##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AclI AcsI AflII ApoI
AscI AseI AsnI Asp700I Asp718I
BcgI BciVI BfrI BsaBI BsiWI
BspDI BspHI Bst1107I BstBI BstZ17I
ClaI CpoI DraI EcoRI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MunI NdeI
NheI NotI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AatII 2837 GACGTC GACGT^C
AccI 3545 GTCTAC GT^[AC][GT]AC
AgeI 2081 ACCGGT A^CCGGT
AhdI 7220 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 7220 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3026 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 685 CCTAGG C^CTAGG
BamHI 6129 GGATCC G^GATCC
BclI 7239 TGATCA T^GATCA
BlnI 685 CCTAGG C^CTAGG
BspLU11I 2103 ACATGT A^CATGT
DrdI 2531 GACCAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 7220 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 190 ATGCAT ATGCA^T
FbaI 7239 TGATCA T^GATCA
MseI 3657 TTAA T^TAA
NruI 7699 TCGCGA TCG^CGA
NsiI 190 ATGCAT ATGCA^T
PflFI 3026 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2081 ACCGGT A^CCGGT
PshAI 6265 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Tru9I 3657 TTAA T^TAA
Tsp509I 2973 AATT ^AATT
Tth111I 3026 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AccIII 1689 TCCGGA T^CCGGA
6501 TCCGGA
BseAI 1689 TCCGGA T^CCGGA
6501 TCCGGA
BsmI 2442 GCATTC GAATGC(1/-1)
6866 GCATTC
BspEI 1689 TCCGGA T^CCGGA
6501 TCCGGA
EcoRV 5789 GATATC GAT^ATC
6539 GATATC
FseI 1045 GGCCGGCC GGCCGG^CC
7580 GGCCGGCC
MroI 1689 TCCGGA T^CCGGA
6501 TCCGGA
SfiI 865 GGCCGCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
7120 GGCCGAGAGGGCC
SphI 1584 GCATGC GCATG^C
5454 GCATGC
TfiI 6590 GAATC G^A[AT]TC
7142 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 166 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AspHI AvaI
AvaII AviII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I SbfI
ScrFI SexAI SfaNI SfcI SfoI
SmaI SmlI Sse8387I SspBI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI