##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AclI AgeI AscI AseI AsnI
AspI BbrPI BcgI BsiEI BsiWI
BspDI Bst1107I BstBI BstZ17I ClaI
CpoI EagI EclXI Eco52I EcoT22I
HindIII HpaI KspI MfeI MluI
MunI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI SspI
StuI Tth111I
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 3348 GACGTC GACGT^C
AccI 1941 GTCGAC GT^[AC][GT]AC
AccIII 3021 TCCGGA T^CCGGA
AflII 3596 CTTAAG C^TTAAG
AhdI 2786 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 6591 GTGCAC G^TGCAC
Aor51HI 6640 AGCGCT AGC^GCT
ApaLI 6591 GTGCAC G^TGCAC
Asp700I 1631 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2786 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 6805 GGATCC G^GATCC
BbeI 4914 GGCGCC GGCGC^C
BfrI 3596 CTTAAG C^TTAAG
BglI 4795 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2745 AGATCT A^GATCT
BsaAI 6049 TACGTG [CT]AC^GT[GA]
BsaBI 6415 GATAGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3021 TCCGGA [AT]^CCGG[AT]
BseAI 3021 TCCGGA T^CCGGA
BsmBI 1612 GAGACG CGTCTC(1/5)
Bsp1407I 7252 TGTACA T^GTACA
BspEI 3021 TCCGGA T^CCGGA
BsrGI 7252 TGTACA T^GTACA
BssSI 2499 CACGAG CACGAG(-5/-1)
BstEII 2344 GGTGACC G^GT[ACGT]ACC
BstPI 2344 GGTGACC G^GT[ACGT]ACC
DraIII 5721 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2786 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 6640 AGCGCT AGC^GCT
EcoO65I 2344 GGTGACC G^GT[ACGT]ACC
EcoRI 5174 GAATTC G^AATTC
FseI 6624 GGCCGGCC GGCCGG^CC
KasI 4914 GGCGCC G^GCGCC
MamI 6415 GATAGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3021 TCCGGA T^CCGGA
NarI 4914 GGCGCC GG^CGCC
NcoI 453 CCATGG C^CATGG
PaeR7I 4925 CTCGAG C^TCGAG
PshAI 5795 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2924 GAGCTC GAGCT^C
SalI 1941 GTCGAC G^TCGAC
SapI 707 GAAGAGC GCTCTTC(1/4)
SbfI 2626 CCTGCAGG CCTGCA^GG
SexAI 1712 ACCTGGT A^CC[AT]GGT
SfoI 4914 GGCGCC GGC^GCC
Sse8387I 2626 CCTGCAGG CCTGCA^GG
SspBI 7252 TGTACA T^GTACA
SwaI 7269 ATTTAAAT ATTT^AAAT
XbaI 1537 TCTAGA T^CTAGA
XhoI 4925 CTCGAG C^TCGAG
XmnI 1631 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AflIII 4598 ACATGT A^C[GA][CT]GT
5015 ACATGT
ApaI 3543 GGGCCC GGGCC^C
6195 GGGCCC
AviII 1674 TGCGCA TGC^GCA
2856 TGCGCA
BspLU11I 4598 ACATGT A^CATGT
5015 ACATGT
DrdI 2637 GACTCCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2675 GACCTGCTGGTC
DsaI 453 CCATGG C^C[GA][CT]GG
5077 CCGTGG
EcoRV 4667 GATATC GAT^ATC
4879 GATATC
FspI 1674 TGCGCA TGC^GCA
2856 TGCGCA
SmaI 3540 CCCGGG CCC^GGG
3572 CCCGGG
XcmI 1545 CCACTGCTGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5123 CCAGAAGGAGATTGG
XmaCI 3540 CCCGGG C^CCGGG
3572 CCCGGG
XmaI 3540 CCCGGG C^CCGGG
3572 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApoI Asp718I AspHI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvCI BbvI BciVI BclI BcnI
BfaI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII