##################################
# NO CUT ENZYMES ( 37 enzymes) #
##################################
AflII AscI AseI AsnI BcgI
BfrI BglII BsiWI BspDI BspHI
BstBI ClaI DraI EagI EclXI
Eco52I EcoNI FseI MluI NheI
NotI NruI NspV PacI PmeI
PshBI PvuI RcaI SalI SfiI
SfuI SnaBI SpeI SphI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 5901 GACGTC GACGT^C
AclI 7866 AACGTT AA^CGTT
AgeI 3896 ACCGGT A^CCGGT
AocI 2818 CCTCAGG CC^T[ACGT]AGG
AspI 4862 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1618 GGATCC G^GATCC
BclI 4540 TGATCA T^GATCA
BsiEI 1430 CGACCG CG[GA][CT]^CG
BstEII 2621 GGTCACC G^GT[ACGT]ACC
BstPI 2621 GGTCACC G^GT[ACGT]ACC
Bsu36I 2818 CCTCAGG CC^T[ACGT]AGG
CpoI 3726 CGGTCCG CG^G[AT]CCG
DraIII 3157 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2818 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2621 GGTCACC G^GT[ACGT]ACC
EcoRI 6615 GAATTC G^AATTC
EcoRV 4941 GATATC GAT^ATC
EcoT22I 5660 ATGCAT ATGCA^T
FbaI 4540 TGATCA T^GATCA
HindIII 6825 AAGCTT A^AGCTT
HpaI 1224 GTTAAC GTT^AAC
KspI 77 CCGCGG CCGC^GG
MfeI 2923 CAATTG C^AATTG
MunI 2923 CAATTG C^AATTG
NdeI 3910 CATATG CA^TATG
NsiI 5660 ATGCAT ATGCA^T
PflFI 4862 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3896 ACCGGT A^CCGGT
Psp1406I 7866 AACGTT AA^CGTT
RsrII 3726 CGGTCCG CG^G[AT]CCG
SacII 77 CCGCGG CCGC^GG
SbfI 2232 CCTGCAGG CCTGCA^GG
SexAI 6673 ACCAGGT A^CC[AT]GGT
SgrAI 3895 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 2232 CCTGCAGG CCTGCA^GG
Tth111I 4862 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 514 TCCGGA T^CCGGA
3729 TCCGGA
AhdI 5673 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5901 GACGTCTAGTC
Aor51HI 2139 AGCGCT AGC^GCT
3054 AGCGCT
ApaI 1771 GGGCCC GGGCC^C
6109 GGGCCC
AspEI 5673 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5901 GACGTCTAGTC
AvrII 3773 CCTAGG C^CTAGG
5112 CCTAGG
BbeI 177 GGCGCC GGCGC^C
1972 GGCGCC
BciVI 2579 GGATAC GTATCC(6/5)
6914 GTATCC
BlnI 3773 CCTAGG C^CTAGG
5112 CCTAGG
BlpI 630 GCTGAGC GC^T[ACGT]AGC
1995 GCTAAGC
Bpu1102I 630 GCTGAGC GC^T[ACGT]AGC
1995 GCTAAGC
BseAI 514 TCCGGA T^CCGGA
3729 TCCGGA
BsmI 1550 GAATGC GAATGC(1/-1)
3710 GAATGC
Bsp1407I 5374 TGTACA T^GTACA
5494 TGTACA
BspEI 514 TCCGGA T^CCGGA
3729 TCCGGA
BspLU11I 243 ACATGT A^CATGT
2761 ACATGT
BsrGI 5374 TGTACA T^GTACA
5494 TGTACA
BssHII 30 GCGCGC G^CGCGC
163 GCGCGC
BssSI 6054 CACGAG CACGAG(-5/-1)
4008 CTCGTG
BstXI 770 CCACTGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2984 CCACCCATTTGG
CelII 630 GCTGAGC GC^T[ACGT]AGC
1995 GCTAAGC
DrdI 3117 GACATCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5192 GACCCGCACGTC
Eam1105I 5673 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5901 GACGTCTAGTC
Eco47III 2139 AGCGCT AGC^GCT
3054 AGCGCT
KasI 177 GGCGCC G^GCGCC
1972 GGCGCC
MroI 514 TCCGGA T^CCGGA
3729 TCCGGA
NarI 177 GGCGCC GG^CGCC
1972 GGCGCC
PaeR7I 1742 CTCGAG C^TCGAG
4088 CTCGAG
SacI 4293 GAGCTC GAGCT^C
6151 GAGCTC
ScaI 2699 AGTACT AGT^ACT
4535 AGTACT
SfoI 177 GGCGCC GGC^GCC
1972 GGCGCC
SspBI 5374 TGTACA T^GTACA
5494 TGTACA
XhoI 1742 CTCGAG C^TCGAG
4088 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI ApaLI ApoI Asp700I
Asp718I AspHI AvaI AvaII AviII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
Bst1107I BstAPI BstF5I BstNI BstUI
BstYI BstZ17I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PshAI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI