KMC003127A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC003127A_C01 KMC003127A_c01
gtgctagcccaaaccatatcaactcttacttaagaagaagctaccatatcccccctagta
ccagtagtccctgggggGCTGTGCAAGAGTTAGGAATATTTCATGATAACAATGAATCAA
AAACTTCAGACCAGTGTTATAGAATATGATAGCAGCCACATATAGTGCTATGTCTGAGAA
ATGGAATACTACCAAACTGGGTTATGCAAAAGCAAACTACAACACAATCCTCTTAAAAGC
ACAAATAAATTCCTACGCCGCAGACTACTAGGAATTGCAGTACATGGAAACACAATCTAA
ATTCACCCGGAACCCAGATGACAATGGACAGGATGGAATTGCATGAGGACAAAGATGCCA
GCTAATAGAGATCCCTTTGATCTCTTTTGCGTAAACCTGCATCTCGCATCTCCCATGAAG
GCCTACTCTCACTTGAAAGTGTCCTGCTGGATCAGTCCTTCTATCGGCTTTATCACAAAG
AAATATGGTAATGCATACAGGAGAGGAATCCATAATTTTCCAGTCTGATTTGGATTTGGC
CCTTCAATCAAATTCTTATCAACATGAATAGACTCACTTTTGTTCAATGCAACTTTTGTG
ATTTTTTGGACTAGATTAGAAATCCATGCAATCCCATTTGGA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC003127A_C01 KMC003127A_c01
         (642 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

sp|Q41009|TO34_PEA Translocase of chloroplast 34 (34 kDa chlorop...   109  1e-25
pir||B55171 chloroplast import-associated protein IAP36, GTP-bin...   107  5e-25
ref|NP_196119.1| GTP-binding protein (gb|AAD09203.1); protein id...    79  5e-17
gb|AAM65983.1| GTP-binding protein [Arabidopsis thaliana]              79  5e-17
sp|Q38906|TO34_ARATH Translocase of chloroplast 34 (34 kDa chlor...    79  7e-16

>sp|Q41009|TO34_PEA Translocase of chloroplast 34 (34 kDa chloroplast outer envelope
           protein) (GTP-binding protein OEP34) (GTP-binding
           protein IAP34) gi|510190|emb|CAA82196.1| chloroplast
           outer envelope protein 34 [Pisum sativum]
           gi|3293201|gb|AAC25785.1| GTP-binding protein [Pisum
           sativum]
          Length = 310

 Score =  109 bits (273), Expect(2) = 1e-25
 Identities = 51/67 (76%), Positives = 59/67 (87%)
 Frame = -2

Query: 641 PNGIAWISNLVQKITKVALNKSESIHVDKNLIEGPNPNQTGKLWIPLLYALPYFFVIKPI 462
           PNGIAWI +LVQ IT+VALNKSESI VDKNLI+GPNPNQ GKLWIPL++AL Y F+ KPI
Sbjct: 226 PNGIAWIPHLVQTITEVALNKSESIFVDKNLIDGPNPNQRGKLWIPLIFALQYLFLAKPI 285

Query: 461 EGLIQQD 441
           E LI++D
Sbjct: 286 EALIRRD 292

 Score = 28.5 bits (62), Expect(2) = 1e-25
 Identities = 10/20 (50%), Positives = 16/20 (80%)
 Frame = -3

Query: 445 RTLSSESRPSWEMRDAGLRK 386
           R +++E++P+WE RD G RK
Sbjct: 291 RDIATETKPAWETRDVGDRK 310

>pir||B55171 chloroplast import-associated protein IAP36, GTP-binding - garden
           pea
          Length = 310

 Score =  107 bits (268), Expect(2) = 5e-25
 Identities = 50/67 (74%), Positives = 58/67 (85%)
 Frame = -2

Query: 641 PNGIAWISNLVQKITKVALNKSESIHVDKNLIEGPNPNQTGKLWIPLLYALPYFFVIKPI 462
           PNGI WI +LVQ IT+VALNKSESI VDKNLI+GPNPNQ GKLWIPL++AL Y F+ KPI
Sbjct: 226 PNGIRWIPHLVQTITEVALNKSESIFVDKNLIDGPNPNQRGKLWIPLIFALQYLFLAKPI 285

Query: 461 EGLIQQD 441
           E LI++D
Sbjct: 286 EALIRRD 292

 Score = 28.5 bits (62), Expect(2) = 5e-25
 Identities = 10/20 (50%), Positives = 16/20 (80%)
 Frame = -3

Query: 445 RTLSSESRPSWEMRDAGLRK 386
           R +++E++P+WE RD G RK
Sbjct: 291 RDIATETKPAWETRDVGDRK 310

>ref|NP_196119.1| GTP-binding protein (gb|AAD09203.1); protein id: At5g05000.2,
           supported by cDNA: 6734. [Arabidopsis thaliana]
           gi|10178039|dbj|BAB11522.1| GTP-binding protein
           [Arabidopsis thaliana]
          Length = 313

 Score = 79.0 bits (193), Expect(2) = 5e-17
 Identities = 34/67 (50%), Positives = 48/67 (70%)
 Frame = -2

Query: 641 PNGIAWISNLVQKITKVALNKSESIHVDKNLIEGPNPNQTGKLWIPLLYALPYFFVIKPI 462
           P G +WI NL  KIT+++ N +++IHVDK L+EGPNPN+ GK  IPL++A  Y  V+KP+
Sbjct: 226 PCGTSWIPNLFNKITEISFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPL 285

Query: 461 EGLIQQD 441
              I+ D
Sbjct: 286 VRAIKSD 292

 Score = 30.4 bits (67), Expect(2) = 5e-17
 Identities = 11/19 (57%), Positives = 16/19 (83%)
 Frame = -3

Query: 439 LSSESRPSWEMRDAGLRKR 383
           +S ES+P+WE+RD+GL  R
Sbjct: 293 VSRESKPAWELRDSGLASR 311

>gb|AAM65983.1| GTP-binding protein [Arabidopsis thaliana]
          Length = 313

 Score = 79.0 bits (193), Expect(2) = 5e-17
 Identities = 34/67 (50%), Positives = 48/67 (70%)
 Frame = -2

Query: 641 PNGIAWISNLVQKITKVALNKSESIHVDKNLIEGPNPNQTGKLWIPLLYALPYFFVIKPI 462
           P G +WI NL  KIT+++ N +++IHVDK L+EGPNPN+ GK  IPL++A  Y  V+KP+
Sbjct: 226 PCGTSWIPNLFNKITEISFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPL 285

Query: 461 EGLIQQD 441
              I+ D
Sbjct: 286 VRAIKSD 292

 Score = 30.4 bits (67), Expect(2) = 5e-17
 Identities = 11/19 (57%), Positives = 16/19 (83%)
 Frame = -3

Query: 439 LSSESRPSWEMRDAGLRKR 383
           +S ES+P+WE+RD+GL  R
Sbjct: 293 VSRESKPAWELRDSGLASR 311

>sp|Q38906|TO34_ARATH Translocase of chloroplast 34 (34 kDa chloroplast outer envelope
           protein) (GTP-binding protein OEP34) (AtToc34)
           gi|1151244|gb|AAD09203.1| GTP-binding protein
           [Arabidopsis thaliana] gi|11557975|emb|CAC17699.1|
           atToc34 protein [Arabidopsis thaliana]
          Length = 313

 Score = 79.0 bits (193), Expect(2) = 7e-16
 Identities = 34/67 (50%), Positives = 48/67 (70%)
 Frame = -2

Query: 641 PNGIAWISNLVQKITKVALNKSESIHVDKNLIEGPNPNQTGKLWIPLLYALPYFFVIKPI 462
           P G +WI NL  KIT+++ N +++IHVDK L+EGPNPN+ GK  IPL++A  Y  V+KP+
Sbjct: 226 PCGTSWIPNLFNKITEISFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPL 285

Query: 461 EGLIQQD 441
              I+ D
Sbjct: 286 VRAIKSD 292

 Score = 26.6 bits (57), Expect(2) = 7e-16
 Identities = 10/19 (52%), Positives = 15/19 (78%)
 Frame = -3

Query: 439 LSSESRPSWEMRDAGLRKR 383
           +S ES+ +WE+RD+GL  R
Sbjct: 293 VSRESKLAWELRDSGLASR 311

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 568,168,705
Number of Sequences: 1393205
Number of extensions: 12134404
Number of successful extensions: 28962
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 28284
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28956
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 27007650415
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 GNf022b01 BP068944 1 503
2 MF011h02_f BP028830 78 432
3 MR100c12_f BP083644 95 580
4 MPD040e08_f AV772743 99 565
5 MPDL018b05_f AV777387 105 643
6 MR076h09_f BP081889 108 468
7 MWM015b05_f AV764824 129 638
8 GNf021g05 BP068917 142 560




Lotus japonicus
Kazusa DNA Research Institute