Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC021266A_C01 KMC021266A_c01
(621 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAC10115.1| contains EST AU056950(S21011)~similar to Arabido... 177 8e-44
pdb|1VFY|A Chain A, Phosphatidylinositol-3-Phosphate Binding Fyv... 74 2e-12
sp|P40343|VP27_YEAST Vacuolar protein sorting-associated protein... 74 2e-12
ref|NP_014403.1| hydrophilic protein; has cysteine rich putative... 74 2e-12
gb|EAA08780.1| agCP7758 [Anopheles gambiae str. PEST] 71 1e-11
>dbj|BAC10115.1| contains EST AU056950(S21011)~similar to Arabidopsis thaliana
chromosome 2,full-Length cDNA At2g26210~unknown protein
[Oryza sativa (japonica cultivar-group)]
gi|22831172|dbj|BAC16032.1| contains EST
AU056950(S21011)~similar to Arabidopsis thaliana
chromosome 2,full-Length cDNA At2g26210~unknown protein
[Oryza sativa (japonica cultivar-group)]
Length = 269
Score = 177 bits (450), Expect = 8e-44
Identities = 92/202 (45%), Positives = 129/202 (63%), Gaps = 9/202 (4%)
Frame = +2
Query: 41 PPPFQEAARCVVCSCSFNTFRRRHHCRSCGRTLCNEHSSDQMALPQFGLYSNVRVCADCF 220
PPPFQE+A C VC C+F TFRRRHHCR+CGRTLC+EHSS MALPQ+G+Y++VRVC DCF
Sbjct: 6 PPPFQESAHCDVCRCTFTTFRRRHHCRNCGRTLCHEHSSYHMALPQYGIYTDVRVCYDCF 65
Query: 221 NNSRSQ-EVPHASSDG-VNNITDTISKLDIDGNVDSKTLPSADNNPVSGIKECKCGMPLC 394
N S S+ V +A S G V++ D+ S L++ + S + ++ + + ECKCGMPLC
Sbjct: 66 NKSSSRGGVGNAGSPGSVSSAADSFSGLNLGEDDASSPMKNSAFHSAPAVIECKCGMPLC 125
Query: 395 ICEAPAPSSDALPQQKKISPAMTASSNPKPRKTDTVS-------KNRSSSSTNKFSSPFN 553
ICEAP P + SNP+P+K+ T K S++S++ SS N
Sbjct: 126 ICEAPKPE------------PVPVKSNPRPKKSSTNQQSAESSVKKASATSSSNSSSFLN 173
Query: 554 LGHVTNVASARPQTDYEVSGEG 619
LG ++N + + ++Y+V+GEG
Sbjct: 174 LGLMSNDTNDKGPSEYDVTGEG 195
>pdb|1VFY|A Chain A, Phosphatidylinositol-3-Phosphate Binding Fyve Domain Of
Vps27p Protein From Saccharomyces Cerevisiae
Length = 73
Score = 73.6 bits (179), Expect = 2e-12
Identities = 29/65 (44%), Positives = 40/65 (60%)
Frame = +2
Query: 32 STEPPPFQEAARCVVCSCSFNTFRRRHHCRSCGRTLCNEHSSDQMALPQFGLYSNVRVCA 211
S P + ++ C++CS F+ R+HHCRSCG C EHSS+ + LP G+Y VRVC
Sbjct: 2 SKTPADWIDSDACMICSKKFSLLNRKHHCRSCGGVFCQEHSSNSIPLPDLGIYEPVRVCD 61
Query: 212 DCFNN 226
CF +
Sbjct: 62 SCFED 66
>sp|P40343|VP27_YEAST Vacuolar protein sorting-associated protein VPS27
gi|785067|gb|AAA96002.1| Vps27p
Length = 622
Score = 73.6 bits (179), Expect = 2e-12
Identities = 29/65 (44%), Positives = 40/65 (60%)
Frame = +2
Query: 32 STEPPPFQEAARCVVCSCSFNTFRRRHHCRSCGRTLCNEHSSDQMALPQFGLYSNVRVCA 211
S P + ++ C++CS F+ R+HHCRSCG C EHSS+ + LP G+Y VRVC
Sbjct: 164 SKTPADWIDSDACMICSKKFSLLNRKHHCRSCGGVFCQEHSSNSIPLPDLGIYEPVRVCD 223
Query: 212 DCFNN 226
CF +
Sbjct: 224 SCFED 228
>ref|NP_014403.1| hydrophilic protein; has cysteine rich putative zinc finger
esential for function; Vps27p [Saccharomyces cerevisiae]
gi|626464|pir||S45129 VPS27 protein - yeast
(Saccharomyces cerevisiae) gi|496723|emb|CAA54574.1|
N2038 [Saccharomyces cerevisiae]
gi|1302478|emb|CAA96282.1| ORF YNR006w [Saccharomyces
cerevisiae]
Length = 622
Score = 73.6 bits (179), Expect = 2e-12
Identities = 29/65 (44%), Positives = 40/65 (60%)
Frame = +2
Query: 32 STEPPPFQEAARCVVCSCSFNTFRRRHHCRSCGRTLCNEHSSDQMALPQFGLYSNVRVCA 211
S P + ++ C++CS F+ R+HHCRSCG C EHSS+ + LP G+Y VRVC
Sbjct: 164 SKTPADWIDSDACMICSKKFSLLNRKHHCRSCGGVFCQEHSSNSIPLPDLGIYEPVRVCD 223
Query: 212 DCFNN 226
CF +
Sbjct: 224 SCFED 228
>gb|EAA08780.1| agCP7758 [Anopheles gambiae str. PEST]
Length = 687
Score = 70.9 bits (172), Expect = 1e-11
Identities = 32/67 (47%), Positives = 41/67 (60%), Gaps = 3/67 (4%)
Frame = +2
Query: 29 MSTEPP---PFQEAARCVVCSCSFNTFRRRHHCRSCGRTLCNEHSSDQMALPQFGLYSNV 199
M EPP P +A RC+ C+ +F FRRRHHCR+CG C S+ LP++GL V
Sbjct: 615 MPEEPPRWIPDCDAPRCMACASAFTPFRRRHHCRNCGGVFCGVCSNLSKPLPKYGLTKAV 674
Query: 200 RVCADCF 220
RVC DC+
Sbjct: 675 RVCRDCY 681
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 526,569,102
Number of Sequences: 1393205
Number of extensions: 11429074
Number of successful extensions: 39799
Number of sequences better than 10.0: 383
Number of HSP's better than 10.0 without gapping: 36515
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 39432
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25017613016
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
