Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC021362A_C01 KMC021362A_c01
(701 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_189154.1| cytochrome p450 family; protein id: At3g25180.1... 167 2e-40
pir||T07964 (S)-N-methylcoclaurine 3'-hydroxylase (EC 1.1.3.-) -... 166 2e-40
dbj|BAA35080.1| putative cytochrome P450 [Nicotiana tabacum] gi|... 151 1e-35
pir||T05942 cytochrome P450 82C1 - soybean gi|2739004|gb|AAB9459... 150 2e-35
ref|NP_194925.1| cytochrome p450 family; protein id: At4g31970.1... 149 5e-35
>ref|NP_189154.1| cytochrome p450 family; protein id: At3g25180.1 [Arabidopsis
thaliana] gi|9294175|dbj|BAB02077.1| cytochrome p450
[Arabidopsis thaliana]
Length = 515
Score = 167 bits (422), Expect = 2e-40
Identities = 82/142 (57%), Positives = 104/142 (72%), Gaps = 1/142 (0%)
Frame = -2
Query: 700 PPAPLTGIREATDDCHVAGYQVPKGTRLLINLWKLQRDPQIWSNPDEFQPERFLNAHEDI 521
PPAPLTGIREA +DC V GY+V KGTRLL+N+WKL RDP+IW +P F+PERF+ +
Sbjct: 376 PPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTFKPERFM--EDKS 433
Query: 520 SFQSQDFGFIPFSFGRRSCPGMNFGLQVLHLTLARLLQGFDICTKAG-VVDMIEGLGVAL 344
+ +F +IPF GRRSCPG+N GL+V+H LARLLQGF++ + +DM EG G+AL
Sbjct: 434 QCEKSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSDEPLDMAEGPGLAL 493
Query: 343 PKANALEVVLEPRLPLKLYESL 278
PK N +EVV+ PRL KLY L
Sbjct: 494 PKINPVEVVVMPRLDPKLYSLL 515
>pir||T07964 (S)-N-methylcoclaurine 3'-hydroxylase (EC 1.1.3.-) - California
poppy gi|3127031|gb|AAC39454.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 560
Score = 166 bits (421), Expect = 2e-40
Identities = 78/143 (54%), Positives = 102/143 (70%), Gaps = 4/143 (2%)
Frame = -2
Query: 700 PPAPLTGIREATDDCHVAGYQVPKGTRLLINLWKLQRDPQIWSNPDEFQPERFLNAHED- 524
P PL+ EA +DC+V GY + GTRLL+N+WKLQRDP++WSNP EF+PERFL+ +
Sbjct: 417 PAGPLSVPHEAIEDCNVGGYHIKTGTRLLVNIWKLQRDPRVWSNPSEFRPERFLDNQSNG 476
Query: 523 --ISFQSQDFGFIPFSFGRRSCPGMNFGLQVLHLTLARLLQGFDICT-KAGVVDMIEGLG 353
+ F+ Q F +IPF GRR CPG+NF +LH+TLARLLQ FD+ T + VDM EG G
Sbjct: 477 TLLDFRGQHFEYIPFGSGRRMCPGVNFATLILHMTLARLLQAFDLSTPSSSPVDMTEGSG 536
Query: 352 VALPKANALEVVLEPRLPLKLYE 284
+ +PK L+V+L PRLPL LY+
Sbjct: 537 LTMPKVTPLKVLLTPRLPLPLYD 559
>dbj|BAA35080.1| putative cytochrome P450 [Nicotiana tabacum]
gi|14423329|gb|AAK62347.1| elicitor-inducible cytochrome
P450 [Nicotiana tabacum]
Length = 519
Score = 151 bits (381), Expect = 1e-35
Identities = 72/140 (51%), Positives = 95/140 (67%), Gaps = 1/140 (0%)
Frame = -2
Query: 700 PPAPLTGIREATDDCHVAGYQVPKGTRLLINLWKLQRDPQIWSNPDEFQPERFLNAHEDI 521
PPAPL+ + DC V GY +PKGT LL N+ KLQRDPQIW +PD F PERFL + I
