Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC020560A_C01 KMC020560A_c01
(577 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_176205.1| metalloproteinase, putative; protein id: At1g59... 93 3e-18
sp|P29136|MEP1_SOYBN Metalloendoproteinase 1 precursor (SMEP1) g... 79 5e-14
ref|NP_177174.1| matrix metalloproteinase, putative; protein id:... 74 1e-12
ref|NP_173824.1| putative metalloproteinase; protein id: At1g241... 70 2e-11
gb|AAM62476.1| proteinase like protein [Arabidopsis thaliana] 60 2e-08
>ref|NP_176205.1| metalloproteinase, putative; protein id: At1g59970.1 [Arabidopsis
thaliana] gi|4249376|gb|AAD14473.1|AAD14473 Strong
similarity to gi|2829864 F3I6.6 zinc metalloproteinase
homolog from Arabidopsis thaliana BAC gb|AC002396. EST
gb|Z26412 comes from this gene
Length = 360
Score = 92.8 bits (229), Expect = 3e-18
Identities = 55/165 (33%), Positives = 84/165 (50%), Gaps = 29/165 (17%)
Frame = +2
Query: 167 IGRIPSKYTSTVKVIPPQNTIK----------------VGDSVKGLENIKKYLDALGYIK 298
+ I +K+ + V IPP + +G+++ GL +K+Y GYI
Sbjct: 15 VNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQYFRRFGYI- 73
Query: 299 DLVLALNSNDTDKFTEDLKPAIMEYQKNFNLPVTGVIDQNLYNTLIQPRCGVPDF----T 466
N TD F + L+ AI YQKNFNL VTG +D + +++PRCG PD +
Sbjct: 74 ----TTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCGNPDLIDGVS 129
Query: 467 TLNVG----TTPAF-----KPWWKSEKKELSYAFHPQNNVSDDIR 574
+N G TT + KP W K++L+YAF PQNN++D+++
Sbjct: 130 EMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVK 174
>sp|P29136|MEP1_SOYBN Metalloendoproteinase 1 precursor (SMEP1) gi|7488703|pir||T08836
probable metalloproteinase (EC 3.4.24.-) - soybean
gi|1679656|gb|AAB26959.1| metalloproteinase [Glycine
max]
Length = 305
Score = 78.6 bits (192), Expect = 5e-14
Identities = 46/129 (35%), Positives = 64/129 (48%), Gaps = 11/129 (8%)
Frame = +2
Query: 224 TIKVGDSVKGLENIKKYLDALGYIKDLVLALNSNDTDKFTEDLKPAIMEYQKNFNLPVTG 403
T G + KGL N+K Y LGYI + + D F + L AI YQKN+NL VTG
Sbjct: 44 TYHPGQNYKGLSNVKNYFHHLGYIPNA-----PHFDDNFDDTLVSAIKTYQKNYNLNVTG 98
Query: 404 VIDQNLYNTLIQPRCGVPDFTTLNVGTTPAF-----------KPWWKSEKKELSYAFHPQ 550
D N ++ PRCGVPD +N T +F P W++ +L+YAF P+
Sbjct: 99 KFDINTLKQIMTPRCGVPDI-IINTNKTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPE 157
Query: 551 NNVSDDIRT 577
+ D ++
Sbjct: 158 PRLDDTFKS 166
>ref|NP_177174.1| matrix metalloproteinase, putative; protein id: At1g70170.1
[Arabidopsis thaliana] gi|25406033|pir||E96724
hypothetical protein F20P5.11 [imported] - Arabidopsis
thaliana gi|2194124|gb|AAB61099.1| Similar to Glycine
metalloendoproteinase (gb|U63725). [Arabidopsis
thaliana]
Length = 378
Score = 73.9 bits (180), Expect = 1e-12
Identities = 44/138 (31%), Positives = 68/138 (48%), Gaps = 24/138 (17%)
Frame = +2
Query: 236 GDSVKGLENIKKYLDALGYIKDLVLALNSNDTDKFTEDLKPAIMEYQKNFNLPVTGVIDQ 415
G +V GL IKKY GYI + + N TD F + LK A+ YQ NFNL VTG +D
Sbjct: 53 GQNVDGLYRIKKYFQRFGYIPE---TFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDA 109
Query: 416 NLYNTLIQPRCGVPDF----TTLNVGTTPAF--------------------KPWWKSEKK 523
++ PRCG PD + ++ G F +P W ++
Sbjct: 110 LTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRR 169
Query: 524 ELSYAFHPQNNVSDDIRT 577
+L+YAF P+N +++++++
Sbjct: 170 DLTYAFDPKNPLTEEVKS 187
>ref|NP_173824.1| putative metalloproteinase; protein id: At1g24140.1 [Arabidopsis
thaliana] gi|7488435|pir||T00643 zinc metalloproteinase
homolog F3I6.6 - Arabidopsis thaliana
gi|2829864|gb|AAC00572.1| similar to zinc
metalloproteinases [Arabidopsis thaliana]
Length = 377
Score = 69.7 bits (169), Expect = 2e-11
Identities = 43/139 (30%), Positives = 67/139 (47%), Gaps = 25/139 (17%)
Frame = +2
Query: 236 GDSVKGLENIKKYLDALGYIKDLVLALNSNDTDKFTEDLKPAIMEYQKNFNLPVTGVIDQ 415
G GL +K+Y GYI + L+ N TD F + LK A+ YQ+NF L VTGV+D+
Sbjct: 48 GKKYDGLYMLKQYFQHFGYITET--NLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDE 105
Query: 416 NLYNTLIQPRCGVPD--------------FTTLNVGTTPAF-----------KPWWKSEK 520
++ PRCG PD F G F +P W +
Sbjct: 106 LTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNR 165
Query: 521 KELSYAFHPQNNVSDDIRT 577
++L+YAF P+N +++++++
Sbjct: 166 RDLTYAFDPRNALTEEVKS 184
>gb|AAM62476.1| proteinase like protein [Arabidopsis thaliana]
Length = 364
Score = 60.5 bits (145), Expect = 2e-08
Identities = 42/130 (32%), Positives = 67/130 (51%), Gaps = 13/130 (10%)
Frame = +2
Query: 227 IKVGDSVKGLENIKKYLDALGYIKDLVLALNSNDTDKFTEDLKPAIMEYQKNFNLPVTGV 406
+++G V G+ +K+YL GY+KD + +D F L+ AI YQ+N LP+TG
Sbjct: 61 VQIGSHVSGVSELKRYLHRFGYVKDG----SEIFSDVFDGPLESAISLYQENLGLPITGR 116
Query: 407 IDQNLYNTLIQPRCGVPD-FTTLN---VGTTPAF-----KPWWKSEKKELSYAFHPQNNV 559
+D + + PRCGV D T+N + TT + KP W + L+YA + +
Sbjct: 117 LDTSTVTLMSLPRCGVXDTHMTINNDFLHTTAHYTYFNGKPKW--NRDTLTYAISKTHKL 174
Query: 560 ----SDDIRT 577
S+D++T
Sbjct: 175 DYLTSEDVKT 184
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 511,852,138
Number of Sequences: 1393205
Number of extensions: 11958295
Number of successful extensions: 35247
Number of sequences better than 10.0: 259
Number of HSP's better than 10.0 without gapping: 34034
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35192
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 21530810025
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
