##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI AscI Asp718I
AspEI AviII AvrII BbeI BbrPI
BciVI BglII BlnI BlpI Bpu1102I
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BsrFI BssHII Bst1107I BstBI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I FseI FspI HpaI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
Aor51HI 3995 AGCGCT AGC^GCT
ApaI 1797 GGGCCC GGGCC^C
AspI 3942 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 3206 GGCACC G^G[CT][GA]CC
BanII 1797 GGGCCC G[GA]GC[CT]^C
BcgI 3166 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 32 GCCACGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 4882 CACGTA [CT]AC^GT[GA]
BsaBI 357 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 817 ACCGGA [AT]^CCGG[AT]
BstAPI 1778 GCACCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III 3995 AGCGCT AGC^GCT
MamI 357 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3482 CAATTG C^AATTG
MunI 3482 CAATTG C^AATTG
NcoI 1910 CCATGG C^CATGG
PflFI 3942 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 2547 AGTACT AGT^ACT
SpeI 5351 ACTAGT A^CTAGT
SphI 4109 GCATGC GCATG^C
Tth111I 3942 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 353 TCTAGA T^CTAGA
XcmI 109 CCACGTCAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 91 GACGCC G[GA]^CG[CT]C
218 GACGCC
AflII 2645 CTTAAG C^TTAAG
5531 CTTAAG
Alw44I 1439 GTGCAC G^TGCAC
1878 GTGCAC
ApaLI 1439 GTGCAC G^TGCAC
1878 GTGCAC
AseI 2090 ATTAAT AT^TAAT
5155 ATTAAT
AsnI 2090 ATTAAT AT^TAAT
5155 ATTAAT
AspHI 1439 GTGCAC G[AT]GC[AT]^C
1878 GTGCAC
BamHI 1086 GGATCC G^GATCC
5555 GGATCC
BbsI 428 GAAGAC GAAGAC(2/6)
5275 GTCTTC
BbvCI 2703 GCTGAGG CCTCAGC(-5/-2)
3847 GCTGAGG
BclI 2892 TGATCA T^GATCA
4495 TGATCA
BfrI 2645 CTTAAG C^TTAAG
5531 CTTAAG
BpuAI 428 GAAGAC GAAGAC(2/6)
5275 GTCTTC
BsaHI 91 GACGCC G[GA]^CG[CT]C
218 GACGCC
BseRI 2345 GAGGAG GAGGAG(10/8)
2925 GAGGAG
BsiHKAI 1439 GTGCAC G[AT]GC[AT]^C
1878 GTGCAC
BsmBI 3947 CGTCTC CGTCTC(1/5)
4070 CGTCTC
BsmI 5412 GAATGC GAATGC(1/-1)
618 GCATTC
Bsp1407I 2070 TGTACA T^GTACA
3573 TGTACA
BspHI 845 TCATGA T^CATGA
4710 TCATGA
BspLU11I 3763 ACATGT A^CATGT
4559 ACATGT
BsrGI 2070 TGTACA T^GTACA
3573 TGTACA
BssSI 4031 CACGAG CACGAG(-5/-1)
4147 CACGAG
BstEII 1865 GGTGACC G^GT[ACGT]ACC
1948 GGTGACC
BstPI 1865 GGTGACC G^GT[ACGT]ACC
1948 GGTGACC
DsaI 1910 CCATGG C^C[GA][CT]GG
3406 CCGTGG
EcoO65I 1865 GGTGACC G^GT[ACGT]ACC
1948 GGTGACC
EcoRI 1495 GAATTC G^AATTC
2486 GAATTC
EcoRV 2478 GATATC GAT^ATC
5380 GATATC
EcoT22I 5071 ATGCAT ATGCA^T
5396 ATGCAT
FbaI 2892 TGATCA T^GATCA
4495 TGATCA
HaeII 18 AGCGCC [GA]GCGC^[CT]
3995 AGCGCT
Hin1I 91 GACGCC G[GA]^CG[CT]C
218 GACGCC
HincII 3416 GTTGAC GT[CT]^[GA]AC
3494 GTCAAC
HindII 3416 GTTGAC GT[CT]^[GA]AC
3494 GTCAAC
HindIII 1588 AAGCTT A^AGCTT
3365 AAGCTT
NdeI 680 CATATG CA^TATG
928 CATATG
NsiI 5071 ATGCAT ATGCA^T
5396 ATGCAT
PflMI 3502 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4125 CCAGCTACTGG
PshAI 106 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
130 GACCGCTGTC
PshBI 2090 ATTAAT AT^TAAT
5155 ATTAAT
PstI 467 CTGCAG CTGCA^G
832 CTGCAG
PvuII 1594 CAGCTG CAG^CTG
5210 CAGCTG
RcaI 845 TCATGA T^CATGA
4710 TCATGA
SspBI 2070 TGTACA T^GTACA
3573 TGTACA
SspI 3471 AATATT AAT^ATT
5484 AATATT
Van91I 3502 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4125 CCAGCTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI Asp700I
AvaI AvaII BalI BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI XmnI