Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC020474A_C01 KMC020474A_c01
(571 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAB88668.1| histone H2B [Cicer arietinum] 182 3e-45
gb|AAM66958.1| histone H2B [Arabidopsis thaliana] 181 4e-45
ref|NP_172258.1| histone H2B; protein id: At1g07790.1, supported... 181 4e-45
pir||T06390 histone H2B-3 - tomato (fragment) gi|3021485|emb|CAA... 180 1e-44
sp|O49118|H2B_CAPAN Histone H2B (CaH2B) gi|7439757|pir||T08063 h... 178 4e-44
>emb|CAB88668.1| histone H2B [Cicer arietinum]
Length = 139
Score = 182 bits (462), Expect = 3e-45
Identities = 91/133 (68%), Positives = 108/133 (80%)
Frame = -3
Query: 530 EEASGRRNPRRRKPRRRRNPRPRRRSRRKEAARRRRRRARRASRPYKIYIFKVLKQVHPD 351
E+ + P K + P+ ++ ++ + ++++R +++ YKIYIFKVLKQVHPD
Sbjct: 7 EKKPAEKKPAAEKAPAEKKPKAEKKISKEGTSDKKKKRVKKSVETYKIYIFKVLKQVHPD 66
Query: 350 IGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLGLPGELAKHAV 171
IGISSKAMGIMNSFINDIFEKLAQE+SRLARYNKKPTITSREIQTAVRL LPGELAKHAV
Sbjct: 67 IGISSKAMGIMNSFINDIFEKLAQEASRLARYNKKPTITSREIQTAVRLVLPGELAKHAV 126
Query: 170 SEGTKAVTKFTSS 132
SEGTKAVTKFTSS
Sbjct: 127 SEGTKAVTKFTSS 139
Score = 70.9 bits (172), Expect = 1e-11
Identities = 34/47 (72%), Positives = 40/47 (84%)
Frame = -2
Query: 537 RPRRSQRQKKPAAEKAPAEKKPKAEKKIAKEGGSEKKKKKSKKSVET 397
+ + +KKPAAEKAPAEKKPKAEKKI+KEG S+KKKK+ KKSVET
Sbjct: 5 KAEKKPAEKKPAAEKAPAEKKPKAEKKISKEGTSDKKKKRVKKSVET 51
Score = 31.2 bits (69), Expect = 9.5
Identities = 18/50 (36%), Positives = 28/50 (56%), Gaps = 5/50 (10%)
Frame = -1
Query: 550 MAPAKAEKKP-----AAEETRGGESPGGEETQGREEDREGRRQREEEEEE 416
MAPAKAEKKP AAE+ + P E+ +E + +++R ++ E
Sbjct: 1 MAPAKAEKKPAEKKPAAEKAPAEKKPKAEKKISKEGTSDKKKKRVKKSVE 50
>gb|AAM66958.1| histone H2B [Arabidopsis thaliana]
Length = 148
Score = 181 bits (460), Expect = 4e-45
Identities = 95/133 (71%), Positives = 108/133 (80%)
Frame = -3
Query: 530 EEASGRRNPRRRKPRRRRNPRPRRRSRRKEAARRRRRRARRASRPYKIYIFKVLKQVHPD 351
E + + P +KP+ + P KEA ++++R+++ YKIYIFKVLKQVHPD
Sbjct: 22 ENKAAEKAPAEKKPKAGKKLPP------KEAGDKKKKRSKKNVETYKIYIFKVLKQVHPD 75
Query: 350 IGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLGLPGELAKHAV 171
IGISSKAMGIMNSFINDIFEKLAQESS+LARYNKKPTITSREIQTAVRL LPGELAKHAV
Sbjct: 76 IGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLPGELAKHAV 135
Query: 170 SEGTKAVTKFTSS 132
SEGTKAVTKFTSS
Sbjct: 136 SEGTKAVTKFTSS 148
Score = 53.1 bits (126), Expect = 2e-06
Identities = 25/40 (62%), Positives = 32/40 (79%)
Frame = -2
Query: 516 QKKPAAEKAPAEKKPKAEKKIAKEGGSEKKKKKSKKSVET 397
++ AAEKAPAEKKPKA KK+ + +KKKK+SKK+VET
Sbjct: 21 EENKAAEKAPAEKKPKAGKKLPPKEAGDKKKKRSKKNVET 60
>ref|NP_172258.1| histone H2B; protein id: At1g07790.1, supported by cDNA: 8711.,
supported by cDNA: gi_12083299, supported by cDNA:
gi_17979525 [Arabidopsis thaliana]
gi|25294271|pir||D86213 hypothetical protein [imported]
- Arabidopsis thaliana
gi|8439888|gb|AAF75074.1|AC007583_10 Strong similarity
to histone H2B like protein from Arabidopsis thaliana
gb|Y07745. ESTs gb|R83948 and gb|T42349 come from this
gene gi|12083300|gb|AAG48809.1|AF332446_1 putative
histone H2B protein [Arabidopsis thaliana]
gi|17979526|gb|AAL50098.1| At1g07790/F24B9_10
[Arabidopsis thaliana] gi|22137246|gb|AAM91468.1|
At1g07790/F24B9_10 [Arabidopsis thaliana]
Length = 148
Score = 181 bits (460), Expect = 4e-45
Identities = 95/133 (71%), Positives = 108/133 (80%)
Frame = -3
Query: 530 EEASGRRNPRRRKPRRRRNPRPRRRSRRKEAARRRRRRARRASRPYKIYIFKVLKQVHPD 351
E + + P +KP+ + P KEA ++++R+++ YKIYIFKVLKQVHPD
Sbjct: 22 ENKAAEKAPAEKKPKAGKKLPP------KEAGDKKKKRSKKNVETYKIYIFKVLKQVHPD 75
Query: 350 IGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLGLPGELAKHAV 171
IGISSKAMGIMNSFINDIFEKLAQESS+LARYNKKPTITSREIQTAVRL LPGELAKHAV
Sbjct: 76 IGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLPGELAKHAV 135
Query: 170 SEGTKAVTKFTSS 132
SEGTKAVTKFTSS
Sbjct: 136 SEGTKAVTKFTSS 148
Score = 53.1 bits (126), Expect = 2e-06
Identities = 25/40 (62%), Positives = 32/40 (79%)
Frame = -2
Query: 516 QKKPAAEKAPAEKKPKAEKKIAKEGGSEKKKKKSKKSVET 397
++ AAEKAPAEKKPKA KK+ + +KKKK+SKK+VET
Sbjct: 21 EENKAAEKAPAEKKPKAGKKLPPKEAGDKKKKRSKKNVET 60
Score = 33.5 bits (75), Expect = 1.9
Identities = 19/38 (50%), Positives = 23/38 (60%), Gaps = 5/38 (13%)
Frame = -2
Query: 507 PAAEKAPAEKKPKAEK-----KIAKEGGSEKKKKKSKK 409
P AEK PAEKK AE+ K A++ +EKK K KK
Sbjct: 3 PRAEKKPAEKKTAAERPVEENKAAEKAPAEKKPKAGKK 40
>pir||T06390 histone H2B-3 - tomato (fragment) gi|3021485|emb|CAA12231.1|
histone H2B-3 [Lycopersicon esculentum]
Length = 137
Score = 180 bits (457), Expect = 1e-44
Identities = 95/137 (69%), Positives = 110/137 (79%), Gaps = 1/137 (0%)
Frame = -3
Query: 539 KGREEASGRRNPRRRKPRRRRNPRPRRRSRRKEAAR-RRRRRARRASRPYKIYIFKVLKQ 363
K E+ + P K + P+ ++ + AA ++++R+++A YKIYIFKVLKQ
Sbjct: 1 KPAEKKPAEKTPVAEKAPAEKKPKAGKKLPKDAAAGDKKKKRSKKAVETYKIYIFKVLKQ 60
Query: 362 VHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLGLPGELA 183
VHPDIGISSKAMGIMNSFINDIFEKLAQE+SRLARYNKKPTITSREIQTAVRL LPGELA
Sbjct: 61 VHPDIGISSKAMGIMNSFINDIFEKLAQEASRLARYNKKPTITSREIQTAVRLVLPGELA 120
Query: 182 KHAVSEGTKAVTKFTSS 132
KHAVSEGTKAVTKFTSS
Sbjct: 121 KHAVSEGTKAVTKFTSS 137
>sp|O49118|H2B_CAPAN Histone H2B (CaH2B) gi|7439757|pir||T08063 histone H2B - pepper
gi|2746719|gb|AAB94923.1| histone H2B [Capsicum annuum]
Length = 145
Score = 178 bits (452), Expect = 4e-44
Identities = 96/134 (71%), Positives = 108/134 (79%), Gaps = 1/134 (0%)
Frame = -3
Query: 530 EEASGRRNPRRRKPRR-RRNPRPRRRSRRKEAARRRRRRARRASRPYKIYIFKVLKQVHP 354
EE P +KP+ ++ P+ R +K ++RA+++ YKIYIFKVLKQVHP
Sbjct: 18 EEKKAEEVPAEKKPKAGKKLPKDAGRPDKK------KKRAKKSIETYKIYIFKVLKQVHP 71
Query: 353 DIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLGLPGELAKHA 174
DIGISSK+MGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRL LPGELAKHA
Sbjct: 72 DIGISSKSMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHA 131
Query: 173 VSEGTKAVTKFTSS 132
VSEGTKAVTKFTSS
Sbjct: 132 VSEGTKAVTKFTSS 145
Score = 50.4 bits (119), Expect = 2e-05
Identities = 24/49 (48%), Positives = 36/49 (72%), Gaps = 1/49 (2%)
Frame = -2
Query: 540 QRPRRSQRQKKPAAEKAPAEKKPKAEKKIAKEGG-SEKKKKKSKKSVET 397
++P + ++ AE+ PAEKKPKA KK+ K+ G +KKKK++KKS+ET
Sbjct: 9 KKPAEKKPVEEKKAEEVPAEKKPKAGKKLPKDAGRPDKKKKRAKKSIET 57
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 480,955,517
Number of Sequences: 1393205
Number of extensions: 10889970
Number of successful extensions: 189943
Number of sequences better than 10.0: 3461
Number of HSP's better than 10.0 without gapping: 81210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144426
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20956655091
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
