KMC019722A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC019722A_C01 KMC019722A_c01
AAAAGTGGGAAACAGGACCCAACTAAACCGGTATAGTTGGATTTTTTTCTTTTCCTTTCC
TTTAGCTTATCACAATCCCAATTTTAGTAATGATACTTAGACACCTAAACAATATAAACA
TGTCACATACACCTCAATTTTTTTTGGTCTGTTCTCTTACTTTCTCATCATAAATATATT
TCGGGTGTCTGCACTATGTGAATGTTTACAAATCACTTTCATTTTACTAAAATACGTAAT
TATTTTCTCTCTTCTTTATCCCACACCATCTTACTAGTATCCAACCTCACAAAGTTAAGA
AATTCATCATTGAGCCAGTTGTACTTCTCCTTGAAAGGGTTGTTGAGTGTGTTTGGAGGG
TAACTATCAATGCAATCTTTCCACACATTAGCATCAGACAAATCCCTCATCACCTCCCAA
ACACAGTGGTGGCACTTGAACCCAGTTCCAAGGCTTATCATGAGGATC


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC019722A_C01 KMC019722A_c01
         (468 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_196073.1| fatty acid elongase - like protein; protein id:...    87  1e-16
gb|AAG16863.1|AC069145_12 putative fatty acid elongase [Oryza sa...    80  8e-15
ref|NP_180431.1| putative fatty acid elongase; protein id: At2g2...    80  1e-14
gb|AAM61290.1| putative fatty acid elongase [Arabidopsis thaliana]     80  1e-14
ref|NP_172251.1| fatty acid elongase 3-ketoacyl-CoA synthase, pu...    77  7e-14

>ref|NP_196073.1| fatty acid elongase - like protein; protein id: At5g04530.1
           [Arabidopsis thaliana] gi|11357291|pir||T48449 fatty
           acid elongase-like protein - Arabidopsis thaliana
           gi|7406457|emb|CAB85559.1| fatty acid elongase-like
           protein [Arabidopsis thaliana]
           gi|26449447|dbj|BAC41850.1| putative fatty acid elongase
           [Arabidopsis thaliana] gi|28951053|gb|AAO63450.1|
           At5g04530 [Arabidopsis thaliana]
          Length = 464

 Score = 86.7 bits (213), Expect = 1e-16
 Identities = 35/64 (54%), Positives = 52/64 (80%), Gaps = 1/64 (1%)
 Frame = -2

Query: 467 ILMISLGTGFKCHHCVWEVMRDLSDANVWKDCIDSYPP-NTLNNPFKEKYNWLNDEFLNF 291
           ILM+S+G GF+ ++CVWEV++DL D NVW+D +D YP  + + NPF EKY+W+ND+ ++F
Sbjct: 401 ILMMSMGAGFESNNCVWEVLKDLDDKNVWEDSVDRYPELSRIPNPFVEKYDWINDDTMSF 460

Query: 290 VRLD 279
           VR+D
Sbjct: 461 VRVD 464

>gb|AAG16863.1|AC069145_12 putative fatty acid elongase [Oryza sativa]
           gi|16905155|gb|AAL31025.1|AC078948_9 putative fatty acid
           elongase 3-ketoacyl-CoA synthase [Oryza sativa]
          Length = 465

 Score = 80.5 bits (197), Expect = 8e-15
 Identities = 32/54 (59%), Positives = 40/54 (73%)
 Frame = -2

Query: 467 ILMISLGTGFKCHHCVWEVMRDLSDANVWKDCIDSYPPNTLNNPFKEKYNWLND 306
           +LM++ G GFKC+ CVW V + +SDA VWKDCID YPP  L NPF EKY ++ D
Sbjct: 408 VLMLTFGAGFKCNSCVWTVEKPVSDAGVWKDCIDHYPPKELANPFMEKYGFVKD 461

