##################################
# NO CUT ENZYMES (   37 enzymes) #
##################################

Acc65I      AflII       AgeI        AhdI        AscI        
Asp718I     AspEI       BfrI        BsiWI       BsrBI       
BsrDI       Bst1107I    BstBI       BstZ17I     CpoI        
DrdI        Eam1105I    FseI        KpnI        NheI        
NotI        NruI        NspV        PacI        PinAI       
PmeI        PshAI       PvuI        RsrII       SalI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        SwaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AclI          596  AACGTT               AA^CGTT
Aor51HI      4722  AGCGCT               AGC^GCT
AseI         2332  ATTAAT               AT^TAAT
AsnI         2332  ATTAAT               AT^TAAT
AvrII        3542  CCTAGG               C^CTAGG
BamHI        4433  GGATCC               G^GATCC
BbeI         2302  GGCGCC               GGCGC^C
BbvCI        7252  GCTGAGG              CCTCAGC(-5/-2)
BciVI        4019  GGATAC               GTATCC(6/5)
BclI          816  TGATCA               T^GATCA
BglII        1858  AGATCT               A^GATCT
BlnI         3542  CCTAGG               C^CTAGG
BsaBI        4226  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI         778  ATCGAT               AT^CGAT
BspHI        6702  TCATGA               T^CATGA
BssHII       3766  GCGCGC               G^CGCGC
BstEII       3151  GGTGACC              G^GT[ACGT]ACC
BstPI        3151  GGTGACC              G^GT[ACGT]ACC
ClaI          778  ATCGAT               AT^CGAT
DraIII       4950  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     4722  AGCGCT               AGC^GCT
EcoO65I      3151  GGTGACC              G^GT[ACGT]ACC
EcoRI        3356  GAATTC               G^AATTC
EcoRV         521  GATATC               GAT^ATC
FbaI          816  TGATCA               T^GATCA
HpaI         5406  GTTAAC               GTT^AAC
KasI         2302  GGCGCC               G^GCGCC
KspI         1986  CCGCGG               CCGC^GG
MamI         4226  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2370  CAATTG               C^AATTG
MunI         2370  CAATTG               C^AATTG
NarI         2302  GGCGCC               GG^CGCC
PflMI        4949  CCACGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        2332  ATTAAT               AT^TAAT
Psp1406I      596  AACGTT               AA^CGTT
RcaI         6702  TCATGA               T^CATGA
SacII        1986  CCGCGG               CCGC^GG
SbfI         7154  CCTGCAGG             CCTGCA^GG
ScaI         2556  AGTACT               AGT^ACT
SfoI         2302  GGCGCC               GGC^GCC
SmaI         7379  CCCGGG               CCC^GGG
Sse8387I     7154  CCTGCAGG             CCTGCA^GG
Van91I       4949  CCACGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         4803  TCTAGA               T^CTAGA
XmaCI        7379  CCCGGG               C^CCGGG
XmaI         7379  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI         1208  GTCTAC               GT^[AC][GT]AC
             1370  GTAGAC              
AccIII       2144  TCCGGA               T^CCGGA
             3988  TCCGGA              
Asp700I       784  GAATGCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              922  GAATCTGTTC          
AviII        3329  TGCGCA               TGC^GCA
             7144  TGCGCA              
BcgI         2185  CGACGGAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1637  GCAGATTCTTCG        
BfaI         3543  CTAG                 C^TAG
             4804  CTAG                
BlpI         2092  GCTGAGC              GC^T[ACGT]AGC
             7498  GCTCAGC             
BpmI         2036  CTGGAG               CTGGAG(16/14)
             4914  CTGGAG              
Bpu1102I     2092  GCTGAGC              GC^T[ACGT]AGC
             7498  GCTCAGC             
BsaWI        2144  TCCGGA               [AT]^CCGG[AT]
             3988  TCCGGA              
BseAI        2144  TCCGGA               T^CCGGA
             3988  TCCGGA              
BspEI        2144  TCCGGA               T^CCGGA
             3988  TCCGGA              
CelII        2092  GCTGAGC              GC^T[ACGT]AGC
             7498  GCTCAGC             
EcoT22I      6537  ATGCAT               ATGCA^T
             6746  ATGCAT              
FspI         3329  TGCGCA               TGC^GCA
             7144  TGCGCA              
HindIII      2960  AAGCTT               A^AGCTT
             5539  AAGCTT              
MaeI         3543  CTAG                 C^TAG
             4804  CTAG                
MluI         6664  ACGCGT               A^CGCGT
             7562  ACGCGT              
MroI         2144  TCCGGA               T^CCGGA
             3988  TCCGGA              
NcoI         3866  CCATGG               C^CATGG
             3952  CCATGG              
NdeI         6493  CATATG               CA^TATG
             6690  CATATG              
NsiI         6537  ATGCAT               ATGCA^T
             6746  ATGCAT              
PaeR7I       2217  CTCGAG               C^TCGAG
             3655  CTCGAG              
SspI         4812  AATATT               AAT^ATT
             5069  AATATT              
XhoI         2217  CTCGAG               C^TCGAG
             3655  CTCGAG              
XmnI          784  GAATGCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              922  GAATCTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  149 enzymes) #
#########################################

AatII       AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        Alw44I      AlwI        
AlwNI       AocI        ApaI        ApaLI       ApoI        
AspHI       AspI        AvaI        AvaII       BalI        
BanI        BanII       BbrPI       BbsI        BbvI        
BcnI        BglI        BmyI        BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspLU11I    BspMI       BsrFI       BsrGI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PflFI       PleI        
PmaCI       PmlI        PpuMI       PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SphI        
SspBI       StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     XcmI        XhoII