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Order Kazusa clone(s) from : |
| Product ID | ORK05067 |
|---|---|
| Accession No | AK024488 |
| Clone name | as00087 |
| Vector information | |
| cDNA sequence | DNA sequence (4287 bp) Predicted protein sequence (674 aa) |
| Source | Human spleen |
| Rouge ID |
mFLJ00087
by Kazusa Mouse cDNA Project
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Length: 4287 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | Warning |
Length: 674 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against other entries from Kazusa human cDNA project
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR000008 | 37 | 115 | PF00168 | C2 domain |
| IPR001936 | 210 | 382 | PF00616 | Ras GTPase-activating protein | |
| HMMSmart | IPR001936 | 138 | 460 | SM00323 | Ras GTPase-activating protein |
| ProfileScan | IPR001936 | 189 | 381 | PS50018 | Ras GTPase-activating protein |
| NULL | 520 | 560 | PS50323 | NULL |
RT-PCR-ELISA
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Experimental conditions| Primer_f | AGTACATGAAGCTCGTGGCAC |
|---|---|
| Primer_r | AGGGAACCAGTCGTAGGAATG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |