##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AccIII      AclI        AgeI        AhdI        Aor51HI     
AscI        AspEI       BbeI        BciVI       BglII       
BlpI        Bpu1102I    BsaBI       BsaWI       BseAI       
BsiWI       BsmBI       BspDI       BspEI       BsrBI       
BsrDI       BsrFI       BssHII      Bst1107I    BstEII      
BstPI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DrdI        Eam1105I    Eco47III    EcoO65I     
EcoRV       FseI        HgaI        HindIII     KasI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        PacI        PaeR7I      PinAI       
PmeI        PshAI       Psp1406I    RsrII       SalI        
SbfI        SfoI        SgrAI       SpeI        Sse8387I    
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII         689  GACGTC               GACGT^C
Acc65I        572  GGTACC               G^GTACC
AccI         5915  GTAGAC               GT^[AC][GT]AC
AcyI          689  GACGTC               G[GA]^CG[CT]C
AflII        4132  CTTAAG               C^TTAAG
Alw44I       3542  GTGCAC               G^TGCAC
ApaLI        3542  GTGCAC               G^TGCAC
Asp718I       572  GGTACC               G^GTACC
AvaI          241  CCCGGG               C^[CT]CG[GA]G
BbrPI         301  CACGTG               CAC^GTG
BcgI         4729  CGATTCTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         4132  CTTAAG               C^TTAAG
BglI          253  GCCGATCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         689  GACGTC               G[GA]^CG[CT]C
BsoBI         241  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     3806  TGTACA               T^GTACA
BsrGI        3806  TGTACA               T^GTACA
EagI          251  CGGCCG               C^GGCCG
EclXI         251  CGGCCG               C^GGCCG
Eco52I        251  CGGCCG               C^GGCCG
Hin1I         689  GACGTC               G[GA]^CG[CT]C
HpaI         3139  GTTAAC               GTT^AAC
KpnI          572  GGTACC               GGTAC^C
KspI          106  CCGCGG               CCGC^GG
NcoI         3326  CCATGG               C^CATGG
PmaCI         301  CACGTG               CAC^GTG
PmlI          301  CACGTG               CAC^GTG
PvuI          255  CGATCG               CGAT^CG
SacII         106  CCGCGG               CCGC^GG
SapI         1698  GCTCTTC              GCTCTTC(1/4)
SexAI        1376  ACCAGGT              A^CC[AT]GGT
SfiI          252  GGCCGATCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          241  CCCGGG               CCC^GGG
SnaBI         971  TACGTA               TAC^GTA
SspBI        3806  TGTACA               T^GTACA
XcmI         3097  CCAAGAGGGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         241  CCCGGG               C^CCGGG
XmaI          241  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII         107  CGCG                 CG^CG
              210  CGCG                
AflIII        498  ACATGT               A^C[GA][CT]GT
             5175  ACATGT              
AlwNI         598  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             5720  CAGCTTCTG           
ApaI         5425  GGGCCC               GGGCC^C
             5967  GGGCCC              
AspI         3418  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3588  GACAGGGTC           
AviII         116  TGCGCA               TGC^GCA
              269  TGCGCA              
BclI          953  TGATCA               T^GATCA
             7048  TGATCA              
BsiEI         251  CGGCCG               CG[GA][CT]^CG
              255  CGATCG              
BspLU11I      498  ACATGT               A^CATGT
             5175  ACATGT              
BssSI        1457  CACGAG               CACGAG(-5/-1)
             2900  CTCGTG              
BstBI        1055  TTCGAA               TT^CGAA
             1447  TTCGAA              
BstUI         107  CGCG                 CG^CG
              210  CGCG                
DraIII       5399  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             6999  CACTTAGTG           
DsaI          106  CCGCGG               C^C[GA][CT]GG
             3326  CCATGG              
EcoRI         763  GAATTC               G^AATTC
             3483  GAATTC              
EcoT22I      1513  ATGCAT               ATGCA^T
             4212  ATGCAT              
FbaI          953  TGATCA               T^GATCA
             7048  TGATCA              
FspI          116  TGCGCA               TGC^GCA
              269  TGCGCA              
HaeII         229  AGCGCC               [GA]GCGC^[CT]
             5547  AGCGCC              
MvnI          107  CGCG                 CG^CG
              210  CGCG                
NsiI         1513  ATGCAT               ATGCA^T
             4212  ATGCAT              
NspV         1055  TTCGAA               TT^CGAA
             1447  TTCGAA              
PflFI        3418  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3588  GACAGGGTC           
PpuMI        2168  AGGTCCC              [GA]G^G[AT]CC[CT]
             5158  AGGACCC             
ScaI          868  AGTACT               AGT^ACT
             3724  AGTACT              
SfuI         1055  TTCGAA               TT^CGAA
             1447  TTCGAA              
SphI         1344  GCATGC               GCATG^C
             6053  GCATGC              
Tth111I      3418  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3588  GACAGGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AocI        ApoI        AseI        AsnI        Asp700I     
AspHI       AvaII       AvrII       BalI        BamHI       
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BlnI        BmyI        BpmI        
BpuAI       BsaAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspHI       BspMI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PshBI       PstI        
PvuII       RcaI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XbaI        XhoII       XmnI