Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC001488A_C07 KMC001488A_c07
(1411 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAK26763.1| plasma membrane integral protein ZmPIP2-7 [Zea mays] 216 5e-56
gb|AAL32127.1| aquaporin [Medicago truncatula] 201 6e-54
gb|AAF65845.1|AF255795_1 aquaporin 1 [Allium cepa] 196 7e-50
gb|AAK26758.1| plasma membrane integral protein ZmPIP2-1 [Zea mays] 192 1e-49
pir||T14599 plasma membrane major intrinsic protein 1 - beet gi|... 184 2e-49
>gb|AAK26763.1| plasma membrane integral protein ZmPIP2-7 [Zea mays]
Length = 287
Score = 216 bits (549), Expect(2) = 5e-56
Identities = 141/300 (47%), Positives = 164/300 (54%), Gaps = 25/300 (8%)
Frame = -1
Query: 1363 MAKDIEVTEQPGNTLPRTTVTHLQYRCSTREELTKWSLYRAVIAEFVATLLFLYITVLTI 1184
MAKD+E + G + +ELTKWSLYRA IAEF+ATLLFLYITVLTI
Sbjct: 1 MAKDVEQVTEQGEYSAKDYHDPPPAPLIDPDELTKWSLYRAAIAEFIATLLFLYITVLTI 60
Query: 1183 IGYKRQTDLGIPGNTECDGVGILGIAWA--------------LWWHDLRPCL-----LHR 1061
IGYKRQ+D IPGNTECDGVGILGIAWA + + P + L R
Sbjct: 61 IGYKRQSDTKIPGNTECDGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGR 120
Query: 1060 PVSQEGILTLL*HLGYSWDARCPW*GRYCTSLHSA*VQSVVLDLAKGFQKSFYNRYGGGA 881
VS L + A+C G C + LAKGFQKSFYNRYGGG
Sbjct: 121 KVSLVRALLYM-------IAQCA--GAICGA-----------GLAKGFQKSFYNRYGGGV 160
Query: 880 NTISDGYRQRAQLWVLRS*EPLFLSTLFSLLLILRGT------LGIPMFLYWHHFQLDLL 719
NT+SDGY + L + + T+FS R + P+ + + F + L
Sbjct: 161 NTVSDGYNKGTALGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLA 220
Query: 718 CSWFTWLQSLLLALGINPARSFGPAVIFNEDKVWDDQWVYWVGPFVGALVAAIYHQYILR 539
T GINPARSFGPAVIFN DK WDDQW+YWVGPFVGA VAAIYHQYILR
Sbjct: 221 TIPVT-------GTGINPARSFGPAVIFNNDKAWDDQWIYWVGPFVGAAVAAIYHQYILR 273
Score = 211 bits (537), Expect = 2e-53
Identities = 130/244 (53%), Positives = 147/244 (59%), Gaps = 19/244 (7%)
Frame = -3
Query: 1328 EYSAKDYSDPPPVPLFDP*GAHK-------------------VVSVQSCHSRVRSNPSLP 1206
EYSAKDY DPPP PL DP K + + + +S+ +P
Sbjct: 13 EYSAKDYHDPPPAPLIDPDELTKWSLYRAAIAEFIATLLFLYITVLTIIGYKRQSDTKIP 72
Query: 1205 LHHRVDHYWLQKTD*PRYSREH*V*WGWNFGYSLGPLVA*SSSLFTAPAGISGGHINPAV 1026
+ D + W FG + LV AGISGGHINPAV
Sbjct: 73 GNTECDGVGILGI-------------AWAFGGMIFILVY-------CTAGISGGHINPAV 112
Query: 1025 TFGLFVGRKVSLVRALLYIIAQCLGAICGAGLG*GLPKIILQQIWRWG*HNLRWLQAKGT 846
TFGLF+GRKVSLVRALLY+IAQC GAICGAGL G K + + G + + KGT
Sbjct: 113 TFGLFLGRKVSLVRALLYMIAQCAGAICGAGLAKGFQKSFYNR-YGGGVNTVSDGYNKGT 171
Query: 845 ALGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGH*P 666
ALGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTG P
Sbjct: 172 ALGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINP 231
Query: 665 SKEF 654
++ F
Sbjct: 232 ARSF 235
Score = 26.2 bits (56), Expect(2) = 5e-56
Identities = 12/12 (100%), Positives = 12/12 (100%)
Frame = -2
Query: 531 AIKALGSFRSNA 496
AIKALGSFRSNA
Sbjct: 276 AIKALGSFRSNA 287
>gb|AAL32127.1| aquaporin [Medicago truncatula]
Length = 287
Score = 201 bits (510), Expect = 3e-50
Identities = 137/297 (46%), Positives = 162/297 (54%), Gaps = 22/297 (7%)
Frame = -1
Query: 1363 MAKDIEVTEQPGNTLPRTTVTHLQYRCSTREELTKWSLYRAVIAEFVATLLFLYITVLTI 1184
M KD+E EQ G + +ELTKWSLYRAVIAEFVATLLFLYITVLTI
Sbjct: 1 MGKDVEGQEQGGEFSAKDYQDPPPAPLFDLDELTKWSLYRAVIAEFVATLLFLYITVLTI 60
Query: 1183 IGYKRQTDLGIPGNTECDGVGILGIAWALWWHDLRPCLLHRPVSQEGILTLL*H--LGYS 1010
IGY RQ+D I GNTECDGVG+LGIAWA G++ +L + G S
Sbjct: 61 IGYSRQSDTTIKGNTECDGVGVLGIAWAF----------------GGMIFILVYCTAGIS 104
Query: 1009 WDARCP------W*GRYCTSLHSA*VQSVVLDL--------AKGFQKSFYNRYGGGANTI 872
P + GR SL A + V L AKGFQ S+YNRY GG NT+
Sbjct: 105 GGHINPAVTFGLFVGRK-VSLIRAFLYIVAQCLGAICGAGLAKGFQTSYYNRYKGGVNTV 163
Query: 871 SDGYRQRAQLWVLRS*EPLFLSTLFSLLLILRGT------LGIPMFLYWHHFQLDLLCSW 710
SDGY + L + + T+FS R + P+ + + F + L
Sbjct: 164 SDGYSKGTALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIP 223
Query: 709 FTWLQSLLLALGINPARSFGPAVIFNEDKVWDDQWVYWVGPFVGALVAAIYHQYILR 539
T GINPARSFGPAVIFN +K WDDQW+YWVGPF+GA VAAIYHQYILR
Sbjct: 224 VT-------GTGINPARSFGPAVIFNNEKAWDDQWIYWVGPFIGAAVAAIYHQYILR 273
Score = 200 bits (508), Expect(3) = 6e-54
Identities = 104/136 (76%), Positives = 115/136 (84%)
Frame = -3
Query: 1061 AGISGGHINPAVTFGLFVGRKVSLVRALLYIIAQCLGAICGAGLG*GLPKIILQQIWRWG 882
AGISGGHINPAVTFGLFVGRKVSL+RA LYI+AQCLGAICGAGL G + ++ G
Sbjct: 101 AGISGGHINPAVTFGLFVGRKVSLIRAFLYIVAQCLGAICGAGLAKGFQTSYYNR-YKGG 159
Query: 881 *HNLRWLQAKGTALGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHL 702
+ + +KGTALGAEIIGTFVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHL
Sbjct: 160 VNTVSDGYSKGTALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHL 219
Query: 701 ATIPVTGTGH*PSKEF 654
ATIPVTGTG P++ F
Sbjct: 220 ATIPVTGTGINPARSF 235
Score = 29.6 bits (65), Expect(3) = 6e-54
Identities = 11/12 (91%), Positives = 12/12 (99%)
Frame = -2
Query: 1098 FGGMIFVLVYCT 1063
FGGMIF+LVYCT
Sbjct: 89 FGGMIFILVYCT 100
Score = 26.2 bits (56), Expect(3) = 6e-54
Identities = 12/12 (100%), Positives = 12/12 (100%)
Frame = -2
Query: 531 AIKALGSFRSNA 496
AIKALGSFRSNA
Sbjct: 276 AIKALGSFRSNA 287
>gb|AAF65845.1|AF255795_1 aquaporin 1 [Allium cepa]
Length = 291
Score = 196 bits (497), Expect(2) = 7e-50
Identities = 122/236 (51%), Positives = 146/236 (61%), Gaps = 11/236 (4%)
Frame = -3
Query: 1328 EYSAKDYSDPPPVPLFDP*GAHK-----------VVSVQSCHSRVRSNPSLPLHHRVDHY 1182
+++AKDY+DPPP PLFD K V ++ + V + + H+ D
Sbjct: 15 QFTAKDYTDPPPAPLFDMGELTKWSLYRAVIAEFVATLLFLYITVAT--VIGYSHQSDAT 72
Query: 1181 WLQKTD*PRYSREH*V*WGWNFGYSLGPLVA*SSSLFTAPAGISGGHINPAVTFGLFVGR 1002
TD S + W FG + LV AGISGGHINPAVTFGLF+ R
Sbjct: 73 IPGNTD-AHCSGVGVLGIAWAFGGMIFVLVY-------CTAGISGGHINPAVTFGLFLAR 124
Query: 1001 KVSLVRALLYIIAQCLGAICGAGLG*GLPKIILQQIWRWG*HNLRWLQAKGTALGAEIIG 822
KVSL+RALLY+IAQCLGAICG G + + G + L +KGT L AEIIG
Sbjct: 125 KVSLIRALLYMIAQCLGAICGVGFVKAFQSAYYSR-YGGGANTLNAGYSKGTGLAAEIIG 183
Query: 821 TFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGH*PSKEF 654
TFVLVYTVF+ATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIP+TGTG P++ F
Sbjct: 184 TFVLVYTVFAATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSF 239
Score = 176 bits (446), Expect = 8e-43
Identities = 115/272 (42%), Positives = 146/272 (53%), Gaps = 27/272 (9%)
Frame = -1
Query: 1270 ELTKWSLYRAVIAEFVATLLFLYITVLTIIGYKRQTDLGIPGNTE--CDGVGILGIAWA- 1100
ELTKWSLYRAVIAEFVATLLFLYITV T+IGY Q+D IPGNT+ C GVG+LGIAWA
Sbjct: 34 ELTKWSLYRAVIAEFVATLLFLYITVATVIGYSHQSDATIPGNTDAHCSGVGVLGIAWAF 93
Query: 1099 -------------LWWHDLRPCL-----LHRPVSQEGILTLL*HLGYSWDARCPW*GRYC 974
+ + P + L R VS I LL + A C
Sbjct: 94 GGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSL--IRALLYMIAQCLGAICG------ 145
Query: 973 TSLHSA*VQSVVLDLAKGFQKSFYNRYGGGANTISDGYRQRAQLWVLRS*EPLFLSTLFS 794
+ K FQ ++Y+RYGGGANT++ GY + L + + T+F+
Sbjct: 146 ------------VGFVKAFQSAYYSRYGGGANTLNAGYSKGTGLAAEIIGTFVLVYTVFA 193
Query: 793 LLLILRGT------LGIPMFLYWHHFQLDLLCSWFTWLQSLLLALGINPARSFGPAVIFN 632
R + P+ + + F + L T GINPARSFG AVI+N
Sbjct: 194 ATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPIT-------GTGINPARSFGAAVIYN 246
Query: 631 EDKVWDDQWVYWVGPFVGALVAAIYHQYILRA 536
+DK WDDQW++WVGPF+GA +AA YHQY+LRA
Sbjct: 247 KDKAWDDQWIFWVGPFIGAAIAAAYHQYVLRA 278
Score = 25.4 bits (54), Expect(2) = 7e-50
Identities = 11/13 (84%), Positives = 12/13 (91%)
Frame = -2
Query: 534 GAIKALGSFRSNA 496
GA+KALGSF SNA
Sbjct: 279 GAVKALGSFGSNA 291
>gb|AAK26758.1| plasma membrane integral protein ZmPIP2-1 [Zea mays]
Length = 290
Score = 192 bits (488), Expect(2) = 1e-49
Identities = 106/155 (68%), Positives = 117/155 (75%)
Frame = -3
Query: 1124 WNFGYSLGPLVA*SSSLFTAPAGISGGHINPAVTFGLFVGRKVSLVRALLYIIAQCLGAI 945
W FG + LV AGISGGHINPAVTFGLF+ RKVSLVRALLYI+AQCLGAI
Sbjct: 90 WAFGGMIFVLVY-------CTAGISGGHINPAVTFGLFLARKVSLVRALLYIVAQCLGAI 142
Query: 944 CGAGLG*GLPKIILQQIWRWG*HNLRWLQAKGTALGAEIIGTFVLVYTVFSATDPKRNAR 765
CG GL + + G ++L ++GT LGAEIIGTFVLVYTVFSATDPKRNAR
Sbjct: 143 CGVGLVKAFQSAYFDR-YGGGANSLASGYSRGTGLGAEIIGTFVLVYTVFSATDPKRNAR 201
Query: 764 DSHVPVLAPLPIGFAVFMVHLATIPVTGTGH*PSK 660
DSHVPVLAPLPIGFAVFMVHLATIPVTGTG P++
Sbjct: 202 DSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPAR 236
Score = 159 bits (401), Expect(2) = 2e-41
Identities = 109/271 (40%), Positives = 140/271 (51%), Gaps = 26/271 (9%)
Frame = -1
Query: 1270 ELTKWSLYRAVIAEFVATLLFLYITVLTIIGYKRQTDLGIPG-NTECDGVGILGIAWA-- 1100
EL WSLYRAVIAEF+ATLLFLYITV T+IGYK QTD G + C GVG+LGIAWA
Sbjct: 34 ELGSWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDASASGADAACGGVGVLGIAWAFG 93
Query: 1099 ------------LWWHDLRPCL-----LHRPVSQEGILTLL*HLGYSWDARCPW*GRYCT 971
+ + P + L R VS L + A+C G C
Sbjct: 94 GMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYI-------VAQCL--GAICG 144
Query: 970 SLHSA*VQSVVLDLAKGFQKSFYNRYGGGANTISDGYRQRAQLWVLRS*EPLFLSTLFSL 791
+ L K FQ ++++RYGGGAN+++ GY + L + + T+FS
Sbjct: 145 -----------VGLVKAFQSAYFDRYGGGANSLASGYSRGTGLGAEIIGTFVLVYTVFSA 193
Query: 790 LLILRGT------LGIPMFLYWHHFQLDLLCSWFTWLQSLLLALGINPARSFGPAVIFNE 629
R + P+ + + F + L T GINPARS G AVI+N+
Sbjct: 194 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVT-------GTGINPARSLGAAVIYNK 246
Query: 628 DKVWDDQWVYWVGPFVGALVAAIYHQYILRA 536
DK WDD W++WVGP VGA +AA YHQYILRA
Sbjct: 247 DKPWDDHWIFWVGPLVGAAIAAFYHQYILRA 277
Score = 33.9 bits (76), Expect(2) = 2e-41
Identities = 12/17 (70%), Positives = 15/17 (87%)
Frame = -3
Query: 1328 EYSAKDYSDPPPVPLFD 1278
E++AKDY+DPPP PL D
Sbjct: 15 EFAAKDYTDPPPAPLID 31
Score = 28.5 bits (62), Expect(2) = 1e-49
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = -2
Query: 534 GAIKALGSFRSNA 496
GAIKALGSFRSNA
Sbjct: 278 GAIKALGSFRSNA 290
>pir||T14599 plasma membrane major intrinsic protein 1 - beet
gi|1402833|gb|AAB67868.1| plasma membrane major intrinsic
protein 1 [Beta vulgaris]
Length = 288
Score = 184 bits (468), Expect(3) = 2e-49
Identities = 95/136 (69%), Positives = 108/136 (78%)
Frame = -3
Query: 1061 AGISGGHINPAVTFGLFVGRKVSLVRALLYIIAQCLGAICGAGLG*GLPKIILQQIWRWG 882
AGISGGHINPAVTFGLF+ RKVSL+RAL Y++AQCLGAICG G + + G
Sbjct: 102 AGISGGHINPAVTFGLFLARKVSLIRALAYMVAQCLGAICGVGFVKAFQSAYYDR-YGGG 160
Query: 881 *HNLRWLQAKGTALGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHL 702
+ + KGT LGAEIIGTFVLVYTVF+ATDPKR+ARDSHVPVLAPLPIGFAVFMVHL
Sbjct: 161 ANQMAHGYNKGTGLGAEIIGTFVLVYTVFAATDPKRSARDSHVPVLAPLPIGFAVFMVHL 220
Query: 701 ATIPVTGTGH*PSKEF 654
ATIP+TGTG P++ F
Sbjct: 221 ATIPITGTGINPARSF 236
Score = 158 bits (400), Expect(2) = 7e-41
Identities = 108/265 (40%), Positives = 138/265 (51%), Gaps = 19/265 (7%)
Frame = -1
Query: 1273 EELTKWSLYRAVIAEFVATLLFLYITVLTIIGYKRQTDLGIPGNTECDGVGILGIAWALW 1094
EEL KWS YRA+IAEFVATLLFLY+TVLT+IG+ R DL G C GVGILGIAWA
Sbjct: 33 EELKKWSFYRAIIAEFVATLLFLYVTVLTVIGHFRSADLE-NGGENCTGVGILGIAWAFG 91
Query: 1093 WHDLRPCLLHRPVSQEGI-------------LTLL*HLGYSWDARCPW*GRYCTSLHSA* 953
+S I ++L+ L Y A+C G C
Sbjct: 92 GMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRALAYMV-AQCL--GAICG------ 142
Query: 952 VQSVVLDLAKGFQKSFYNRYGGGANTISDGYRQRAQLWVLRS*EPLFLSTLFSLLLILRG 773
+ K FQ ++Y+RYGGGAN ++ GY + L + + T+F+ R
Sbjct: 143 -----VGFVKAFQSAYYDRYGGGANQMAHGYNKGTGLGAEIIGTFVLVYTVFAATDPKRS 197
Query: 772 TLG------IPMFLYWHHFQLDLLCSWFTWLQSLLLALGINPARSFGPAVIFNEDKVWDD 611
P+ + + F + L T GINPARSFG AVIFNE+K W++
Sbjct: 198 ARDSHVPVLAPLPIGFAVFMVHLATIPIT-------GTGINPARSFGAAVIFNEEKSWNE 250
Query: 610 QWVYWVGPFVGALVAAIYHQYILRA 536
W++WVGPFVGA +AA YHQYILRA
Sbjct: 251 HWIFWVGPFVGAAIAAFYHQYILRA 275
Score = 32.7 bits (73), Expect(2) = 7e-41
Identities = 12/17 (70%), Positives = 13/17 (75%)
Frame = -3
Query: 1328 EYSAKDYSDPPPVPLFD 1278
E+S KDY DPPP PL D
Sbjct: 15 EFSTKDYHDPPPAPLID 31
Score = 29.6 bits (65), Expect(3) = 2e-49
Identities = 11/12 (91%), Positives = 12/12 (99%)
Frame = -2
Query: 1098 FGGMIFVLVYCT 1063
FGGMIF+LVYCT
Sbjct: 90 FGGMIFILVYCT 101
Score = 26.6 bits (57), Expect(3) = 2e-49
Identities = 12/13 (92%), Positives = 13/13 (99%)
Frame = -2
Query: 534 GAIKALGSFRSNA 496
GAIKALGSFRS+A
Sbjct: 276 GAIKALGSFRSSA 288
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,340,830,652
Number of Sequences: 1393205
Number of extensions: 33529435
Number of successful extensions: 88693
Number of sequences better than 10.0: 778
Number of HSP's better than 10.0 without gapping: 79425
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 87448
length of database: 448,689,247
effective HSP length: 127
effective length of database: 271,752,212
effective search space used: 92939256504
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
