Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC021550A_C01 KMC021550A_c01
(852 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAN84563.1| glutamine synthetase [Lotus japonicus] 548 e-159
gb|AAL67439.1| glutamine synthetase precursor [Lotus japonicus] 544 e-158
gb|AAF17703.1|AF031082_1 glutamine synthetase [Canavalia lineata] 505 e-146
sp|P08281|GLN2_PEA Glutamine synthetase leaf isozyme, chloroplas... 506 e-145
sp|Q9XQ94|GLN2_MEDSA Glutamine synthetase leaf isozyme, chloropl... 509 e-145
>gb|AAN84563.1| glutamine synthetase [Lotus japonicus]
Length = 430
Score = 548 bits (1412), Expect(2) = e-159
Identities = 260/261 (99%), Positives = 261/261 (99%)
Frame = +1
Query: 52 MWKQNKKVARASKFRVLAIKSDGSTINRLEGLLNLDVTPYTDKFIAEYIWIGGTGVDVRS 231
+WKQNKKVARASKFRVLAIKSDGSTINRLEGLLNLDVTPYTDKFIAEYIWIGGTGVDVRS
Sbjct: 34 LWKQNKKVARASKFRVLAIKSDGSTINRLEGLLNLDVTPYTDKFIAEYIWIGGTGVDVRS 93
Query: 232 KSRTISKPVSHPSELPKWNYDGSSTGQAPGDDSEVILYPQAIFRDPFRGGNNILVICDAY 411
KSRTISKPVSHPSELPKWNYDGSSTGQAPGDDSEVILYPQAIFRDPFRGGNNILVICDAY
Sbjct: 94 KSRTISKPVSHPSELPKWNYDGSSTGQAPGDDSEVILYPQAIFRDPFRGGNNILVICDAY 153
Query: 412 TPQGEPIPTNKRHRAAEIFSNPKVQAEIPWYGIEQEYTLLQTDVKWPLGWPVGGYPGPQG 591
TPQGEPIPTNKRHRAAEIFSNPKVQAEIPWYGIEQEYTLLQTDVKWPLGWPVGGYPGPQG
Sbjct: 154 TPQGEPIPTNKRHRAAEIFSNPKVQAEIPWYGIEQEYTLLQTDVKWPLGWPVGGYPGPQG 213
Query: 592 PYYCAAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIW 771
PYYCAAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIW
Sbjct: 214 PYYCAAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIW 273
Query: 772 ASRYLLERITEQAGVVLTLDP 834
ASRYLLERITEQAGVVLTLDP
Sbjct: 274 ASRYLLERITEQAGVVLTLDP 294
Score = 37.7 bits (86), Expect(2) = e-159
Identities = 16/25 (64%), Positives = 20/25 (80%)
Frame = +2
Query: 2 QNSPNASPITSNMWSSLCGNKTRKL 76
+ SPNASPITSNMWSSL + +K+
Sbjct: 17 KTSPNASPITSNMWSSLLWKQNKKV 41
>gb|AAL67439.1| glutamine synthetase precursor [Lotus japonicus]
Length = 430
Score = 544 bits (1402), Expect(2) = e-158
Identities = 258/261 (98%), Positives = 259/261 (98%)
Frame = +1
Query: 52 MWKQNKKVARASKFRVLAIKSDGSTINRLEGLLNLDVTPYTDKFIAEYIWIGGTGVDVRS 231
+WKQNKKVARASKFRVLAIKSDGSTINRLEGLLNLDVTPYTDKFIAEYIWIGGTGVDVRS
Sbjct: 34 LWKQNKKVARASKFRVLAIKSDGSTINRLEGLLNLDVTPYTDKFIAEYIWIGGTGVDVRS 93
Query: 232 KSRTISKPVSHPSELPKWNYDGSSTGQAPGDDSEVILYPQAIFRDPFRGGNNILVICDAY 411
KSRTISKPVSHPSELPKWNYDGSSTGQAPGDDSEVILYPQAIFRDPFRGGNNILVICDAY
Sbjct: 94 KSRTISKPVSHPSELPKWNYDGSSTGQAPGDDSEVILYPQAIFRDPFRGGNNILVICDAY 153
Query: 412 TPQGEPIPTNKRHRAAEIFSNPKVQAEIPWYGIEQEYTLLQTDVKWPLGWPVGGYPGPQG 591
TPQGEPIPTNKRHRAAEIFSNPKVQAEIPWYGIEQEYTLLQTDVKWPLGWPVGGYPGPQG
Sbjct: 154 TPQGEPIPTNKRHRAAEIFSNPKVQAEIPWYGIEQEYTLLQTDVKWPLGWPVGGYPGPQG 213
Query: 592 PYYCAAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIW 771
PYYCAAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGP VGIEAGDHIW
Sbjct: 214 PYYCAAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGPXVGIEAGDHIW 273
Query: 772 ASRYLLERITEQAGVVLTLDP 834
ASRYLLERIT QAGVVLTLDP
Sbjct: 274 ASRYLLERITXQAGVVLTLDP 294
Score = 37.7 bits (86), Expect(2) = e-158
Identities = 16/25 (64%), Positives = 20/25 (80%)
Frame = +2
Query: 2 QNSPNASPITSNMWSSLCGNKTRKL 76
+ SPNASPITSNMWSSL + +K+
Sbjct: 17 KTSPNASPITSNMWSSLLWKQNKKV 41
>gb|AAF17703.1|AF031082_1 glutamine synthetase [Canavalia lineata]
Length = 430
Score = 505 bits (1300), Expect(2) = e-146
Identities = 232/261 (88%), Positives = 250/261 (94%)
Frame = +1
Query: 52 MWKQNKKVARASKFRVLAIKSDGSTINRLEGLLNLDVTPYTDKFIAEYIWIGGTGVDVRS 231
+ KQNKK ++KFRVLAIKS+ TINRLE LL+LD+TPYTDK IAEYIW+GGTG+DVRS
Sbjct: 34 LMKQNKKATSSAKFRVLAIKSENGTINRLENLLDLDITPYTDKIIAEYIWVGGTGIDVRS 93
Query: 232 KSRTISKPVSHPSELPKWNYDGSSTGQAPGDDSEVILYPQAIFRDPFRGGNNILVICDAY 411
KSRTI++PV HPSELPKWNYDGSSTGQAPGDDSEVILYPQAIF+DPFRGGNNILVICDAY
Sbjct: 94 KSRTIARPVEHPSELPKWNYDGSSTGQAPGDDSEVILYPQAIFKDPFRGGNNILVICDAY 153
Query: 412 TPQGEPIPTNKRHRAAEIFSNPKVQAEIPWYGIEQEYTLLQTDVKWPLGWPVGGYPGPQG 591
TP GEPIPTNKRHRAAEIFSNPKVQAE+PWYGIEQEYTLLQT+V WPLGWPVGGYPGPQG
Sbjct: 154 TPAGEPIPTNKRHRAAEIFSNPKVQAEVPWYGIEQEYTLLQTNVNWPLGWPVGGYPGPQG 213
Query: 592 PYYCAAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIW 771
PYYC+AGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWE+QVGPSVGIEAGDHIW
Sbjct: 214 PYYCSAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEFQVGPSVGIEAGDHIW 273
Query: 772 ASRYLLERITEQAGVVLTLDP 834
A+RY+LERITEQAGVVL+LDP
Sbjct: 274 AARYILERITEQAGVVLSLDP 294
Score = 36.6 bits (83), Expect(2) = e-146
Identities = 15/24 (62%), Positives = 19/24 (78%)
Frame = +2
Query: 2 QNSPNASPITSNMWSSLCGNKTRK 73
+ SPNASP+TSNMWSSL + +K
Sbjct: 17 KTSPNASPVTSNMWSSLLMKQNKK 40
>sp|P08281|GLN2_PEA Glutamine synthetase leaf isozyme, chloroplast precursor
(Glutamate--ammonia ligase) (Chloroplast GS2)
gi|68597|pir||AJPMQ2 glutamate-ammonia ligase (EC
6.3.1.2) delta precursor, chloroplast - garden pea
gi|169059|gb|AAA33653.1| glutamine synthetase
(chloroplast GS2) (EC 6.3.1.2)
Length = 430
Score = 506 bits (1304), Expect(2) = e-145
Identities = 233/259 (89%), Positives = 250/259 (95%)
Frame = +1
Query: 58 KQNKKVARASKFRVLAIKSDGSTINRLEGLLNLDVTPYTDKFIAEYIWIGGTGVDVRSKS 237
KQ KKVA ++KFRV+A+ S+ TINR+E LLNLD+TP+TD IAEYIWIGGTG+DVRSKS
Sbjct: 36 KQTKKVAHSAKFRVMAVNSENGTINRVEDLLNLDITPFTDSIIAEYIWIGGTGIDVRSKS 95
Query: 238 RTISKPVSHPSELPKWNYDGSSTGQAPGDDSEVILYPQAIFRDPFRGGNNILVICDAYTP 417
RTISKPVSHPSE+PKWNYDGSSTGQAPG+DSEVILYPQAIF+DPFRGGNNILV+CDAYTP
Sbjct: 96 RTISKPVSHPSEVPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRGGNNILVVCDAYTP 155
Query: 418 QGEPIPTNKRHRAAEIFSNPKVQAEIPWYGIEQEYTLLQTDVKWPLGWPVGGYPGPQGPY 597
