Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001663A_C01 KMC001663A_c01
(637 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAC01237.1| RNA Binding Protein 45 [Nicotiana plumbaginifolia] 183 2e-45
pir||T04823 hypothetical protein F10M23.340 - Arabidopsis thalia... 173 2e-42
ref|NP_567764.1| RNA binding protein 45 (RBP45), putative; prote... 173 2e-42
gb|AAG59664.1|AC084319_22 putative RNA binding protein [Oryza sa... 167 1e-40
dbj|BAC56019.1| putative RNA Binding Protein 45 [Oryza sativa (j... 164 9e-40
>emb|CAC01237.1| RNA Binding Protein 45 [Nicotiana plumbaginifolia]
Length = 409
Score = 183 bits (464), Expect = 2e-45
Identities = 90/133 (67%), Positives = 103/133 (76%), Gaps = 2/133 (1%)
Frame = -2
Query: 636 NDPSHTTIFVGNLDPNVNDDHLRQVFSPYGELVHVKIPAGKRCGFVQFPDRSGAEEALRV 457
+DP++TTIFVG LDP V ++HLRQVFSPYGELVHVKI AGKRCGFVQF R+ AE+AL
Sbjct: 277 SDPNNTTIFVGGLDPTVAEEHLRQVFSPYGELVHVKIVAGKRCGFVQFGTRASAEQALSS 336
Query: 456 LNGTLLGGRNVRLSWGRSPSH*QAQPDSNQYPAAGG-YYGYGQGYENYGYAPSGQDPNA- 283
LNGT LGG+++RLSWGRSPS Q D Q+ +GG YYGYGQGYE YGYAP QDPN
Sbjct: 337 LNGTQLGGQSIRLSWGRSPS--SKQTDQTQWGGSGGAYYGYGQGYEAYGYAPPAQDPNMY 394
Query: 282 YGSYPGYCELPTP 244
YG+YPGY P
Sbjct: 395 YGNYPGYANYQQP 407
Score = 40.4 bits (93), Expect = 0.020
Identities = 23/77 (29%), Positives = 43/77 (54%), Gaps = 8/77 (10%)
Frame = -2
Query: 618 TIFVGNLDPNVNDDHLRQVFSPYGELVHVKIPAGKRCG------FVQFPDRSGAEEALRV 457
++++G+L +++++L F GELV K+ K+ G F++F + AE L+
Sbjct: 85 SLWIGDLQYWMDENYLSTCFYHTGELVSAKVIRNKQTGQSEGYGFLEFRSHAAAETILQT 144
Query: 456 LNGTLLGG--RNVRLSW 412
NGTL+ +N R++W
Sbjct: 145 YNGTLMPNVEQNFRMNW 161
Score = 38.5 bits (88), Expect = 0.075
Identities = 27/80 (33%), Positives = 40/80 (49%), Gaps = 7/80 (8%)
Frame = -2
Query: 636 NDPSHTTIFVGNLDPNVNDDHLRQVF-SPYGELVHVKIPA------GKRCGFVQFPDRSG 478
+D + TIFVG+L +V D L++ F S Y + K+ K GFV+F D S
Sbjct: 171 DDSAEHTIFVGDLAADVTDYILQETFKSVYSSVRGAKVVTDRITGRSKGYGFVKFADESE 230
Query: 477 AEEALRVLNGTLLGGRNVRL 418
A+ +NG L R +R+
Sbjct: 231 QLRAMTEMNGVLCSTRPMRI 250
>pir||T04823 hypothetical protein F10M23.340 - Arabidopsis thaliana
gi|4455223|emb|CAB36546.1| putative DNA binding protein
[Arabidopsis thaliana] gi|7269553|emb|CAB79555.1|
putative DNA binding protein [Arabidopsis thaliana]
Length = 427
Score = 173 bits (438), Expect = 2e-42
Identities = 84/126 (66%), Positives = 100/126 (78%), Gaps = 2/126 (1%)
Frame = -2
Query: 636 NDPSHTTIFVGNLDPNVNDDHLRQVFSPYGELVHVKIPAGKRCGFVQFPDRSGAEEALRV 457
+DP++TTIFVG +D +V +D L+ VF +GELVHVKIPAGKRCGFVQ+ +R+ AE+AL V
Sbjct: 273 SDPTNTTIFVGAVDQSVTEDDLKSVFGQFGELVHVKIPAGKRCGFVQYANRACAEQALSV 332
Query: 456 LNGTLLGGRNVRLSWGRSPSH*QAQPDSNQYPAAGGYYGY-GQGYENYGYAPSGQDPNA- 283
LNGT LGG+++RLSWGRSPS+ Q QPD QY GGYYGY QGYE YGYAP QDPNA
Sbjct: 333 LNGTQLGGQSIRLSWGRSPSNKQTQPDQAQYGGGGGYYGYPPQGYEAYGYAPPPQDPNAY 392
Query: 282 YGSYPG 265
YG Y G
Sbjct: 393 YGGYAG 398
Score = 38.5 bits (88), Expect = 0.075
Identities = 30/95 (31%), Positives = 41/95 (42%), Gaps = 7/95 (7%)
Frame = -2
Query: 630 PSHTTIFVGNLDPNVNDDHLRQVFSP-YGELVHVKIP------AGKRCGFVQFPDRSGAE 472
P HT +FVG+L P+V D L + F Y + K+ K GFV+F D S
Sbjct: 171 PEHT-VFVGDLAPDVTDHMLTETFKAVYSSVKGAKVVNDRTTGRSKGYGFVRFADESEQI 229
Query: 471 EALRVLNGTLLGGRNVRLSWGRSPSH*QAQPDSNQ 367
A+ +NG R +R + QP S Q
Sbjct: 230 RAMTEMNGQYCSSRPMRTGPAANKKPLTMQPASYQ 264
Score = 36.6 bits (83), Expect = 0.29
Identities = 21/79 (26%), Positives = 42/79 (52%), Gaps = 8/79 (10%)
Frame = -2
Query: 618 TIFVGNLDPNVNDDHLRQVFSPYGELVHVKIPAGKR------CGFVQFPDRSGAEEALRV 457
++++G+L P +++++L VF GE K+ K+ GF++F + + AE L+
Sbjct: 81 SLWIGDLQPWMDENYLMNVFGLTGEATAAKVIRNKQNGYSEGYGFIEFVNHATAERNLQT 140
Query: 456 LNGTLL--GGRNVRLSWGR 406
NG + + RL+W +
Sbjct: 141 YNGAPMPSSEQAFRLNWAQ 159
>ref|NP_567764.1| RNA binding protein 45 (RBP45), putative; protein id: At4g27000.1,
supported by cDNA: gi_13878152, supported by cDNA:
gi_15450813, supported by cDNA: gi_17104568, supported
by cDNA: gi_20259887 [Arabidopsis thaliana]
gi|13878153|gb|AAK44154.1|AF370339_1 putative DNA
binding protein [Arabidopsis thaliana]
gi|15450814|gb|AAK96678.1| putative DNA binding protein
[Arabidopsis thaliana] gi|17104569|gb|AAL34173.1|
putative DNA binding protein [Arabidopsis thaliana]
gi|20259888|gb|AAM13291.1| putative DNA binding protein
[Arabidopsis thaliana]
Length = 415
Score = 173 bits (438), Expect = 2e-42
Identities = 84/126 (66%), Positives = 100/126 (78%), Gaps = 2/126 (1%)
Frame = -2
Query: 636 NDPSHTTIFVGNLDPNVNDDHLRQVFSPYGELVHVKIPAGKRCGFVQFPDRSGAEEALRV 457
+DP++TTIFVG +D +V +D L+ VF +GELVHVKIPAGKRCGFVQ+ +R+ AE+AL V
Sbjct: 273 SDPTNTTIFVGAVDQSVTEDDLKSVFGQFGELVHVKIPAGKRCGFVQYANRACAEQALSV 332
Query: 456 LNGTLLGGRNVRLSWGRSPSH*QAQPDSNQYPAAGGYYGY-GQGYENYGYAPSGQDPNA- 283
LNGT LGG+++RLSWGRSPS+ Q QPD QY GGYYGY QGYE YGYAP QDPNA
Sbjct: 333 LNGTQLGGQSIRLSWGRSPSNKQTQPDQAQYGGGGGYYGYPPQGYEAYGYAPPPQDPNAY 392
Query: 282 YGSYPG 265
YG Y G
Sbjct: 393 YGGYAG 398
Score = 38.5 bits (88), Expect = 0.075
Identities = 30/95 (31%), Positives = 41/95 (42%), Gaps = 7/95 (7%)
Frame = -2
Query: 630 PSHTTIFVGNLDPNVNDDHLRQVFSP-YGELVHVKIP------AGKRCGFVQFPDRSGAE 472
P HT +FVG+L P+V D L + F Y + K+ K GFV+F D S
Sbjct: 171 PEHT-VFVGDLAPDVTDHMLTETFKAVYSSVKGAKVVNDRTTGRSKGYGFVRFADESEQI 229
Query: 471 EALRVLNGTLLGGRNVRLSWGRSPSH*QAQPDSNQ 367
A+ +NG R +R + QP S Q
Sbjct: 230 RAMTEMNGQYCSSRPMRTGPAANKKPLTMQPASYQ 264
Score = 36.6 bits (83), Expect = 0.29
Identities = 21/79 (26%), Positives = 42/79 (52%), Gaps = 8/79 (10%)
Frame = -2
Query: 618 TIFVGNLDPNVNDDHLRQVFSPYGELVHVKIPAGKR------CGFVQFPDRSGAEEALRV 457
++++G+L P +++++L VF GE K+ K+ GF++F + + AE L+
Sbjct: 81 SLWIGDLQPWMDENYLMNVFGLTGEATAAKVIRNKQNGYSEGYGFIEFVNHATAERNLQT 140
Query: 456 LNGTLL--GGRNVRLSWGR 406
NG + + RL+W +
Sbjct: 141 YNGAPMPSSEQAFRLNWAQ 159
>gb|AAG59664.1|AC084319_22 putative RNA binding protein [Oryza sativa]
Length = 402
Score = 167 bits (423), Expect = 1e-40
Identities = 82/135 (60%), Positives = 103/135 (75%), Gaps = 3/135 (2%)
Frame = -2
Query: 636 NDPSHTTIFVGNLDPNVNDDHLRQVFSPYGELVHVKIPAGKRCGFVQFPDRSGAEEALRV 457
+DP++TT+FVG LDP+V D+ L+Q FSPYGELV+VKIP GKRCGFVQ+ +R+ AEEA+R+
Sbjct: 263 SDPNNTTVFVGGLDPSVTDEVLKQAFSPYGELVYVKIPVGKRCGFVQYSNRASAEEAIRM 322
Query: 456 LNGTLLGGRNVRLSWGRSPSH*QAQPDSNQYPAAGGYYGY-GQGYENYGYAPSGQDP--N 286
LNG+ LGG+++RLSWGRSP + Q Q D NQ+ A GYYGY QGY+ YGY QDP
Sbjct: 323 LNGSQLGGQSIRLSWGRSPGNKQPQQDQNQWNA--GYYGYPPQGYDPYGYVRPPQDPAMY 380
Query: 285 AYGSYPGYCELPTPA 241
AY +YPGY PA
Sbjct: 381 AYAAYPGYGNYQQPA 395
Score = 35.8 bits (81), Expect = 0.49
Identities = 36/124 (29%), Positives = 50/124 (40%), Gaps = 7/124 (5%)
Frame = -2
Query: 636 NDPSHTTIFVGNLDPNVNDDHLRQVFSPYGELVH-VKIP------AGKRCGFVQFPDRSG 478
+D S TIFVG+L +V D L+ F + + V K+ K GFV+F D
Sbjct: 155 DDGSDYTIFVGDLASDVTDLILQDTFKAHYQSVKGAKVVFDRSTGRSKGYGFVKFGDLDE 214
Query: 477 AEEALRVLNGTLLGGRNVRLSWGRSPSH*QAQPDSNQYPAAGGYYGYGQGYENYGYAPSG 298
A+ +NG R +R+ P SN+ GG Y+N S
Sbjct: 215 QTRAMTEMNGQYCSSRPMRIG-----------PASNK-KNIGGQQQPSATYQNTQGTDSD 262
Query: 297 QDPN 286
DPN
Sbjct: 263 SDPN 266
>dbj|BAC56019.1| putative RNA Binding Protein 45 [Oryza sativa (japonica
cultivar-group)]
Length = 502
Score = 164 bits (415), Expect = 9e-40
Identities = 83/129 (64%), Positives = 102/129 (78%), Gaps = 4/129 (3%)
Frame = -2
Query: 636 NDPSHTTIFVGNLDPNVNDDHLRQVFSPYGELVHVKIPAGKRCGFVQFPDRSGAEEALRV 457
NDP++TTIFVG LD NVN+DHL+QVF+PYGE+ +VKIP GKRCGFVQF RS AEEA+RV
Sbjct: 327 NDPNNTTIFVGGLDSNVNEDHLKQVFTPYGEIGYVKIPLGKRCGFVQFTSRSSAEEAIRV 386
Query: 456 LNGTLLGGRNVRLSWGRSPSH*QA-QPDSNQYPAAGGYYGYGQGYENYGY-APSGQDPNA 283
LNG+ +GG+ VRLSWGR+P + QA Q D+NQ+ G YYGY QGY++ Y AP+ QDP+A
Sbjct: 387 LNGSQIGGQQVRLSWGRTPQNKQAPQQDANQW--NGNYYGYQQGYDSSYYGAPNAQDPSA 444
Query: 282 --YGSYPGY 262
Y Y GY
Sbjct: 445 QNYYGYSGY 453
Score = 38.1 bits (87), Expect = 0.098
Identities = 34/112 (30%), Positives = 49/112 (43%), Gaps = 8/112 (7%)
Frame = -2
Query: 630 PSHTTIFVGNLDPNVNDDHLRQVFSPY------GELVHVKIPA-GKRCGFVQFPDRSGAE 472
P HT IFVG+L +V D L + F ++V K+ K GFV+F D +
Sbjct: 221 PEHT-IFVGDLASDVTDSMLEEAFKTSYPSVRGAKVVFDKVTGRSKGYGFVRFGDENEQT 279
Query: 471 EALRVLNGTLLGGRNVRLSWGRSPSH*QAQPDSNQYPAAGGYY-GYGQGYEN 319
A+ +NG L R +RL P+ + + Q + GY G EN
Sbjct: 280 RAMTEMNGATLSTRQMRL----GPAANKKNMGTQQTYSTNGYQSSQGNSLEN 327
Score = 33.9 bits (76), Expect = 1.9
Identities = 22/81 (27%), Positives = 43/81 (52%), Gaps = 9/81 (11%)
Frame = -2
Query: 618 TIFVGNLDPNVNDDHLRQVFSPYGELV-HVKIPAGKRCG------FVQFPDRSGAEEALR 460
++++G+L +++ +L F+P G+ V VK+ K+ G F++F + AE AL
Sbjct: 129 SLWIGDLQYWMDESYLSNAFAPMGQQVTSVKVIRNKQSGHSEGYGFIEFQSHAAAEYALA 188
Query: 459 VLNGTLL--GGRNVRLSWGRS 403
NG ++ + +L+W S
Sbjct: 189 NFNGRMMLNVDQLFKLNWASS 209
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 594,463,145
Number of Sequences: 1393205
Number of extensions: 14412963
Number of successful extensions: 49686
Number of sequences better than 10.0: 2029
Number of HSP's better than 10.0 without gapping: 42503
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 48208
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 26439068301
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
