Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC006439A_C01 KMC006439A_c01
(552 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9XHM1|IF38_MEDTR Eukaryotic translation initiation factor 3 ... 50 2e-05
pir||T50775 probable translation initiation factor [imported] - ... 50 2e-05
sp|P29834|GRP2_ORYSA Glycine-rich cell wall structural protein 2... 31 0.005
emb|CAA52788.1| keratin 2 epidermis [Mus musculus] 31 0.013
pir||A60830 keratin, 70k type II, epidermal - mouse (fragment) g... 31 0.014
>sp|Q9XHM1|IF38_MEDTR Eukaryotic translation initiation factor 3 subunit 8 (eIF3 p110)
(eIF3c) gi|11282366|pir||T50774 probable translation
initiation factor [imported] - barrel medic
gi|5106926|gb|AAD39891.1|AF106930_1 putative translation
initiation protein [Medicago truncatula]
Length = 935
Score = 49.7 bits (117), Expect = 2e-05
Identities = 31/50 (62%), Positives = 33/50 (66%), Gaps = 1/50 (2%)
Frame = -1
Query: 444 GIERDGGTGGG-YQSAYSGGSGRAHHGGSTLRGHQGDVSTRMVSLKGVRA 298
G R GTGGG YQ+ SGR GGS LRG GD STRMVSL+GVRA
Sbjct: 892 GYSRGRGTGGGGYQN-----SGRTQ-GGSALRGPHGDTSTRMVSLRGVRA 935
Score = 48.9 bits (115), Expect = 4e-05
Identities = 29/47 (61%), Positives = 33/47 (69%), Gaps = 2/47 (4%)
Frame = -3
Query: 550 DLSLSQPXQGGGRTGYGSGGGXPMAFSQAAGS-GYSRDR-TGRGNWR 416
DLS SQ QG GRTGY GGG ++FSQA GS GYSR R TG G ++
Sbjct: 861 DLSYSQTRQGSGRTGY--GGGRALSFSQAGGSGGYSRGRGTGGGGYQ 905
>pir||T50775 probable translation initiation factor [imported] - barrel medic
gi|5106928|gb|AAD39892.1|AF106931_1 putative translation
initiation protein [Medicago truncatula]
Length = 383
Score = 49.7 bits (117), Expect = 2e-05
Identities = 28/54 (51%), Positives = 33/54 (60%), Gaps = 4/54 (7%)
Frame = -1
Query: 447 LGIERDGGTGGGYQSAYSGGSGRAH----HGGSTLRGHQGDVSTRMVSLKGVRA 298
L + GG+GG + GG G + GGS LRG GDVSTRMVSL+GVRA
Sbjct: 330 LSFNQAGGSGGYSRGRGMGGGGYQNSSRTQGGSALRGPHGDVSTRMVSLRGVRA 383
Score = 40.8 bits (94), Expect = 0.011
Identities = 23/39 (58%), Positives = 26/39 (65%), Gaps = 1/39 (2%)
Frame = -3
Query: 550 DLSLSQPXQGGGRTGYGSGGGXPMAFSQAAGS-GYSRDR 437
DLS SQ QG GR GY GG ++F+QA GS GYSR R
Sbjct: 310 DLSYSQTRQGSGRAGY---GGRALSFNQAGGSGGYSRGR 345
>sp|P29834|GRP2_ORYSA Glycine-rich cell wall structural protein 2 precursor
gi|72322|pir||KNRZG2 glycine-rich cell wall structural
protein 2 precursor - rice gi|20245|emb|CAA38315.1|
Glycine-rich protein [Oryza sativa]
gi|1167557|gb|AAA85863.1| glycine-rich protein
Length = 183
Score = 31.2 bits (69), Expect(2) = 0.005
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = -3
Query: 523 GGGRTGYGSGGGXPMAFSQAAGSGYSRDRTGRGNW 419
GGG G G GGG + GSGY G G +
Sbjct: 75 GGGGGGGGEGGGSGSGYGSGQGSGYGAGVGGAGGY 109
Score = 30.0 bits (66), Expect(2) = 0.005
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = -1
Query: 426 GTGGGYQSAYSGGSGRAHHGG 364
G G GY S Y G+G AH GG
Sbjct: 127 GQGSGYGSGYGSGAGGAHGGG 147
Score = 28.5 bits (62), Expect(2) = 0.23
Identities = 11/26 (42%), Positives = 13/26 (49%)
Frame = -3
Query: 526 QGGGRTGYGSGGGXPMAFSQAAGSGY 449
QGGG GYG G G + AG +
Sbjct: 119 QGGGAGGYGQGSGYGSGYGSGAGGAH 144
Score = 28.5 bits (62), Expect(2) = 0.84
Identities = 15/32 (46%), Positives = 16/32 (49%)
Frame = -3
Query: 520 GGRTGYGSGGGXPMAFSQAAGSGYSRDRTGRG 425
G +GYG GGG AAG GY R G G
Sbjct: 52 GSGSGYGEGGGS----GGAAGGGYGRGGGGGG 79
Score = 27.7 bits (60), Expect(2) = 0.50
Identities = 12/20 (60%), Positives = 12/20 (60%)
Frame = -3
Query: 508 GYGSGGGXPMAFSQAAGSGY 449
GYGSGGG Q GSGY
Sbjct: 147 GYGSGGGGGGGGGQGGGSGY 166
Score = 26.9 bits (58), Expect(2) = 0.23
Identities = 12/23 (52%), Positives = 12/23 (52%)
Frame = -1
Query: 429 GGTGGGYQSAYSGGSGRAHHGGS 361
G GGGY S GG G GGS
Sbjct: 142 GAHGGGYGSGGGGGGGGGQGGGS 164
Score = 26.6 bits (57), Expect(2) = 0.50
Identities = 11/19 (57%), Positives = 13/19 (67%)
Frame = -1
Query: 426 GTGGGYQSAYSGGSGRAHH 370
G+G GY S Y GG+G HH
Sbjct: 167 GSGSGYGSGYGGGNG--HH 183
Score = 25.8 bits (55), Expect(2) = 6.3
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = -1
Query: 429 GGTGGGYQSAYSGGSGRAHHGGS 361
G GGGY GG G GGS
Sbjct: 65 GAAGGGYGRGGGGGGGGGEGGGS 87
Score = 25.0 bits (53), Expect(2) = 0.84
Identities = 10/22 (45%), Positives = 11/22 (49%)
Frame = -1
Query: 429 GGTGGGYQSAYSGGSGRAHHGG 364
GG GGG S Y G G + G
Sbjct: 81 GGEGGGSGSGYGSGQGSGYGAG 102
Score = 24.6 bits (52), Expect(2) = 6.3
Identities = 13/28 (46%), Positives = 14/28 (49%)
Frame = -3
Query: 532 PXQGGGRTGYGSGGGXPMAFSQAAGSGY 449
P GGG G G GGG +GSGY
Sbjct: 31 PGGGGGGGGEGGGGGYG-GSGYGSGSGY 57
>emb|CAA52788.1| keratin 2 epidermis [Mus musculus]
Length = 707
Score = 31.2 bits (69), Expect(2) = 0.013
Identities = 14/30 (46%), Positives = 16/30 (52%)
Frame = -1
Query: 429 GGTGGGYQSAYSGGSGRAHHGGSTLRGHQG 340
GG+GGGY S GSG + G RG G
Sbjct: 608 GGSGGGYGSGGGSGSGGGYSSGGGSRGGSG 637
Score = 28.5 bits (62), Expect(2) = 0.96
Identities = 15/34 (44%), Positives = 17/34 (49%)
Frame = -3
Query: 526 QGGGRTGYGSGGGXPMAFSQAAGSGYSRDRTGRG 425
+GG GYGSGGG +G GYS RG
Sbjct: 607 RGGSGGGYGSGGG------SGSGGGYSSGGGSRG 634
Score = 28.5 bits (62), Expect(2) = 0.013
Identities = 16/36 (44%), Positives = 19/36 (52%), Gaps = 3/36 (8%)
Frame = -3
Query: 523 GGGRTGYGSGG---GXPMAFSQAAGSGYSRDRTGRG 425
GGG + YGSGG G + S + G GYS RG
Sbjct: 559 GGGGSSYGSGGRSSGSRGSGSGSGGGGYSSGGGSRG 594
Score = 25.8 bits (55), Expect(2) = 5.6
Identities = 12/25 (48%), Positives = 13/25 (52%)
Frame = -3
Query: 526 QGGGRTGYGSGGGXPMAFSQAAGSG 452
+GG GYGSGGG GSG
Sbjct: 593 RGGSGGGYGSGGGSRGGSGGGYGSG 617
Score = 24.6 bits (52), Expect(2) = 0.96
Identities = 11/27 (40%), Positives = 15/27 (54%)
Frame = -1
Query: 429 GGTGGGYQSAYSGGSGRAHHGGSTLRG 349
GG+GGG S+ G G + GG + G
Sbjct: 634 GGSGGGGASSGGGSRGGSSSGGGSRGG 660
Score = 24.6 bits (52), Expect(2) = 5.6
Identities = 13/27 (48%), Positives = 15/27 (55%), Gaps = 5/27 (18%)
Frame = -1
Query: 426 GTGGGYQS-----AYSGGSGRAHHGGS 361
G+GGGY S SGG G + GGS
Sbjct: 621 GSGGGYSSGGGSRGGSGGGGASSGGGS 647
>pir||A60830 keratin, 70k type II, epidermal - mouse (fragment)
gi|387392|gb|AAA39370.1| keratin [Mus musculus]
Length = 270
Score = 31.2 bits (69), Expect(2) = 0.014
Identities = 14/30 (46%), Positives = 16/30 (52%)
Frame = -1
Query: 429 GGTGGGYQSAYSGGSGRAHHGGSTLRGHQG 340
GG+GGGY S GSG + G RG G
Sbjct: 177 GGSGGGYGSGGGSGSGGGYSSGGGSRGGSG 206
Score = 28.5 bits (62), Expect(2) = 1.0
Identities = 15/34 (44%), Positives = 17/34 (49%)
Frame = -3
Query: 526 QGGGRTGYGSGGGXPMAFSQAAGSGYSRDRTGRG 425
+GG GYGSGGG +G GYS RG
Sbjct: 176 RGGSGGGYGSGGG------SGSGGGYSSGGGSRG 203
Score = 28.5 bits (62), Expect(2) = 0.014
Identities = 16/36 (44%), Positives = 19/36 (52%), Gaps = 3/36 (8%)
Frame = -3
Query: 523 GGGRTGYGSGG---GXPMAFSQAAGSGYSRDRTGRG 425
GGG + YGSGG G + S + G GYS RG
Sbjct: 128 GGGGSSYGSGGRSSGSRGSGSGSGGGGYSSGGGSRG 163
Score = 25.8 bits (55), Expect(2) = 6.0
Identities = 12/25 (48%), Positives = 13/25 (52%)
Frame = -3
Query: 526 QGGGRTGYGSGGGXPMAFSQAAGSG 452
+GG GYGSGGG GSG
Sbjct: 162 RGGSGGGYGSGGGSRGGSGGGYGSG 186
Score = 24.6 bits (52), Expect(2) = 1.0
Identities = 11/27 (40%), Positives = 15/27 (54%)
Frame = -1
Query: 429 GGTGGGYQSAYSGGSGRAHHGGSTLRG 349
GG+GGG S+ G G + GG + G
Sbjct: 203 GGSGGGGASSGGGSRGGSSSGGGSRGG 229
Score = 24.6 bits (52), Expect(2) = 6.0
Identities = 13/27 (48%), Positives = 15/27 (55%), Gaps = 5/27 (18%)
Frame = -1
Query: 426 GTGGGYQS-----AYSGGSGRAHHGGS 361
G+GGGY S SGG G + GGS
Sbjct: 190 GSGGGYSSGGGSRGGSGGGGASSGGGS 216
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 491,708,965
Number of Sequences: 1393205
Number of extensions: 11929766
Number of successful extensions: 81503
Number of sequences better than 10.0: 466
Number of HSP's better than 10.0 without gapping: 44761
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 75080
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19234190289
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)