##################################
# NO CUT ENZYMES (   40 enzymes) #
##################################

AclI        AcsI        AflII       AhdI        ApoI        
AscI        AspEI       BciVI       BfrI        BsaBI       
BsiWI       BsmI        BspHI       BsrDI       BstBI       
Eam1105I    EcoRI       EcoRV       HindIII     HpaI        
KspI        MamI        MfeI        MluI        MunI        
NheI        NotI        NruI        NspV        PacI        
PmeI        Psp1406I    RcaI        SacII       SalI        
SfuI        SnaBI       SpeI        SspI        XbaI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AatII        2637  GACGTC               GACGT^C
AseI         7471  ATTAAT               AT^TAAT
AsnI         7471  ATTAAT               AT^TAAT
Asp700I      4895  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII         947  CCTAGG               C^CTAGG
BclI         2788  TGATCA               T^GATCA
BglII        2071  AGATCT               A^GATCT
BlnI          947  CCTAGG               C^CTAGG
BspLU11I      772  ACATGT               A^CATGT
Bst1107I     4514  GTATAC               GTA^TAC
BstZ17I      4514  GTATAC               GTA^TAC
DraI         6731  TTTAAA               TTT^AAA
EcoT22I      5676  ATGCAT               ATGCA^T
FbaI         2788  TGATCA               T^GATCA
NdeI         5652  CATATG               CA^TATG
NsiI         5676  ATGCAT               ATGCA^T
PshAI        2478  GACCTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        7471  ATTAAT               AT^TAAT
PvuI         4939  CGATCG               CGAT^CG
ScaI          253  AGTACT               AGT^ACT
SgrAI        4259  CACCGGCG             C[GA]^CCGG[CT]G
SphI         5674  GCATGC               GCATG^C
SwaI         6730  ATTTAAAT             ATTT^AAAT
XmnI         4895  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccI         3792  GTCTAC               GT^[AC][GT]AC
             4514  GTATAC              
AccIII       2122  TCCGGA               T^CCGGA
             4631  TCCGGA              
AgeI         4195  ACCGGT               A^CCGGT
             5617  ACCGGT              
Aor51HI      3325  AGCGCT               AGC^GCT
             3910  AGCGCT              
AviII         809  TGCGCA               TGC^GCA
             7263  TGCGCA              
BamHI        3807  GGATCC               G^GATCC
             5254  GGATCC              
BfaI          948  CTAG                 C^TAG
             4725  CTAG                
BseAI        2122  TCCGGA               T^CCGGA
             4631  TCCGGA              
Bsp1407I      208  TGTACA               T^GTACA
             5128  TGTACA              
BspDI         393  ATCGAT               AT^CGAT
             3504  ATCGAT              
BspEI        2122  TCCGGA               T^CCGGA
             4631  TCCGGA              
BsrGI         208  TGTACA               T^GTACA
             5128  TGTACA              
BssHII       1763  GCGCGC               G^CGCGC
             3567  GCGCGC              
ClaI          393  ATCGAT               AT^CGAT
             3504  ATCGAT              
CpoI          214  CGGACCG              CG^G[AT]CCG
             2901  CGGTCCG             
DraIII        318  CACCGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
              582  CACGAGGTG           
Eco47III     3325  AGCGCT               AGC^GCT
             3910  AGCGCT              
FseI         3756  GGCCGGCC             GGCCGG^CC
             4764  GGCCGGCC            
FspI          809  TGCGCA               TGC^GCA
             7263  TGCGCA              
HincII       4500  GTTGAC               GT[CT]^[GA]AC
             5472  GTTGAC              
HindII       4500  GTTGAC               GT[CT]^[GA]AC
             5472  GTTGAC              
MaeI          948  CTAG                 C^TAG
             4725  CTAG                
MroI         2122  TCCGGA               T^CCGGA
             4631  TCCGGA              
PaeR7I       4377  CTCGAG               C^TCGAG
             4431  CTCGAG              
PinAI        4195  ACCGGT               A^CCGGT
             5617  ACCGGT              
RsrII         214  CGGACCG              CG^G[AT]CCG
             2901  CGGTCCG             
SbfI         3294  CCTGCAGG             CCTGCA^GG
             4043  CCTGCAGG            
SexAI        4922  ACCAGGT              A^CC[AT]GGT
             5341  ACCTGGT             
SfiI          521  GGCCCGGGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3756  GGCCGGCCGGGCC       
Sse8387I     3294  CCTGCAGG             CCTGCA^GG
             4043  CCTGCAGG            
SspBI         208  TGTACA               T^GTACA
             5128  TGTACA              
XhoI         4377  CTCGAG               C^TCGAG
             4431  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  162 enzymes) #
#########################################

Acc65I      AccII       AciI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       Asp718I     AspHI       
AspI        AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BcnI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BssSI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DrdI        DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        KpnI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflFI       PflMI       PleI        
PmaCI       PmlI        PpuMI       PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmaI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI