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Gene/Protein Characteristic Table for FLJ00414

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Product ID	ORK05197
Accession No	AK122589
Clone name	sg00016
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6453 bp) Predicted protein sequence (1129 aa)
HaloTag ORF Clone	FHC05197
Flexi ORF Clone	FXC05197
Source	Human spleen

Length: 6453 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 1129 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR001711	248	657	PD001202	Phosphatidylinositol-specific phospholipase C
FPrintScan	IPR001192	249	267	PR00390	Phosphoinositide-specific phospholipase C (PLC)
	IPR001192	275	295	PR00390	Phosphoinositide-specific phospholipase C (PLC)
	IPR001192	373	390	PR00390	Phosphoinositide-specific phospholipase C (PLC)
	IPR001192	596	617	PR00390	Phosphoinositide-specific phospholipase C (PLC)
	IPR001192	617	635	PR00390	Phosphoinositide-specific phospholipase C (PLC)
	IPR000008	699	711	PR00360	C2 domain
	IPR000008	729	742	PR00360	C2 domain
	IPR000008	751	759	PR00360	C2 domain
	IPR001192	771	781	PR00390	Phosphoinositide-specific phospholipase C (PLC)
HMMPfam	IPR002048	91	119	PF00036	Calcium-binding EF-hand
	IPR002048	127	156	PF00036	Calcium-binding EF-hand
	IPR000909	245	390	PF00388	Phosphatidylinositol-specific phospholipase C
	IPR001711	543	658	PF00387	Phosphatidylinositol-specific phospholipase C
	IPR000008	678	770	PF00168	C2 domain
HMMSmart	IPR002048	91	119	SM00054	Calcium-binding EF-hand
	IPR002048	127	156	SM00054	Calcium-binding EF-hand
	IPR000909	244	389	SM00148	Phosphatidylinositol-specific phospholipase C
	IPR001711	544	658	SM00149	Phosphatidylinositol-specific phospholipase C
	IPR000008	677	785	SM00239	C2 domain
ProfileScan	IPR001849	1	73	PS50003	Pleckstrin-like
	IPR002048	92	153	PS50222	Calcium-binding EF-hand
	IPR000909	244	389	PS50007	Phosphatidylinositol-specific phospholipase C
	IPR001472	488	505	PS50079	Bipartite nuclear localization signal
	IPR001711	544	657	PS50008	Phosphatidylinositol-specific phospholipase C
	IPR000008	663	770	PS50004	C2 domain
ScanRegExp	IPR002048	100	112	PS00018	Calcium-binding EF-hand

Experimental conditions

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