>KMC021501A_C01 KMC021501A_c01
gaagctgaAACTAATCCGAGCAAACTTTCATTTGTATTAATTCATGTGGACCTCCTCGAC
AAAGATAGGAAAGATAATTACAAAGAATAATAACAATAATTTGTCCTTGTGCTATCACGC
TCACTCTCAAAATTTGTACATCAATTAATGTTTGAAGCCAAGGTAGTGAGGAACATGAAG
AGCCTTGATCCAATCAAGGCCAGATCGAGGTCCAGCCTTAAGGAACTCACCGGCTGTGAA
TTGGGTAGCCTCAGCCCTGCTAATGACACCACGGTAACCCTTCCACTTGATCCTTCCTGC
AGTGTTGGCACCGGGTCCAGTGTTGGCATGCTCAGCATAGTACAAGGTGTCAAGGGCAAA
GCTTCCAGCCCATGGGAACCATCCCTCTGGATGAATGAAGTCACCCAAAGTAGATTCCAT
CACCACGGTCCTTGAGTACTCCTTCCATGGCCTTCCGAGGTAAGACTTGATCTTGAACCT
CACAGGGAACAATGCGGCTTCTGGGACAATCTCACAATCTTGGA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC021501A_C01 KMC021501A_c01
         (524 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

emb|CAC09467.1| putative pectin methylesterase [Oryza sativa (in...   166  2e-40
pir||T04359 pectin methylesterase-like protein - maize gi|223926...   152  3e-36
pir||T52331 pectinesterase (EC 3.1.1.11) [imported] - Salix gilg...   142  3e-33
pir||T16977 pectinesterase (EC 3.1.1.11) isoform 4 - curled-leav...   136  1e-31
pir||T16976 pectinesterase (EC 3.1.1.11) isoform 3 - curled-leav...   136  1e-31

>emb|CAC09467.1| putative pectin methylesterase [Oryza sativa (indica
           cultivar-group)]
          Length = 717

 Score =  166 bits (419), Expect = 2e-40
 Identities = 80/123 (65%), Positives = 91/123 (73%)
 Frame = -3

Query: 522 QDCEIVPEAALFPVRFKIKSYLGRPWKEYSRTVVMESTLGDFIHPEGWFPWAGSFALDTL 343
           QDC IVPE ALFPVR +I SYLGRPWKEY+RTVVMES +GDFI PEGW  W G   L TL
Sbjct: 599 QDCRIVPEQALFPVRLQIASYLGRPWKEYARTVVMESVIGDFIKPEGWSEWMGDVGLKTL 658

Query: 342 YYAEHANTGPGANTAGRIKWKGYRGVISRAEATQFTAGEFLKAGPRSGLDWIKALHVPHY 163
           YYAE+ANTGPGA T+ R+ W GYR VI +AEATQFTAG F+      GL W+K    P+ 
Sbjct: 659 YYAEYANTGPGAGTSKRVTWPGYR-VIGQAEATQFTAGVFI-----DGLTWLKNTATPNV 712

Query: 162 LGF 154
           +GF
Sbjct: 713 MGF 715

>pir||T04359 pectin methylesterase-like protein - maize
           gi|2239262|emb|CAA73733.1| pectin methylesterase-like
           protein [Zea mays]
          Length = 563

 Score =  152 bits (383), Expect = 3e-36
 Identities = 72/124 (58%), Positives = 86/124 (69%)
 Frame = -3

Query: 522 QDCEIVPEAALFPVRFKIKSYLGRPWKEYSRTVVMESTLGDFIHPEGWFPWAGSFALDTL 343
           Q+C +VP+  LFP RFKI SYLGRPWKE+SR V+MEST+ DF+ PEG+ PW G FAL TL
Sbjct: 445 QNCRLVPDQKLFPDRFKIPSYLGRPWKEFSRLVIMESTIADFVKPEGYMPWNGDFALKTL 504

Query: 342 YYAEHANTGPGANTAGRIKWKGYRGVISRAEATQFTAGEFLKAGPRSGLDWIKALHVPHY 163
           YYAE+ N GPGA T+ R+ W G+  VI R EA  FTAG F+      G  W+K    PH 
Sbjct: 505 YYAEYNNRGPGAGTSKRVNWPGFH-VIGRKEAEPFTAGPFI-----DGAMWLKYTGAPHI 558

Query: 162 LGFK 151
           LGFK
Sbjct: 559 LGFK 562

>pir||T52331 pectinesterase (EC 3.1.1.11) [imported] - Salix gilgiana
           gi|6714532|dbj|BAA89480.1| pectin methylesterase [Salix
           gilgiana]
          Length = 596

 Score =  142 bits (357), Expect = 3e-33
 Identities = 68/122 (55%), Positives = 82/122 (66%)
 Frame = -3

Query: 522 QDCEIVPEAALFPVRFKIKSYLGRPWKEYSRTVVMESTLGDFIHPEGWFPWAGSFALDTL 343
           Q C I P+  L PV+ KI+SYLGRPWKE+SRTV+M+ST+GDFIHP GW PW G F L TL
Sbjct: 481 QSCRIEPDKDLVPVKNKIRSYLGRPWKEFSRTVIMDSTIGDFIHPGGWLPWQGDFGLKTL 540

Query: 342 YYAEHANTGPGANTAGRIKWKGYRGVISRAEATQFTAGEFLKAGPRSGLDWIKALHVPHY 163
           YYAE++N G GA T  RIKW GY  +I + EA +FT   F +       DWI A   P +
Sbjct: 541 YYAEYSNKGGGAQTNARIKWPGYH-IIKKEEAMKFTIENFYQG------DWISASGSPVH 593

Query: 162 LG 157
           LG
Sbjct: 594 LG 595

>pir||T16977 pectinesterase (EC 3.1.1.11) isoform 4 - curled-leaved tobacco
           (fragment) gi|1279602|emb|CAA96436.1| pectin
           methylesterase [Nicotiana plumbaginifolia]
          Length = 274

 Score =  136 bits (343), Expect = 1e-31
 Identities = 63/122 (51%), Positives = 84/122 (68%)
 Frame = -3

Query: 522 QDCEIVPEAALFPVRFKIKSYLGRPWKEYSRTVVMESTLGDFIHPEGWFPWAGSFALDTL 343
           Q+C+I+P + L PV+  +K+YLGRPWK YSRTV M+S +GD I PEGW  W G FAL TL
Sbjct: 158 QNCDIIPSSDLAPVKGSVKTYLGRPWKAYSRTVFMQSNIGDHIDPEGWSVWDGDFALKTL 217

Query: 342 YYAEHANTGPGANTAGRIKWKGYRGVISRAEATQFTAGEFLKAGPRSGLDWIKALHVPHY 163
           YY E+ N GPGA T+ R+KW GY  ++S AEAT+FT G+ ++ G      W+K+  V + 
Sbjct: 218 YYGEYMNKGPGAGTSKRVKWPGYH-ILSAAEATKFTVGQLIQGGV-----WLKSTGVAYT 271

Query: 162 LG 157
            G
Sbjct: 272 EG 273

>pir||T16976 pectinesterase (EC 3.1.1.11) isoform 3 - curled-leaved tobacco
           (fragment) gi|1279600|emb|CAA96435.1| pectin
           methylesterase [Nicotiana plumbaginifolia]
          Length = 315

 Score =  136 bits (343), Expect = 1e-31
 Identities = 63/122 (51%), Positives = 84/122 (68%)
 Frame = -3

Query: 522 QDCEIVPEAALFPVRFKIKSYLGRPWKEYSRTVVMESTLGDFIHPEGWFPWAGSFALDTL 343
           Q+C+I+P + L PV+  +K+YLGRPWK YSRTV M+S +GD I PEGW  W G FAL TL
Sbjct: 199 QNCDIIPSSDLAPVKGSVKTYLGRPWKAYSRTVFMQSNIGDHIDPEGWSVWDGDFALKTL 258

Query: 342 YYAEHANTGPGANTAGRIKWKGYRGVISRAEATQFTAGEFLKAGPRSGLDWIKALHVPHY 163
           YY E+ N GPGA T+ R+KW GY  ++S AEAT+FT G+ ++ G      W+K+  V + 
Sbjct: 259 YYGEYMNKGPGAGTSKRVKWPGYH-ILSAAEATKFTVGQLIQGGV-----WLKSTGVAYT 312

Query: 162 LG 157
            G
Sbjct: 313 EG 314

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 472,128,345
Number of Sequences: 1393205
Number of extensions: 10599131
Number of successful extensions: 29434
Number of sequences better than 10.0: 217
Number of HSP's better than 10.0 without gapping: 27838
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29357
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17019769648
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFL004d12_f BP033596 1 512 2 MF062c04_f BP031575 9 430 3 MF060b05_f BP031447 13 482 4 MF090a09_f BP033003 15 524

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