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Gene/Protein Characteristic Table for FLJ00129

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Product ID	ORK05088
Accession No	AK074058
Clone name	sh01893
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (2432 bp) Predicted protein sequence (668 aa)
Source	Human spleen

Length: 2432 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 668 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR002048	40	92	PD000012	Calcium-binding EF-hand
HMMPfam	IPR002048	72	100	PF00036	Calcium-binding EF-hand
	IPR002916	166	667	PF01794	Ferric reductase-like transmembrane component
ProfileScan	IPR002048	40	97	PS50222	Calcium-binding EF-hand
ScanRegExp	IPR002048	81	93	PS00018	Calcium-binding EF-hand

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	142	LFCLATYAGLHVLLFGLAASAH	163	SECONDARY	22
	2	177	GQCLNFDCSFIAVLMLRRCLTWL	199	SECONDARY	23
	3	271	SPTGVALLLLLLFMFICSSSCI	292	PRIMARY	22
	4	309	LLVWLLLIFHGPNFWKWLLVPGI	331	PRIMARY	23
	5	337	KAIGLAVSRMAAVCIMEVNLLPS	359	SECONDARY	23

Experimental conditions

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