Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC020495A_C01 KMC020495A_c01
(439 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_187947.1| pectinesterase (pectin methylesterase) family; ... 177 2e-44
ref|NP_175953.1| pectinesterase (pectin methylesterase) family; ... 176 8e-44
ref|NP_187948.1| pectinesterase (pectin methylesterase) family; ... 175 1e-43
pir||S24950 pollen-specific protein Bp10 (clone Bp 1002) - rape ... 174 3e-43
pir||S24951 pollen-specific protein Bp10 (clone Bp 1003) - rape ... 174 3e-43
>ref|NP_187947.1| pectinesterase (pectin methylesterase) family; protein id:
At3g13390.1 [Arabidopsis thaliana]
gi|9280289|dbj|BAB01744.1| l-ascorbate oxidase;
pectinesterase-like protein; pollen-specific
protein-like [Arabidopsis thaliana]
gi|28393140|gb|AAO42003.1| putative pectinesterase
(pectin methylesterase) family protein [Arabidopsis
thaliana] gi|28827494|gb|AAO50591.1| putative
pectinesterase (pectin methylesterase) family protein
[Arabidopsis thaliana]
Length = 554
Score = 177 bits (450), Expect = 2e-44
Identities = 80/105 (76%), Positives = 92/105 (87%)
Frame = -3
Query: 437 GYSFFLVAVEPGKWTPEKRKNYNLLDTVSRHTVQVFPQSWAAIMLTFDNAGMWNLRSEHA 258
GYSFF VAVEPG WTPEKRKNYNLLD VSRHTVQV+P+ WAAI+LTFDN GMWN+RSE++
Sbjct: 447 GYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAAILLTFDNCGMWNVRSENS 506
Query: 257 ENRYLGQQLYISVLSPERSLRDEYNILDSQLLCGIVKDMPRPQPY 123
E RYLGQQLY SVLSPE+SLRDEYN+ ++ L CG+VK P+P PY
Sbjct: 507 ERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGTPKPNPY 551
>ref|NP_175953.1| pectinesterase (pectin methylesterase) family; protein id:
At1g55570.1, supported by cDNA: gi_13605852 [Arabidopsis
thaliana] gi|25285552|pir||D96598 hypothetical protein
T5A14.1 [imported] - Arabidopsis thaliana
gi|4204257|gb|AAD10638.1| putative pollen specific
protein [Arabidopsis thaliana]
gi|13605853|gb|AAK32912.1|AF367325_1 At1g55570/T5A14_1
[Arabidopsis thaliana] gi|22137174|gb|AAM91432.1|
At1g55570/T5A14_1 [Arabidopsis thaliana]
Length = 555
Score = 176 bits (445), Expect = 8e-44
Identities = 80/105 (76%), Positives = 91/105 (86%)
Frame = -3
Query: 437 GYSFFLVAVEPGKWTPEKRKNYNLLDTVSRHTVQVFPQSWAAIMLTFDNAGMWNLRSEHA 258
GYSFF VAVEPG WTPEKRKNYNLLD VSRHTVQV+P+ WAAI+LTFDN GMWN+RSE+A
Sbjct: 448 GYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAAILLTFDNCGMWNIRSENA 507
Query: 257 ENRYLGQQLYISVLSPERSLRDEYNILDSQLLCGIVKDMPRPQPY 123
E RYLGQQLY SVLSPE+SLRDEYN+ ++ L CG+VK P+ PY
Sbjct: 508 ERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGKPKVNPY 552
>ref|NP_187948.1| pectinesterase (pectin methylesterase) family; protein id:
At3g13400.1, supported by cDNA: gi_15912262, supported
by cDNA: gi_18176448, supported by cDNA: gi_20465920
[Arabidopsis thaliana] gi|9280290|dbj|BAB01745.1| BNH
protein; pectinesterase-like protein; pollen-secific
protein-like [Arabidopsis thaliana]
gi|15912263|gb|AAL08265.1| AT3g13400/MRP15_3
[Arabidopsis thaliana] gi|18176449|gb|AAL60046.1|
putative pollen specific protein [Arabidopsis thaliana]
gi|20465921|gb|AAM20113.1| putative pollen-specific
protein [Arabidopsis thaliana]
Length = 551
Score = 175 bits (444), Expect = 1e-43
Identities = 81/105 (77%), Positives = 92/105 (87%)
Frame = -3
Query: 437 GYSFFLVAVEPGKWTPEKRKNYNLLDTVSRHTVQVFPQSWAAIMLTFDNAGMWNLRSEHA 258
GYSFF VA EPG+WTPEKR NYNLLD VSRHTVQV+P+SW+AI+LTFDNAGMWN+RSE+
Sbjct: 445 GYSFFAVASEPGRWTPEKRNNYNLLDAVSRHTVQVYPKSWSAILLTFDNAGMWNIRSENW 504
Query: 257 ENRYLGQQLYISVLSPERSLRDEYNILDSQLLCGIVKDMPRPQPY 123
E RYLGQQLY+SVLSPE+SLRDEYNI + LCGIVK +P P PY
Sbjct: 505 ERRYLGQQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGLPLPTPY 549
>pir||S24950 pollen-specific protein Bp10 (clone Bp 1002) - rape
gi|17795|emb|CAA47177.1| Bplo [Brassica napus]
Length = 555
Score = 174 bits (440), Expect = 3e-43
Identities = 79/106 (74%), Positives = 93/106 (87%), Gaps = 1/106 (0%)
Frame = -3
Query: 437 GYSFFLVAVEPGKWTPEKRKNYNLLDTVSRHTVQVFPQSWAAIMLTFDNAGMWNLRSEHA 258
GYSFF VAVEPG WTPEKRKNYNLLD VSRHT+QV+P+ WAAI+LTFDN GMWN+RSE++
Sbjct: 447 GYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTIQVYPKCWAAILLTFDNCGMWNIRSENS 506
Query: 257 ENRYLGQQLYISVLSPERSLRDEYNILDSQLLCGIVKDMPRP-QPY 123
E RYLGQQLY SVLSPE+SLRDEYN+ ++ L CG+VK+ P+P PY
Sbjct: 507 ERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKNTPKPVNPY 552
>pir||S24951 pollen-specific protein Bp10 (clone Bp 1003) - rape
gi|17797|emb|CAA47178.1| Bplo [Brassica napus]
Length = 555
Score = 174 bits (440), Expect = 3e-43
Identities = 79/106 (74%), Positives = 93/106 (87%), Gaps = 1/106 (0%)
Frame = -3
Query: 437 GYSFFLVAVEPGKWTPEKRKNYNLLDTVSRHTVQVFPQSWAAIMLTFDNAGMWNLRSEHA 258
GYSFF VAVEPG WTPEKRKNYNLLD VSRHTVQV+P+ WAAI+LTFDN GMWN+RSE++
Sbjct: 447 GYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAAILLTFDNCGMWNIRSENS 506
Query: 257 ENRYLGQQLYISVLSPERSLRDEYNILDSQLLCGIVKDMPRP-QPY 123
E RYLGQQLY S+LSPE+SLRDEYN+ ++ L CG+VK+ P+P PY
Sbjct: 507 ERRYLGQQLYASILSPEKSLRDEYNMPETSLQCGLVKNTPKPVNPY 552
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 384,590,813
Number of Sequences: 1393205
Number of extensions: 7897960
Number of successful extensions: 18896
Number of sequences better than 10.0: 164
Number of HSP's better than 10.0 without gapping: 18336
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18887
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 6722674608
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
