##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AflIII AscI
AseI AsnI Asp700I AviII BamHI
BbeI BcgI BclI BglII BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstYI BstZ17I ClaI CpoI
DraI EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII KasI KspI
MamI MfeI MflI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NotI NsiI NspI NspV PacI
PaeR7I PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SfoI
SfuI SnaBI SpeI SphI SspBI
SspI SwaI TfiI XbaI XhoI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 1066 GAATTT [GA]^AATT[CT]
AflII 4570 CTTAAG C^TTAAG
Aor51HI 4272 AGCGCT AGC^GCT
ApoI 1066 GAATTT [GA]^AATT[CT]
AvrII 5283 CCTAGG C^CTAGG
BbrPI 5739 CACGTG CAC^GTG
BbsI 2921 GTCTTC GAAGAC(2/6)
BfrI 4570 CTTAAG C^TTAAG
BlnI 5283 CCTAGG C^CTAGG
BpuAI 2921 GTCTTC GAAGAC(2/6)
BsaAI 5739 CACGTG [CT]AC^GT[GA]
BsiEI 5098 CGGCCG CG[GA][CT]^CG
DrdI 3704 GACGCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5098 CGGCCG C^GGCCG
EclXI 5098 CGGCCG C^GGCCG
Eco47III 4272 AGCGCT AGC^GCT
Eco52I 5098 CGGCCG C^GGCCG
EcoNI 4823 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 2305 GTTAAC GT[CT]^[GA]AC
HindII 2305 GTTAAC GT[CT]^[GA]AC
HpaI 2305 GTTAAC GTT^AAC
NruI 1710 TCGCGA TCG^CGA
PmaCI 5739 CACGTG CAC^GTG
PmlI 5739 CACGTG CAC^GTG
SbfI 4058 CCTGCAGG CCTGCA^GG
ScaI 4599 AGTACT AGT^ACT
SexAI 12 ACCTGGT A^CC[AT]GGT
Sse8387I 4058 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 2882 GACGCC G[GA]^CG[CT]C
5681 GACGCC
Alw44I 3941 GTGCAC G^TGCAC
5352 GTGCAC
AlwI 4136 GGATC GGATC(4/5)
4933 GGATC
ApaLI 3941 GTGCAC G^TGCAC
5352 GTGCAC
BciVI 901 GGATAC GTATCC(6/5)
2624 GTATCC
BsaHI 2882 GACGCC G[GA]^CG[CT]C
5681 GACGCC
BsmBI 5165 GAGACG CGTCTC(1/5)
5679 GAGACG
BsrDI 3380 GCAATG GCAATG(2/0)
6264 GCAATG
EarI 4439 CTCTTC CTCTTC(1/4)
6098 GAAGAG
Hin1I 2882 GACGCC G[GA]^CG[CT]C
5681 GACGCC
Ksp632I 4439 CTCTTC CTCTTC(1/4)
6098 GAAGAG
NcoI 171 CCATGG C^CATGG
5494 CCATGG
PflMI 3114 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5129 CCAGGTCGTGG
PpuMI 3526 GGGTCCC [GA]G^G[AT]CC[CT]
5010 GGGTCCT
SacI 3821 GAGCTC GAGCT^C
3863 GAGCTC
SapI 6098 GAAGAGC GCTCTTC(1/4)
4438 GCTCTTC
SgrAI 1153 CACCGGTG C[GA]^CCGG[CT]G
2044 CACCGGTG
SmaI 3467 CCCGGG CCC^GGG
4663 CCCGGG
TaqI 215 TCGA T^CGA
5839 TCGA
TthHB8I 215 TCGA T^CGA
5839 TCGA
Van91I 3114 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5129 CCAGGTCGTGG
XmaCI 3467 CCCGGG C^CCGGG
4663 CCCGGG
XmaI 3467 CCCGGG C^CCGGG
4663 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
Acc65I AccI AccII AciI AfaI
AgeI AhdI AluI AlwNI AocI
ApaI Asp718I AspEI AspHI AspI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eam1105I Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NheI
NlaIII NlaIV PflFI PinAI PleI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SmlI StuI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I XcmI