Sbjct: 379 PPAPLSVQHLSVKDCVVNGYHIPKGTALLTNIMKLQRDPQIWVDPDTFDPERFLTTNAAI 438
Query: 520 SFQSQDFGFIPFSFGRRSCPGMNFGLQVLHLTLARLLQGFDICTKAG-VVDMIEGLGVAL 344
++ Q + IPF GRR+CP MN+ LQV HL++A L+QGF+ T +DM +G+G+ L
Sbjct: 439 DYRGQHYELIPFGSGRRACPAMNYSLQVEHLSIAHLIQGFNFATTTNEPLDMKQGVGLTL 498
Query: 343 PKANALEVVLEPRLPLKLYE 284
PK +EV++ PRLP LY+
Sbjct: 499 PKKTDVEVLITPRLPPTLYQ 518
>pir||T05942 cytochrome P450 82C1 - soybean gi|2739004|gb|AAB94590.1| CYP82C1p
[Glycine max]
Length = 532
Score = 150 bits (379), Expect = 2e-35
Identities = 67/142 (47%), Positives = 101/142 (70%), Gaps = 2/142 (1%)
Frame = -2
Query: 700 PPAPLTGIREATDDCHVAG-YQVPKGTRLLINLWKLQRDPQIWSNPDEFQPERFLNAHED 524
PP+PL +R A +DC +G Y +P GTRL++N WK+ RD ++WS+P +F+P RFL +H+D
Sbjct: 391 PPSPLITLRAAMEDCTFSGGYHIPAGTRLMVNAWKIHRDGRVWSDPHDFKPGRFLTSHKD 450
Query: 523 ISFQSQDFGFIPFSFGRRSCPGMNFGLQVLHLTLARLLQGFDICTKAG-VVDMIEGLGVA 347
+ + Q++ +PF GRR+CPG + L+V+HLT+ARLL F++ + + VVDM E +G+
Sbjct: 451 VDVKGQNYELVPFGSGRRACPGASLALRVVHLTMARLLHSFNVASPSNQVVDMTESIGLT 510
Query: 346 LPKANALEVVLEPRLPLKLYES 281
KA LE++L PRL KLYE+
Sbjct: 511 NLKATPLEILLTPRLDTKLYEN 532
>ref|NP_194925.1| cytochrome p450 family; protein id: At4g31970.1 [Arabidopsis
thaliana] gi|7430659|pir||T04648 cytochrome P450
F10N7.220 - Arabidopsis thaliana
gi|2827638|emb|CAA16592.1| Cytochrome P450-like protein
[Arabidopsis thaliana] gi|7270101|emb|CAB79915.1|
Cytochrome P450-like protein [Arabidopsis thaliana]
Length = 523
Score = 149 bits (375), Expect = 5e-35
Identities = 71/140 (50%), Positives = 97/140 (68%), Gaps = 2/140 (1%)
Frame = -2
Query: 700 PPAPLTGIREATDDCHVAGYQVPKGTRLLINLWKLQRDPQIWSNPDEFQPERFLNAH-ED 524
P PL G REA +DC VAGY V +GTR+L+N+WK+QRDP+++ P+EF+PERF+ ++
Sbjct: 383 PAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRPERFITGEAKE 442
Query: 523 ISFQSQDFGFIPFSFGRRSCPGMNFGLQVLHLTLARLLQGFDICTKAGV-VDMIEGLGVA 347
+ Q+F +PF GRRSCPG + +QVLHL LAR LQ FD+ T + VDM E G+
Sbjct: 443 FDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMDMPVDMTESPGLT 502
Query: 346 LPKANALEVVLEPRLPLKLY 287
+PKA LE+++ PRL LY
Sbjct: 503 IPKATPLEILISPRLKEGLY 522
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 602,514,356
Number of Sequences: 1393205
Number of extensions: 13182383
Number of successful extensions: 59456
Number of sequences better than 10.0: 2991
Number of HSP's better than 10.0 without gapping: 34126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 48291
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 32250355128
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
