>KMC019972A_C01 KMC019972A_c01
ggagagactctccacaaatatctgcatcagcatggaatcttagccctgcatcaccaccat
caatcctcatcccacagtcaAGAAAAACAAAACAAAACATTATTATTATTATTATTCCCA
CACTGGTTTTATTAAATAAAACTATATAAAACTATATAATGTATAGAAGTGAATGTCTCT
GGATTGTTCATGAGGCTAAGAAGAGAAGGGAAGAGTCTTTTTCCTCTGCTCTCTTCACAG
CCCAAAACAAAATGCCAACTTGTGTGTCCATGTTGACCAAATCCAACTTGTCATTCATCC
AACTTATATAATTTTTATATATATATCAGATACATATAGATATATATAATCATAGACTAG
TGTTATTGTTATTGTGATTGTGTCTGGGTTTAATCTTGGTTCCTCTGTTTTCTTTGATGG
TTTGGTTGTGAGAAGAGAAGATTGTACTTTGGTTAGGGTTTGTTCATTCAATTCGCCGAC
AAGAAACTTCGGTTTCATATTTGATTGGTACTTTGGTTGTGGAAGTTAAGATTGTTGTTG
TTGTTGATTTTGGGAAGAATGGCTAATGGAGGAGACAAAAGCAATGATTTCTATGCTGTT
CTGGGCTTGAATAAGGAGTGCACTGAATCAGAGTTGAGGAATGCATATAAGAAACTTGCA
CTGAAATGGCACCCAGATCGTTGTTCAGCTTCAGGGAATTTGAAGTTTGTGGAAGAAGCT
AAGAAGAAATTCCAGTCAATTCAAGAAGCTTATTCTGTTTTATCTGATGCGAACAAAAGA
TTAATGTACGACATAGGAGTTTATGATAGTGATGATGACG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC019972A_C01 KMC019972A_c01
         (820 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_188036.1| hypothetical protein; protein id: At3g14200.1, ...   124  2e-27
gb|AAM63624.1| DnaJ protein, putative [Arabidopsis thaliana]           96  8e-19
ref|NP_564717.1| DnaJ protein, putative; protein id: At1g56300.1...    95  1e-18
pir||G96604 probable DnaJ protein [imported] - Arabidopsis thali...    95  1e-18
pir||T09295 probable heat shock protein EMB1 - white spruce gi|1...    91  3e-17

>ref|NP_188036.1| hypothetical protein; protein id: At3g14200.1, supported by cDNA:
           gi_20268706 [Arabidopsis thaliana]
           gi|9294648|dbj|BAB02987.1| gene_id:MAG2.19~unknown
           protein [Arabidopsis thaliana]
           gi|20268707|gb|AAM14057.1| unknown protein [Arabidopsis
           thaliana] gi|21689885|gb|AAM67503.1| unknown protein
           [Arabidopsis thaliana]
          Length = 230

 Score =  124 bits (310), Expect = 2e-27
 Identities = 57/86 (66%), Positives = 74/86 (85%)
 Frame = +1

Query: 562 ANGGDKSNDFYAVLGLNKECTESELRNAYKKLALKWHPDRCSASGNLKFVEEAKKKFQSI 741
           +N    + + YAVLGL KEC+++ELR+AYKKLAL+WHPDRCS+   ++FVEEAKKKFQ+I
Sbjct: 4   SNSEKINENLYAVLGLKKECSKTELRSAYKKLALRWHPDRCSS---MEFVEEAKKKFQAI 60

Query: 742 QEAYSVLSDANKRLMYDIGVYDSDDD 819
           QEAYSVLSD+NKR +YD+G Y++DDD
Sbjct: 61  QEAYSVLSDSNKRFLYDVGAYNTDDD 86

>gb|AAM63624.1| DnaJ protein, putative [Arabidopsis thaliana]
          Length = 156

 Score = 95.5 bits (236), Expect = 8e-19
 Identities = 43/84 (51%), Positives = 62/84 (73%)
 Frame = +1

