Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC019872A_C01 KMC019872A_c01
(578 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_563764.1| cysteine proteinase; protein id: At1g06260.1 [A... 142 2e-33
ref|NP_567983.1| cysteine protease XCP1; protein id: At4g35350.1... 136 2e-31
gb|AAL60580.1|AF454958_1 senescence-associated cysteine protease... 132 3e-30
gb|AAM73806.1|AF448505_1 cysteine proteinase [Brassica napus] gi... 131 7e-30
gb|AAD53012.1|AF089849_1 senescence-specific cysteine protease [... 130 9e-30
>ref|NP_563764.1| cysteine proteinase; protein id: At1g06260.1 [Arabidopsis thaliana]
gi|25289993|pir||D86198 cysteine proteinase (EC
3.4.22.-) [similarity] - Arabidopsis thaliana
gi|8844131|gb|AAF80223.1|AC025290_12 Contains similarity
to a cysteine endopeptidase 1 from Phaseolus vulgaris
gb|U52970 and is a member of the papain cysteine
protease family PF|00112. [Arabidopsis thaliana]
Length = 343
Score = 142 bits (359), Expect = 2e-33
Identities = 72/161 (44%), Positives = 106/161 (65%)
Frame = +1
Query: 94 LLMVSNLWIISASECPPMHKKNSSNLEAKRKRFESWQKQHGRKYENPEEWQVRFDIYQTN 273
+L+ S L + +S P HK ++RFE W K H + Y +EW +RF IYQ+N
Sbjct: 19 VLIASKLCSVDSSVYDP-HK-------TLKQRFEKWLKTHSKLYGGRDEWMLRFGIYQSN 70
Query: 274 VEFIECINSQNRSYHLTDNKFADLTNEEFKRIYMGYGKTSLSCNAGAGLLRYNGHGDLPE 453
V+ I+ INS + + LTDN+FAD+TN EFK ++G +SL + + + G++P+
Sbjct: 71 VQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRPV-CDPAGNVPD 129
Query: 454 SIDWRKKGAVTDIKDQGTCGSCWAFSAVAAVEGIHQIKSGN 576
++DWR +GAVT I++QG CG CWAFSAVAA+EGI++IK+GN
Sbjct: 130 AVDWRTQGAVTPIRNQGKCGGCWAFSAVAAIEGINKIKTGN 170
>ref|NP_567983.1| cysteine protease XCP1; protein id: At4g35350.1, supported by cDNA:
gi_6708180 [Arabidopsis thaliana] gi|7435808|pir||T06122
cysteine proteinase (EC 3.4.22.-) F23E12.90 -
Arabidopsis thaliana gi|3080415|emb|CAA18734.1| cysteine
proteinase-like protein [Arabidopsis thaliana]
gi|6708181|gb|AAF25831.1|AF191027_1 papain-type cysteine
endopeptidase XCP1 [Arabidopsis thaliana]
gi|7270487|emb|CAB80252.1| cysteine proteinase-like
protein [Arabidopsis thaliana]
gi|26449881|dbj|BAC42063.1| putative cysteine proteinase
[Arabidopsis thaliana] gi|28827736|gb|AAO50712.1|
unknown protein [Arabidopsis thaliana]
Length = 355
Score = 136 bits (343), Expect = 2e-31
Identities = 71/146 (48%), Positives = 93/146 (63%), Gaps = 1/146 (0%)
Frame = +1
Query: 142 PMHKKNSSNLEAKRKRFESWQKQHGRKYENPEEWQVRFDIYQTNVEFIECINSQNRSYHL 321
P H N+ L + FESW +H + Y++ EE RF++++ N+ I+ N++ SY L
Sbjct: 38 PEHLTNTDKL---LELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWL 94
Query: 322 TDNKFADLTNEEFKRIYMGYGKTSLSCNAGAGL-LRYNGHGDLPESIDWRKKGAVTDIKD 498
N+FADLT+EEFK Y+G K S RY DLP+S+DWRKKGAV +KD
Sbjct: 95 GLNEFADLTHEEFKGRYLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKD 154
