##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AatII AclI AflII AgeI BcgI
BclI BfrI BglII BsaBI Bsp1407I
BspDI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoRV FbaI HpaI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PinAI PmeI
PshAI Psp1406I PvuI RsrII SalI
SfuI SnaBI SpeI SspBI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 4253 GGTACC G^GTACC
AhdI 3701 GACTTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 3143 GGCGCGCC GG^CGCGCC
AseI 7236 ATTAAT AT^TAAT
AsnI 7236 ATTAAT AT^TAAT
Asp700I 2390 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4253 GGTACC G^GTACC
AspEI 3701 GACTTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1333 TGCGCA TGC^GCA
BbeI 456 GGCGCC GGCGC^C
BbrPI 7018 CACGTG CAC^GTG
BciVI 1778 GGATAC GTATCC(6/5)
BsiEI 45 CGACCG CG[GA][CT]^CG
BsiWI 148 CGTACG C^GTACG
BsmI 4024 GCATTC GAATGC(1/-1)
BssHII 3144 GCGCGC G^CGCGC
BstEII 1454 GGTCACC G^GT[ACGT]ACC
BstPI 1454 GGTCACC G^GT[ACGT]ACC
Eam1105I 3701 GACTTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4826 CCTCCCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1454 GGTCACC G^GT[ACGT]ACC
EcoRI 1842 GAATTC G^AATTC
EcoT22I 336 ATGCAT ATGCA^T
FseI 1168 GGCCGGCC GGCCGG^CC
FspI 1333 TGCGCA TGC^GCA
HindIII 4757 AAGCTT A^AGCTT
KasI 456 GGCGCC G^GCGCC
KpnI 4253 GGTACC GGTAC^C
NarI 456 GGCGCC GG^CGCC
NsiI 336 ATGCAT ATGCA^T
PaeR7I 6523 CTCGAG C^TCGAG
PmaCI 7018 CACGTG CAC^GTG
PmlI 7018 CACGTG CAC^GTG
PshBI 7236 ATTAAT AT^TAAT
SapI 180 GAAGAGC GCTCTTC(1/4)
ScaI 1990 AGTACT AGT^ACT
SfoI 456 GGCGCC GGC^GCC
SgrAI 2376 CACCGGCG C[GA]^CCGG[CT]G
XhoI 6523 CTCGAG C^TCGAG
XmnI 2390 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Aor51HI 4554 AGCGCT AGC^GCT
5286 AGCGCT
AvrII 4831 CCTAGG C^CTAGG
6502 CCTAGG
BlnI 4831 CCTAGG C^CTAGG
6502 CCTAGG
BlpI 5192 GCTCAGC GC^T[ACGT]AGC
5410 GCTCAGC
Bpu1102I 5192 GCTCAGC GC^T[ACGT]AGC
5410 GCTCAGC
BspHI 4180 TCATGA T^CATGA
4665 TCATGA
BspLU11I 1294 ACATGT A^CATGT
5715 ACATGT
BsrDI 3221 GCAATG GCAATG(2/0)
4928 GCAATG
CelII 5192 GCTCAGC GC^T[ACGT]AGC
5410 GCTCAGC
DraI 3631 TTTAAA TTT^AAA
6771 TTTAAA
DraIII 1518 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2448 CACCCAGTG
Eco47III 4554 AGCGCT AGC^GCT
5286 AGCGCT
HincII 444 GTTGAC GT[CT]^[GA]AC
1860 GTCAAC
HindII 444 GTTGAC GT[CT]^[GA]AC
1860 GTCAAC
RcaI 4180 TCATGA T^CATGA
4665 TCATGA
SbfI 1410 CCTGCAGG CCTGCA^GG
5239 CCTGCAGG
SexAI 277 ACCAGGT A^CC[AT]GGT
5010 ACCTGGT
SmaI 556 CCCGGG CCC^GGG
4985 CCCGGG
Sse8387I 1410 CCTGCAGG CCTGCA^GG
5239 CCTGCAGG
SspI 7420 AATATT AAT^ATT
7485 AATATT
XmaCI 556 CCCGGG C^CCGGG
4985 CCCGGG
XmaI 556 CCCGGG C^CCGGG
4985 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII