Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC003127A_C01 KMC003127A_c01
(642 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q41009|TO34_PEA Translocase of chloroplast 34 (34 kDa chlorop... 109 1e-25
pir||B55171 chloroplast import-associated protein IAP36, GTP-bin... 107 5e-25
ref|NP_196119.1| GTP-binding protein (gb|AAD09203.1); protein id... 79 5e-17
gb|AAM65983.1| GTP-binding protein [Arabidopsis thaliana] 79 5e-17
sp|Q38906|TO34_ARATH Translocase of chloroplast 34 (34 kDa chlor... 79 7e-16
>sp|Q41009|TO34_PEA Translocase of chloroplast 34 (34 kDa chloroplast outer envelope
protein) (GTP-binding protein OEP34) (GTP-binding
protein IAP34) gi|510190|emb|CAA82196.1| chloroplast
outer envelope protein 34 [Pisum sativum]
gi|3293201|gb|AAC25785.1| GTP-binding protein [Pisum
sativum]
Length = 310
Score = 109 bits (273), Expect(2) = 1e-25
Identities = 51/67 (76%), Positives = 59/67 (87%)
Frame = -2
Query: 641 PNGIAWISNLVQKITKVALNKSESIHVDKNLIEGPNPNQTGKLWIPLLYALPYFFVIKPI 462
PNGIAWI +LVQ IT+VALNKSESI VDKNLI+GPNPNQ GKLWIPL++AL Y F+ KPI
Sbjct: 226 PNGIAWIPHLVQTITEVALNKSESIFVDKNLIDGPNPNQRGKLWIPLIFALQYLFLAKPI 285
Query: 461 EGLIQQD 441
E LI++D
Sbjct: 286 EALIRRD 292
Score = 28.5 bits (62), Expect(2) = 1e-25
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = -3
Query: 445 RTLSSESRPSWEMRDAGLRK 386
R +++E++P+WE RD G RK
Sbjct: 291 RDIATETKPAWETRDVGDRK 310
>pir||B55171 chloroplast import-associated protein IAP36, GTP-binding - garden
pea
Length = 310
Score = 107 bits (268), Expect(2) = 5e-25
Identities = 50/67 (74%), Positives = 58/67 (85%)
Frame = -2
Query: 641 PNGIAWISNLVQKITKVALNKSESIHVDKNLIEGPNPNQTGKLWIPLLYALPYFFVIKPI 462
PNGI WI +LVQ IT+VALNKSESI VDKNLI+GPNPNQ GKLWIPL++AL Y F+ KPI
Sbjct: 226 PNGIRWIPHLVQTITEVALNKSESIFVDKNLIDGPNPNQRGKLWIPLIFALQYLFLAKPI 285
Query: 461 EGLIQQD 441
E LI++D
Sbjct: 286 EALIRRD 292
Score = 28.5 bits (62), Expect(2) = 5e-25
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = -3
Query: 445 RTLSSESRPSWEMRDAGLRK 386
R +++E++P+WE RD G RK
Sbjct: 291 RDIATETKPAWETRDVGDRK 310
>ref|NP_196119.1| GTP-binding protein (gb|AAD09203.1); protein id: At5g05000.2,
supported by cDNA: 6734. [Arabidopsis thaliana]
gi|10178039|dbj|BAB11522.1| GTP-binding protein
[Arabidopsis thaliana]
Length = 313
Score = 79.0 bits (193), Expect(2) = 5e-17
Identities = 34/67 (50%), Positives = 48/67 (70%)
Frame = -2
Query: 641 PNGIAWISNLVQKITKVALNKSESIHVDKNLIEGPNPNQTGKLWIPLLYALPYFFVIKPI 462
P G +WI NL KIT+++ N +++IHVDK L+EGPNPN+ GK IPL++A Y V+KP+
Sbjct: 226 PCGTSWIPNLFNKITEISFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPL 285
Query: 461 EGLIQQD 441
I+ D
Sbjct: 286 VRAIKSD 292
Score = 30.4 bits (67), Expect(2) = 5e-17
Identities = 11/19 (57%), Positives = 16/19 (83%)
Frame = -3
Query: 439 LSSESRPSWEMRDAGLRKR 383
+S ES+P+WE+RD+GL R
Sbjct: 293 VSRESKPAWELRDSGLASR 311
>gb|AAM65983.1| GTP-binding protein [Arabidopsis thaliana]
Length = 313
Score = 79.0 bits (193), Expect(2) = 5e-17
Identities = 34/67 (50%), Positives = 48/67 (70%)
Frame = -2
Query: 641 PNGIAWISNLVQKITKVALNKSESIHVDKNLIEGPNPNQTGKLWIPLLYALPYFFVIKPI 462
P G +WI NL KIT+++ N +++IHVDK L+EGPNPN+ GK IPL++A Y V+KP+
Sbjct: 226 PCGTSWIPNLFNKITEISFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPL 285
Query: 461 EGLIQQD 441
I+ D
Sbjct: 286 VRAIKSD 292
Score = 30.4 bits (67), Expect(2) = 5e-17
Identities = 11/19 (57%), Positives = 16/19 (83%)
Frame = -3
Query: 439 LSSESRPSWEMRDAGLRKR 383
+S ES+P+WE+RD+GL R
Sbjct: 293 VSRESKPAWELRDSGLASR 311
>sp|Q38906|TO34_ARATH Translocase of chloroplast 34 (34 kDa chloroplast outer envelope
protein) (GTP-binding protein OEP34) (AtToc34)
gi|1151244|gb|AAD09203.1| GTP-binding protein
[Arabidopsis thaliana] gi|11557975|emb|CAC17699.1|
atToc34 protein [Arabidopsis thaliana]
Length = 313
Score = 79.0 bits (193), Expect(2) = 7e-16
Identities = 34/67 (50%), Positives = 48/67 (70%)
Frame = -2
Query: 641 PNGIAWISNLVQKITKVALNKSESIHVDKNLIEGPNPNQTGKLWIPLLYALPYFFVIKPI 462
P G +WI NL KIT+++ N +++IHVDK L+EGPNPN+ GK IPL++A Y V+KP+
Sbjct: 226 PCGTSWIPNLFNKITEISFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPL 285
Query: 461 EGLIQQD 441
I+ D
Sbjct: 286 VRAIKSD 292
Score = 26.6 bits (57), Expect(2) = 7e-16
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = -3
Query: 439 LSSESRPSWEMRDAGLRKR 383
+S ES+ +WE+RD+GL R
Sbjct: 293 VSRESKLAWELRDSGLASR 311
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 568,168,705
Number of Sequences: 1393205
Number of extensions: 12134404
Number of successful extensions: 28962
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 28284
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28956
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 27007650415
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
