FLJ00324

Product ID	ORK05417
Accession No	AK131098
Clone name	sj01157
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4440 bp) Predicted protein sequence (376 aa)
Source	Human spleen
Rouge ID	mFLJ00324 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 376 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAC85148	1.1e-121	100.0	FLJ00324 protei...	Homo sapiens
AAH33838	8e-119	100.0	BRUNOL6 protein...	Homo sapiens
Q96J87	2e-112	100.0	CUG-BP- and ETR...	Homo sapiens
AAH30835	3.6e-112	99.7	Bruno-like 6, R...	Homo sapiens
XP_001092705	1.2e-111	99.4	similar to brun...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000504	31	102	PF00076	RNA-binding region RNP-1 (RNA recognition motif)
HMMPfam	IPR000504	293	364	PF00076	RNA-binding region RNP-1 (RNA recognition motif)
HMMSmart	IPR000504	30	105	SM00360	RNA-binding region RNP-1 (RNA recognition motif)
HMMSmart	IPR000504	292	365	SM00360	RNA-binding region RNP-1 (RNA recognition motif)
ProfileScan	IPR000504	29	109	PS50102	RNA-binding region RNP-1 (RNA recognition motif)
	NULL	137	190	PS50310	NULL
	IPR000504	291	369	PS50102	RNA-binding region RNP-1 (RNA recognition motif)

Primer_f
Primer_r
PCR conditions	test