>ref|NP_180431.1| putative fatty acid elongase; protein id: At2g28630.1, supported by
           cDNA: 115769., supported by cDNA: gi_15810262, supported
           by cDNA: gi_20259582 [Arabidopsis thaliana]
           gi|25408004|pir||C84687 probable fatty acid elongase
           [imported] - Arabidopsis thaliana
           gi|4580394|gb|AAD24372.1| putative fatty acid elongase
           [Arabidopsis thaliana] gi|15810263|gb|AAL07019.1|
           putative fatty acid elongase [Arabidopsis thaliana]
           gi|20259583|gb|AAM14134.1| putative fatty acid elongase
           [Arabidopsis thaliana]
          Length = 476

 Score = 80.1 bits (196), Expect = 1e-14
 Identities = 33/59 (55%), Positives = 41/59 (68%), Gaps = 4/59 (6%)
 Frame = -2

Query: 467 ILMISLGTGFKCHHCVWEVMRDL----SDANVWKDCIDSYPPNTLNNPFKEKYNWLNDE 303
           + MIS G GFKC+ CVWEV+RDL    S  NVW  CID YPP ++ NP+ EK+ W+ DE
Sbjct: 406 VFMISFGAGFKCNSCVWEVVRDLTGGESKGNVWNHCIDDYPPKSILNPYLEKFGWIQDE 464

>gb|AAM61290.1| putative fatty acid elongase [Arabidopsis thaliana]
          Length = 476

 Score = 80.1 bits (196), Expect = 1e-14
 Identities = 33/59 (55%), Positives = 41/59 (68%), Gaps = 4/59 (6%)
 Frame = -2

Query: 467 ILMISLGTGFKCHHCVWEVMRDL----SDANVWKDCIDSYPPNTLNNPFKEKYNWLNDE 303
           + MIS G GFKC+ CVWEV+RDL    S  NVW  CID YPP ++ NP+ EK+ W+ DE
Sbjct: 406 VFMISFGAGFKCNSCVWEVVRDLTGGESKGNVWNHCIDDYPPKSILNPYLEKFGWIQDE 464

>ref|NP_172251.1| fatty acid elongase 3-ketoacyl-CoA synthase, putative; protein id:
           At1g07720.1, supported by cDNA: gi_16226846, supported
           by cDNA: gi_17065247 [Arabidopsis thaliana]
           gi|25407000|pir||D86212 hypothetical protein [imported]
           - Arabidopsis thaliana
           gi|8439896|gb|AAF75082.1|AC007583_18 Contains similarity
           to fatty acid elongase 3-ketoacyl-CoA synthase 1 from
           Arabidopsis thaliana gb|AF053345. It contains chalcone
           and stilbene synthases domain PF|00195
           gi|16226847|gb|AAL16279.1|AF428349_1 At1g07720/F24B9_16
           [Arabidopsis thaliana] gi|17065248|gb|AAL32778.1|
           Unknown protein [Arabidopsis thaliana]
           gi|22136232|gb|AAM91194.1| unknown protein [Arabidopsis
           thaliana]
          Length = 478

 Score = 77.4 bits (189), Expect = 7e-14
 Identities = 32/59 (54%), Positives = 42/59 (70%), Gaps = 4/59 (6%)
 Frame = -2

Query: 467 ILMISLGTGFKCHHCVWEVMRDLSDA----NVWKDCIDSYPPNTLNNPFKEKYNWLNDE 303
           + MIS G GFKC+ CVWEV+RDL+      NVW  CI+ YPP ++ NPF EKY W+++E
Sbjct: 407 VFMISFGAGFKCNSCVWEVVRDLNVGEAVGNVWNHCINQYPPKSILNPFFEKYGWIHEE 465

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 414,249,029
Number of Sequences: 1393205
Number of extensions: 8517720
Number of successful extensions: 22468
Number of sequences better than 10.0: 78
Number of HSP's better than 10.0 without gapping: 21763
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22425
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 12232797444
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MFB044a01_f BP037182 1 395
2 MF071e08_f BP032070 6 474




Lotus japonicus
Kazusa DNA Research Institute