Query: 568 GGDKSNDFYAVLGLNKECTESELRNAYKKLALKWHPDRCSASGNLKFVEEAKKKFQSIQE 747
           G +  + +Y VLG+ K+ + S++R AY+KLA+KWHPDR + +  +    EAK++FQ IQE
Sbjct: 7   GSNVRSSYYTVLGIRKDASVSDIRTAYRKLAMKWHPDRYARNPGV--AGEAKRRFQQIQE 64

Query: 748 AYSVLSDANKRLMYDIGVYDSDDD 819
           AYSVL+D NKR MYD+G+YD  +D
Sbjct: 65  AYSVLNDENKRSMYDVGLYDPHED 88

>ref|NP_564717.1| DnaJ protein, putative; protein id: At1g56300.1, supported by cDNA:
           25796. [Arabidopsis thaliana] gi|22135874|gb|AAM91519.1|
           DnaJ protein, putative [Arabidopsis thaliana]
          Length = 156

 Score = 95.1 bits (235), Expect = 1e-18
 Identities = 42/84 (50%), Positives = 62/84 (73%)
 Frame = +1

Query: 568 GGDKSNDFYAVLGLNKECTESELRNAYKKLALKWHPDRCSASGNLKFVEEAKKKFQSIQE 747
           G +  + +Y +LG+ K+ + S++R AY+KLA+KWHPDR + +  +    EAK++FQ IQE
Sbjct: 7   GSNVRSSYYTILGIRKDASVSDIRTAYRKLAMKWHPDRYARNPGV--AGEAKRRFQQIQE 64

Query: 748 AYSVLSDANKRLMYDIGVYDSDDD 819
           AYSVL+D NKR MYD+G+YD  +D
Sbjct: 65  AYSVLNDENKRSMYDVGLYDPHED 88

>pir||G96604 probable DnaJ protein [imported] - Arabidopsis thaliana
           gi|12321759|gb|AAG50919.1|AC069159_20 DnaJ protein,
           putative [Arabidopsis thaliana]
          Length = 97

 Score = 95.1 bits (235), Expect = 1e-18
 Identities = 42/84 (50%), Positives = 62/84 (73%)
 Frame = +1

Query: 568 GGDKSNDFYAVLGLNKECTESELRNAYKKLALKWHPDRCSASGNLKFVEEAKKKFQSIQE 747
           G +  + +Y +LG+ K+ + S++R AY+KLA+KWHPDR + +  +    EAK++FQ IQE
Sbjct: 7   GSNVRSSYYTILGIRKDASVSDIRTAYRKLAMKWHPDRYARNPGV--AGEAKRRFQQIQE 64

Query: 748 AYSVLSDANKRLMYDIGVYDSDDD 819
           AYSVL+D NKR MYD+G+YD  +D
Sbjct: 65  AYSVLNDENKRSMYDVGLYDPHED 88

>pir||T09295 probable heat shock protein EMB1 - white spruce
           gi|1350548|gb|AAB01572.1| heat shock-like protein [Picea
           glauca]
          Length = 158

 Score = 90.5 bits (223), Expect = 3e-17
 Identities = 41/77 (53%), Positives = 58/77 (75%)
 Frame = +1

Query: 589 FYAVLGLNKECTESELRNAYKKLALKWHPDRCSASGNLKFVEEAKKKFQSIQEAYSVLSD 768
           FY++LG+NK+ + +E+R+AY+KLA+KWHPD+ S   +    E AK +FQ IQEAYSVLSD
Sbjct: 5   FYSILGVNKDSSSAEIRSAYRKLAMKWHPDKWSTDPSSS--ETAKLRFQQIQEAYSVLSD 62

Query: 769 ANKRLMYDIGVYDSDDD 819
             KR +YD G+Y+  +D
Sbjct: 63  DTKRALYDAGMYEPSED 79

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 704,612,758
Number of Sequences: 1393205
Number of extensions: 15738361
Number of successful extensions: 76146
Number of sequences better than 10.0: 1245
Number of HSP's better than 10.0 without gapping: 47565
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 70580
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 42296827742
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFB061c05_f BP038418 1 472 2 MF085g06_f BP032790 297 820

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