Query: 499 QGTCGSCWAFSAVAAVEGIHQIKSGN 576
QG CGSCWAFS VAAVEGI+QI +GN
Sbjct: 155 QGQCGSCWAFSTVAAVEGINQITTGN 180
>gb|AAL60580.1|AF454958_1 senescence-associated cysteine protease [Brassica oleracea]
Length = 485
Score = 132 bits (332), Expect = 3e-30
Identities = 72/167 (43%), Positives = 106/167 (63%), Gaps = 3/167 (1%)
Frame = +1
Query: 85 FFTLLMVSNLWIISASECPPMHKKNSSNLEAKRKR-FESWQKQHGRKYENPEEWQVRFDI 261
F T+++VS+ +S H SS +A+ R +E W +HG+ + E RF+I
Sbjct: 12 FLTMIVVSSAMDMSIISYDKNHHTVSSRSDAEVSRLYEEWLVKHGKAQNSLTEKDRRFEI 71
Query: 262 YQTNVEFIECINSQNRSYHLTDNKFADLTNEEFKRIYMGYGKTSLSCNAGAGLLRYNGH- 438
++ N+ FI+ N +N SY L KFADLTN+E++ +Y+G + L A LRY
Sbjct: 72 FKDNLRFIDEHNGKNLSYRLGLTKFADLTNDEYRSMYLG---SRLKRKATKSSLRYEVRV 128
Query: 439 GD-LPESIDWRKKGAVTDIKDQGTCGSCWAFSAVAAVEGIHQIKSGN 576
GD +PES+DWRK+GAV ++KDQG+CGSCWAFS + AVEGI++I +G+
Sbjct: 129 GDAIPESVDWRKEGAVAEVKDQGSCGSCWAFSTIGAVEGINKIVTGD 175
>gb|AAM73806.1|AF448505_1 cysteine proteinase [Brassica napus]
gi|21666726|gb|AAM73807.1|AF448506_1 cysteine proteinase
[Brassica napus]
Length = 343
Score = 131 bits (329), Expect = 7e-30
Identities = 69/136 (50%), Positives = 88/136 (63%), Gaps = 5/136 (3%)
Frame = +1
Query: 181 RKRFESWQKQHGRKYENPEEWQVRFDIYQTNVEFIECINS--QNRSYHLTDNKFADLTNE 354
+KR +W +HGR Y + E R+ +++ NVE IE +N ++ L N+FADLTNE
Sbjct: 34 QKRHAAWMTEHGRVYADANEKNNRYVVFKRNVESIERLNEVQYGLTFKLAVNQFADLTNE 93
Query: 355 EFKRIYMGY-GKTSLSCNAGAGLLRYN--GHGDLPESIDWRKKGAVTDIKDQGTCGSCWA 525
EF+ +Y GY G + LS RY LP S+DWRKKGAVT IKDQG+CGSCWA
Sbjct: 94 EFRSMYTGYKGNSVLSSRTKPTSFRYQHVSSDALPISVDWRKKGAVTPIKDQGSCGSCWA 153
Query: 526 FSAVAAVEGIHQIKSG 573
FSAVAA+EG+ QIK G
Sbjct: 154 FSAVAAIEGVAQIKKG 169
>gb|AAD53012.1|AF089849_1 senescence-specific cysteine protease [Brassica napus]
Length = 344
Score = 130 bits (328), Expect = 9e-30
Identities = 69/138 (50%), Positives = 87/138 (63%), Gaps = 5/138 (3%)
Frame = +1
Query: 175 AKRKRFESWQKQHGRKYENPEEWQVRFDIYQTNVEFIECINS--QNRSYHLTDNKFADLT 348
A +KR W +HGR Y + E R+ +++ NVE IE +N ++ L N+FADLT
Sbjct: 33 AMQKRHAEWMTEHGRVYADANEKNNRYAVFKRNVERIERLNDVQSGLTFKLAVNQFADLT 92
Query: 349 NEEFKRIYMGY-GKTSLSCNAGAGLLRYNGHGD--LPESIDWRKKGAVTDIKDQGTCGSC 519
NEEF+ +Y G+ G + LS RY LP S+DWRKKGAVT IKDQG CGSC
Sbjct: 93 NEEFRSMYTGFKGNSVLSSRTKPTSFRYQNVSSDALPVSVDWRKKGAVTPIKDQGLCGSC 152
Query: 520 WAFSAVAAVEGIHQIKSG 573
WAFSAVAA+EG+ QIK G
Sbjct: 153 WAFSAVAAIEGVAQIKKG 170
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 494,276,798
Number of Sequences: 1393205
Number of extensions: 10450290
Number of successful extensions: 31852
Number of sequences better than 10.0: 961
Number of HSP's better than 10.0 without gapping: 30219
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 31184
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21426319650
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
