FLJ00171

Product ID	ORK06874
Accession No	AK074100
Clone name	sj00104
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4653 bp) Predicted protein sequence (396 aa)
Source	Human spleen
Rouge ID	mFLJ00171 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Length: 396 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84926	2.7e-172	100.0	FLJ00171 protei...	Homo sapiens
EAW67620	8e-168	100.0	solute carrier ...	Homo sapiens
Q8TED4	4.2e-146	100.0	Sugar phosphate...	Homo sapiens
AAH51314	4.2e-146	100.0	Solute carrier ...	Homo sapiens
XP_508843	1e-145	99.1	hypothetical pr...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
ProfileScan	IPR007114	36	396	PS50850	Major facilitator superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	35	LILLLTFLIYACYHMSRKPISIV	57	SECONDARY	23
	2	99	GGVDNAFLIAYAIGMFISGVFGE	121	SECONDARY	23
	3	126	RYYLSAGMLLSGLFTSLFGLGYF	148	PRIMARY	23
	4	157	FVVIQVCNGLVQTTGWPSVVTCV	179	SECONDARY	23
	5	190	FIMGIWNSHTSVGNILGSLIAGI	212	SECONDARY	23
	6	220	LSFIVPGIITAVMGVITFLFLIE	242	PRIMARY	23
	7	292	EEPAAISFFGALRIPGVVEFSLC	314	SECONDARY	23
	8	318	AKLVSYTFLYWLPLYIANVAHFS	340	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test