GEPIPTNKRHRAAEIFSNPKV+AEIPWYGIEQEYTLLQT+VKWPLGWPVGGYPGPQGPY
Sbjct: 156 AGEPIPTNKRHRAAEIFSNPKVEAEIPWYGIEQEYTLLQTNVKWPLGWPVGGYPGPQGPY 215
Query: 598 YCAAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIWAS 777
YCAAGADKSFGRDISDAHYKAC+YAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIWAS
Sbjct: 216 YCAAGADKSFGRDISDAHYKACIYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIWAS 275
Query: 778 RYLLERITEQAGVVLTLDP 834
RY+LERITEQAGVVLTLDP
Sbjct: 276 RYILERITEQAGVVLTLDP 294
Score = 32.3 bits (72), Expect(2) = e-145
Identities = 14/25 (56%), Positives = 19/25 (76%)
Frame = +2
Query: 2 QNSPNASPITSNMWSSLCGNKTRKL 76
+ SP A+PITS MWSSL +T+K+
Sbjct: 17 KTSPCATPITSKMWSSLVMKQTKKV 41
>sp|Q9XQ94|GLN2_MEDSA Glutamine synthetase leaf isozyme, chloroplast precursor
(Glutamate--ammonia ligase) (Chloroplast GS2)
gi|4731322|gb|AAD28443.1|AF124244_1 glutamine synthetase
precursor [Medicago sativa]
Length = 428
Score = 509 bits (1311), Expect(2) = e-145
Identities = 239/259 (92%), Positives = 251/259 (96%)
Frame = +1
Query: 58 KQNKKVARASKFRVLAIKSDGSTINRLEGLLNLDVTPYTDKFIAEYIWIGGTGVDVRSKS 237
KQNKKVAR++KFRV+AI S TINR+E LLNLD+TP+TD IAEYIWIGGTG+DVRSKS
Sbjct: 36 KQNKKVARSAKFRVMAINS--GTINRVEDLLNLDITPFTDSIIAEYIWIGGTGIDVRSKS 93
Query: 238 RTISKPVSHPSELPKWNYDGSSTGQAPGDDSEVILYPQAIFRDPFRGGNNILVICDAYTP 417
RTISKPV HPSELPKWNYDGSSTGQAPG+DSEVILYPQAIF+DPFRGGNNILVICDAYTP
Sbjct: 94 RTISKPVEHPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRGGNNILVICDAYTP 153
Query: 418 QGEPIPTNKRHRAAEIFSNPKVQAEIPWYGIEQEYTLLQTDVKWPLGWPVGGYPGPQGPY 597
QGEPIPTNKRH+AAEIFSNPKV+AEIPWYGIEQEYTLLQTDVKWPLGWPVGGYPGPQGPY
Sbjct: 154 QGEPIPTNKRHKAAEIFSNPKVEAEIPWYGIEQEYTLLQTDVKWPLGWPVGGYPGPQGPY 213
Query: 598 YCAAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIWAS 777
YCAAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIWAS
Sbjct: 214 YCAAGADKSFGRDISDAHYKACLYAGINISGTNGEVMPGQWEYQVGPSVGIEAGDHIWAS 273
Query: 778 RYLLERITEQAGVVLTLDP 834
RY+LERITEQAGVVLTLDP
Sbjct: 274 RYILERITEQAGVVLTLDP 292
Score = 28.9 bits (63), Expect(2) = e-145
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = +2
Query: 2 QNSPNASPITSNMWSSLCGNKTRKL 76
+ SP A+PI+S MWSSL + +K+
Sbjct: 17 KTSPLATPISSKMWSSLVMKQNKKV 41
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 783,795,937
Number of Sequences: 1393205
Number of extensions: 18341817
Number of successful extensions: 56095
Number of sequences better than 10.0: 473
Number of HSP's better than 10.0 without gapping: 52338
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 55600
length of database: 448,689,247
effective HSP length: 122
effective length of database: 278,718,237
effective search space used: 44873636157
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
