Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC002986A_C01 KMC002986A_c01
(989 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_195236.1| plasma membrane intrinsic protein (SIMIP); prot... 362 e-122
gb|AAF71820.1|AF141900_1 putative aquaporin PIP2-2 [Vitis berlan... 355 e-121
gb|AAG30607.1|AF314656_1 aquaporin [Brassica oleracea] 362 e-121
gb|AAB36949.1| plasma membrane intrinsic protein PIP3 [Arabidops... 356 e-120
gb|AAB65787.1| plasma membrane intrinsic protein [Arabidopsis th... 356 e-120
>ref|NP_195236.1| plasma membrane intrinsic protein (SIMIP); protein id: At4g35100.1,
supported by cDNA: 7108., supported by cDNA:
gi_15724339, supported by cDNA: gi_1688295, supported by
cDNA: gi_17065453, supported by cDNA: gi_20148502,
supported by cDNA: gi_2306916 [Arabidopsis thaliana]
gi|7441242|pir||T05780 plasma membrane intrinsic protein
M4E13.150 - Arabidopsis thaliana
gi|2924520|emb|CAA17774.1| plasma membrane intrinsic
protein (SIMIP) [Arabidopsis thaliana]
gi|7270461|emb|CAB80227.1| plasma membrane intrinsic
protein (SIMIP) [Arabidopsis thaliana]
gi|15724340|gb|AAL06563.1|AF412110_1 AT4g35100/M4E13_150
[Arabidopsis thaliana] gi|17065454|gb|AAL32881.1| plasma
membrane intrinsic protein (SIMIP) [Arabidopsis
thaliana] gi|20148503|gb|AAM10142.1| plasma membrane
intrinsic protein (SIMIP) [Arabidopsis thaliana]
gi|21594556|gb|AAM66021.1| plasma membrane intrinsic
protein SIMIP [Arabidopsis thaliana]
Length = 280
Score = 362 bits (928), Expect(4) = e-122
Identities = 175/197 (88%), Positives = 188/197 (94%)
Frame = -1
Query: 917 LAEIKLWSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAWSFGGMIFVL 738
+ E+K WSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAW+FGGMIFVL
Sbjct: 28 MGELKSWSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAWAFGGMIFVL 87
Query: 737 VYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGLVKAFMKHPYNSL 558
VYCTAGISGGHINPAVTFGLFLARKVSL+RA+ YM+AQCLGAICGVG VKAFMK PYN+L
Sbjct: 88 VYCTAGISGGHINPAVTFGLFLARKVSLVRALGYMIAQCLGAICGVGFVKAFMKTPYNTL 147
Query: 557 GGGANSVAHGYSKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFM 378
GGGAN+VA GYSKG+ALGAEIIGTFVLVYTVFSATDPKRSARDSH+PVLAPLPIGFAVFM
Sbjct: 148 GGGANTVADGYSKGTALGAEIIGTFVLVYTVFSATDPKRSARDSHIPVLAPLPIGFAVFM 207
Query: 377 VHLAPIPLTGTGLKPCQ 327
VHLA IP+TGTG+ P +
Sbjct: 208 VHLATIPITGTGINPAR 224
Score = 85.5 bits (210), Expect(4) = e-122
Identities = 37/53 (69%), Positives = 44/53 (82%)
Frame = -2
Query: 340 LSPARSFGAAVIFNHGQVWDDPWIFWVGPFVGALAAAAYHPYILRARGYQGTG 182
++PARSFGAAVI+N+ + WDD WIFWVGPF+GALAAAAYH YILRA + G
Sbjct: 220 INPARSFGAAVIYNNEKAWDDQWIFWVGPFLGALAAAAYHQYILRASAIKALG 272
Score = 33.1 bits (74), Expect(4) = e-122
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = -3
Query: 987 LKERRKREMSKDYVDPPPAPLIE 919
+ E K KDYVDPPPAPL++
Sbjct: 5 VSEEGKTHHGKDYVDPPPAPLLD 27
Score = 25.0 bits (53), Expect(4) = e-122
Identities = 11/12 (91%), Positives = 12/12 (99%)
Frame = -3
Query: 201 AAIKALGSFRSN 166
+AIKALGSFRSN
Sbjct: 266 SAIKALGSFRSN 277
>gb|AAF71820.1|AF141900_1 putative aquaporin PIP2-2 [Vitis berlandieri x Vitis rupestris]
Length = 279
Score = 355 bits (910), Expect(4) = e-121
Identities = 174/197 (88%), Positives = 188/197 (95%)
Frame = -1
Query: 917 LAEIKLWSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAWSFGGMIFVL 738
+AEIKLWSFYRA+IAEFIATLLFLY+TVATVIG+KKQ+ PC GVGLLGIAW+FGGMIF+L
Sbjct: 27 IAEIKLWSFYRAVIAEFIATLLFLYITVATVIGYKKQSDPCGGVGLLGIAWAFGGMIFIL 86
Query: 737 VYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGLVKAFMKHPYNSL 558
VYCTAGI+GGHINPAVTFGLFLARKVSLIRA+ YMVAQCLGAICGVGLVKAFMK YNSL
Sbjct: 87 VYCTAGIAGGHINPAVTFGLFLARKVSLIRALAYMVAQCLGAICGVGLVKAFMKSFYNSL 146
Query: 557 GGGANSVAHGYSKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFM 378
GGGANSVA GY+KG+ALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFM
Sbjct: 147 GGGANSVAAGYNKGTALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFM 206
Query: 377 VHLAPIPLTGTGLKPCQ 327
VHLA IP+TGTG+ P +
Sbjct: 207 VHLATIPITGTGINPAR 223
Score = 88.2 bits (217), Expect(4) = e-121
Identities = 39/53 (73%), Positives = 45/53 (84%)
Frame = -2
Query: 340 LSPARSFGAAVIFNHGQVWDDPWIFWVGPFVGALAAAAYHPYILRARGYQGTG 182
++PARSFGAAVI+N+ +VWDD WIFWVGPFVGALAAAAYH YILRA + G
Sbjct: 219 INPARSFGAAVIYNNEKVWDDQWIFWVGPFVGALAAAAYHQYILRAAAIKALG 271
Score = 31.6 bits (70), Expect(4) = e-121
Identities = 12/13 (92%), Positives = 13/13 (99%)
Frame = -3
Query: 957 KDYVDPPPAPLIE 919
KDYVDPPPAPLI+
Sbjct: 14 KDYVDPPPAPLID 26
Score = 28.9 bits (63), Expect(4) = e-121
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = -3
Query: 201 AAIKALGSFRSNP 163
AAIKALGSFRSNP
Sbjct: 265 AAIKALGSFRSNP 277
>gb|AAG30607.1|AF314656_1 aquaporin [Brassica oleracea]
Length = 281
Score = 362 bits (929), Expect(4) = e-121
Identities = 175/197 (88%), Positives = 188/197 (94%)
Frame = -1
Query: 917 LAEIKLWSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAWSFGGMIFVL 738
+ E+K WSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAW+FGGMIFVL
Sbjct: 29 MGELKSWSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAWAFGGMIFVL 88
Query: 737 VYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGLVKAFMKHPYNSL 558
VYCTAG+SGGHINPAVTFGLFLARKVSL+RAV YM+AQCLGAICGVG VKAFMK PYN+L
Sbjct: 89 VYCTAGVSGGHINPAVTFGLFLARKVSLVRAVGYMIAQCLGAICGVGFVKAFMKTPYNTL 148
Query: 557 GGGANSVAHGYSKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFM 378
GGGAN+VA GYSKG+ALGAEIIGTFVLVYTVFSATDPKRSARDSH+PVLAPLPIGFAVFM
Sbjct: 149 GGGANTVAPGYSKGTALGAEIIGTFVLVYTVFSATDPKRSARDSHIPVLAPLPIGFAVFM 208
Query: 377 VHLAPIPLTGTGLKPCQ 327
VHLA IP+TGTG+ P +
Sbjct: 209 VHLATIPITGTGINPAR 225
Score = 84.7 bits (208), Expect(4) = e-121
Identities = 37/46 (80%), Positives = 42/46 (90%)
Frame = -2
Query: 340 LSPARSFGAAVIFNHGQVWDDPWIFWVGPFVGALAAAAYHPYILRA 203
++PARSFGAAVI+N+ + WDD WIFWVGPFVGALAAAAYH YILRA
Sbjct: 221 INPARSFGAAVIYNNEKAWDDHWIFWVGPFVGALAAAAYHQYILRA 266
Score = 30.0 bits (66), Expect(4) = e-121
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -3
Query: 984 KERRKREMSKDYVDPPPAPLIE 919
+E + KDYVDPPPAP ++
Sbjct: 7 EEGHTQSHGKDYVDPPPAPFLD 28
Score = 23.5 bits (49), Expect(4) = e-121
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = -3
Query: 201 AAIKALGSFRSN 166
AA+KAL SFRSN
Sbjct: 267 AAVKALASFRSN 278
>gb|AAB36949.1| plasma membrane intrinsic protein PIP3 [Arabidopsis thaliana]
Length = 280
Score = 356 bits (913), Expect(4) = e-120
Identities = 173/197 (87%), Positives = 186/197 (93%)
Frame = -1
Query: 917 LAEIKLWSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAWSFGGMIFVL 738
+ E+K WSFYRALIAEFIATLLFL VTVATVIGHKKQTGPCDGVGLLGIAW+FGGMIFVL
Sbjct: 28 MGELKSWSFYRALIAEFIATLLFLIVTVATVIGHKKQTGPCDGVGLLGIAWAFGGMIFVL 87
Query: 737 VYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGLVKAFMKHPYNSL 558
VYCTAGISGGHINPAVTFGLFLA KVSL+RA+ YM+AQCLGAICGVG VKAFMK PYN+L
Sbjct: 88 VYCTAGISGGHINPAVTFGLFLAGKVSLVRALGYMIAQCLGAICGVGFVKAFMKTPYNTL 147
Query: 557 GGGANSVAHGYSKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFM 378
GGGAN+VA GYSKG+ALGAEIIGTFVLVYTVFSATDPKRSARDSH+PVLAPLPIGFAVFM
Sbjct: 148 GGGANTVADGYSKGTALGAEIIGTFVLVYTVFSATDPKRSARDSHIPVLAPLPIGFAVFM 207
Query: 377 VHLAPIPLTGTGLKPCQ 327
VHLA IP+TGTG+ P +
Sbjct: 208 VHLATIPITGTGINPAR 224
Score = 85.5 bits (210), Expect(4) = e-120
Identities = 37/53 (69%), Positives = 44/53 (82%)
Frame = -2
Query: 340 LSPARSFGAAVIFNHGQVWDDPWIFWVGPFVGALAAAAYHPYILRARGYQGTG 182
++PARSFGAAVI+N+ + WDD WIFWVGPF+GALAAAAYH YILRA + G
Sbjct: 220 INPARSFGAAVIYNNEKAWDDQWIFWVGPFLGALAAAAYHQYILRASAIKALG 272
Score = 33.1 bits (74), Expect(4) = e-120
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = -3
Query: 987 LKERRKREMSKDYVDPPPAPLIE 919
+ E K KDYVDPPPAPL++
Sbjct: 5 VSEEGKTHHGKDYVDPPPAPLLD 27
Score = 25.0 bits (53), Expect(4) = e-120
Identities = 11/12 (91%), Positives = 12/12 (99%)
Frame = -3
Query: 201 AAIKALGSFRSN 166
+AIKALGSFRSN
Sbjct: 266 SAIKALGSFRSN 277
>gb|AAB65787.1| plasma membrane intrinsic protein [Arabidopsis thaliana]
Length = 280
Score = 356 bits (913), Expect(4) = e-120
Identities = 173/197 (87%), Positives = 186/197 (93%)
Frame = -1
Query: 917 LAEIKLWSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAWSFGGMIFVL 738
+ E+K WSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAW+FGGMIFVL
Sbjct: 28 MGELKSWSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAWAFGGMIFVL 87
Query: 737 VYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGLVKAFMKHPYNSL 558
VYCTAGISGGHINPAVTF LFLA KVSL+RA+ YM+AQCLGAICGVG VKAFMK PYN+L
Sbjct: 88 VYCTAGISGGHINPAVTFRLFLAGKVSLVRALGYMIAQCLGAICGVGFVKAFMKTPYNTL 147
Query: 557 GGGANSVAHGYSKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFM 378
GGGAN+VA GYSKG+ALGAEIIGTFVLVYTVFSATDPKRSARDSH+PVLAPLPIGFAVFM
Sbjct: 148 GGGANTVADGYSKGTALGAEIIGTFVLVYTVFSATDPKRSARDSHIPVLAPLPIGFAVFM 207
Query: 377 VHLAPIPLTGTGLKPCQ 327
VHLA IP+TGTG+ P +
Sbjct: 208 VHLATIPITGTGINPAR 224
Score = 85.5 bits (210), Expect(4) = e-120
Identities = 37/53 (69%), Positives = 44/53 (82%)
Frame = -2
Query: 340 LSPARSFGAAVIFNHGQVWDDPWIFWVGPFVGALAAAAYHPYILRARGYQGTG 182
++PARSFGAAVI+N+ + WDD WIFWVGPF+GALAAAAYH YILRA + G
Sbjct: 220 INPARSFGAAVIYNNEKAWDDQWIFWVGPFLGALAAAAYHQYILRASAIKALG 272
Score = 33.1 bits (74), Expect(4) = e-120
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = -3
Query: 987 LKERRKREMSKDYVDPPPAPLIE 919
+ E K KDYVDPPPAPL++
Sbjct: 5 VSEEGKTHHGKDYVDPPPAPLLD 27
Score = 25.0 bits (53), Expect(4) = e-120
Identities = 11/12 (91%), Positives = 12/12 (99%)
Frame = -3
Query: 201 AAIKALGSFRSN 166
+AIKALGSFRSN
Sbjct: 266 SAIKALGSFRSN 277
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,000,556,031
Number of Sequences: 1393205
Number of extensions: 26931733
Number of successful extensions: 276614
Number of sequences better than 10.0: 6932
Number of HSP's better than 10.0 without gapping: 128458
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220048
length of database: 448,689,247
effective HSP length: 124
effective length of database: 275,931,827
effective search space used: 56566024535
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
