
AseI 3383 ATTAAT AT^TAAT
5130 ATTAAT
AsnI 3383 ATTAAT AT^TAAT
5130 ATTAAT
AvrII 4030 CCTAGG C^CTAGG
4217 CCTAGG
BlnI 4030 CCTAGG C^CTAGG
4217 CCTAGG
BlpI 988 GCTCAGC GC^T[ACGT]AGC
1894 GCTTAGC
Bpu1102I 988 GCTCAGC GC^T[ACGT]AGC
1894 GCTTAGC
BssHII 635 GCGCGC G^CGCGC
4818 GCGCGC
Bsu36I 462 CCTGAGG CC^T[ACGT]AGG
1389 CCTAAGG
CelII 988 GCTCAGC GC^T[ACGT]AGC
1894 GCTTAGC
DraIII 900 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2304 CACGCTGTG
Eco47III 2926 AGCGCT AGC^GCT
3522 AGCGCT
Eco81I 462 CCTGAGG CC^T[ACGT]AGG
1389 CCTAAGG
EcoNI 341 CCTGACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4572 CCTCCCACAGG
EcoRI 3804 GAATTC G^AATTC
5413 GAATTC
HincII 3972 GTCAAC GT[CT]^[GA]AC
4185 GTCGAC
HindII 3972 GTCAAC GT[CT]^[GA]AC
4185 GTCGAC
NspI 3165 GCATGC [GA]CATG^[CT]
4866 GCATGC
PaeR7I 1977 CTCGAG C^TCGAG
4154 CTCGAG
PshBI 3383 ATTAAT AT^TAAT
5130 ATTAAT
SexAI 2975 ACCTGGT A^CC[AT]GGT
3248 ACCTGGT
SfiI 366 GGCCCCTCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
589 GGCCGCCCGGGCC
SgrAI 1040 CGCCGGTG C[GA]^CCGG[CT]G
3158 CACCGGTG
SphI 3165 GCATGC GCATG^C
4866 GCATGC
XhoI 1977 CTCGAG C^TCGAG
4154 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PshAI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI