Asamizu E, Miura K, Kucho K, Inoue Y, Fukuzawa H, Ohyama K, Nakamura Y, Tabata S
Generation of Expressed Sequence Tags from low-CO2 and high-CO2 adapted cells of Chlamydomonas reinhardtii
DNA Research, 7, 305-307 (2000)

Figures and Tables

Table 1 Number of 5'-end ESTs generated from cDNA libraries of cells grown in low-CO2 and high-CO2 conditions
  Low-CO2 High-CO2 Standard
Number of ESTs
Normalized library
Size-selected library
 7113
5856		 7694
5756		 7310
4261
Total 12969 13450 11571
Number of non-redundant groupsa 4436 3566 3433
Number of specific groupsb 2665 1879 1753
Number of common groupsc   2117  

a) Number of groups occurred in each of the low-CO2, high-CO2 and standard conditions
b) Number of groups occurred only in the low-CO2, high-CO2 or standard conditions
c) Number of groups commonly occurred in two or three conditions among the low-CO2, high-CO2 and standard conditions


Table 2 Classification of 5'-end ESTs appeared in common or only in low-CO2 or high-CO2 EST populations by similarity search against the non-redundant protein database.
  Low-CO2 High-CO2 Common
Similarity Number of clones Number of non-redundant ESTs Number of clones Number of non-redundant ESTs Number of clones Number of non-redundant ESTs
Genes of known functiona) 648 384 363 269 14846 726
Hypothetical genesb) 139 91 64 50 831 105
No similarityc) 2647 2190 1740 1560 5141 1286
Total 3434 2665 2167 1879 20818 2117

a) Genes showed similarity to genes of known function,
b) Genes showed similarity to hypothetical genes that have no definition of function,
c) Genes showed no similarity


Table 3 Classification of the non-redundant EST groups appeared in common or only in low-CO2 or high-CO2 EST populations with similarity to known protein genes by their functional categories.
Functional categories Number of non-redundant groups
Common Low-CO2 High-CO2
Energy metabolism 142 43 39
Protein synthesis 126 34 31
Transport and binding proteins 52 22 9
Regulatory functions 51 28 17
Protein fate 46 28 23
Cellular processes 43 18 10
Amino-acid biosynthesis 32 17 8
General transcription 25 15 15
Fatty acid and phospholipid metabolism 24 5 3
Cellular structure, organization and biogenesis 22 36 22
Central intermediary metabolism 20 12 9
Biosynthesis of cofactors, prosthetic groups, and carriers 18 7 5
Signal transduction 18 23 13
Purines, pyrimidines, nucleosides, and nucleotides 11 8 7
Growth and development 6 10 2
DNA metabolism 5 8 4
Environmental response 4 3 1
Pathogen responses 2 2 0
Secondary metabolism 1 4 3
Other categories 26 7 12
Unclassified 52 54 36
Total 726 384 269


Figure 1 Size distribution of the inserts in analyzed cDNA clones from the normalized ( solid bars) and size-selected (gray bars) libraries of low-CO2 treated cells and normalized (solid bars with oblique lines) and size-selected (gray bars with oblique lines) libraries of high-CO2 treated cells.


Figure 2 The relationship among the non-redundant EST groups generated from cells from standard(S), low-CO2 (L) and high-CO2(H) growth conditions.

Chlamydomonas reinhardtii
Department of Plant Gene Research