>KMC019353A_C01 KMC019353A_c01
tgaatgCTGATGCTCAATCTCTGCTCTAATTGGACTAAGAGGCCTGTGTTGATTCTTCAG
TTTATTCAAGTTAGAAATGTTAATGGTCTTCTATTTATGCGTACCCTTCCTCACACATCT
AAGCTATTCGTCAATCCAGGCTTTGAGGTTGTTTCTTCATTTACTAACAGGCTTTATGCG
GGAGGCATTCGGACTGATGTGCCAGTTCAGTTCCTTCGGCCTCGTGTTCTTGAAGTTAAT
ATGAGACAAGACATGCTGAATTTGCACCCAAAGAAAACTATCTCTCAACTCAGTGAATCT
GATGAGTTTTCACAGGAGGCTATTTACATTGTCCATGGCGGGATTGTTGCTGCGCTGAAA
AATGAAGCATGGACATATCCATCTTGTATATGTCATGGAGAATTGATTGTTCGGACTGGT
GTGTATGAGTGTGTCACCTGCCAGCGGTTTTTCTACACCATGATTCGCAGGTATCGGGTA
AATGTTGAGGTCTTTGATGGTC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC019353A_C01 KMC019353A_c01
         (502 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_175703.1| putative replication protein; protein id: At1g5...    36  0.21
ref|NP_196420.1| glycerophosphodiester phosphodiesterase - like ...    36  0.28
ref|NP_006910.1|ND4_10015 NADH dehydrogenase subunit 4 [Drosophi...    35  0.47
gb|AAF77272.1| NADH dehydrogenase subunit 4 [Drosophila mauritia...    34  0.81
gb|AAF77378.1| NADH dehydrogenase subunit 4 [Drosophila simulans...    34  0.81

>ref|NP_175703.1| putative replication protein; protein id: At1g52950.1 [Arabidopsis
           thaliana] gi|12324642|gb|AAG52278.1|AC019018_15 putative
           replication protein; 94555-97079 [Arabidopsis thaliana]
          Length = 566

 Score = 36.2 bits (82), Expect = 0.21
 Identities = 29/136 (21%), Positives = 60/136 (43%), Gaps = 9/136 (6%)
 Frame = +1

Query: 118 SKLFVNPGFEVVSSFTNRLYAGGIRTDVPVQFLRPRVLEVNMRQDMLNLHPKKTISQLSE 297
           S++F+NP    V  F N L + G+   + +   +PR   + +R++      +KTI++L  
Sbjct: 223 SEVFINPILTEVEEFKNLLPSDGLA--LTIMEPKPRFQPLRVREERSKQFERKTIAELKA 280

Query: 298 SDEFSQEAIYIVHGGIVAALKNEAWTYPSCICHGELI---------VRTGVYECVTCQRF 450
           S  F  E + I+   +   L    + Y    C+ ++           +  +Y C  C   
Sbjct: 281 S--FEVEKVKIICSILSIDLDYSWYYYAHIKCNKKVFKSKKTLSSGAKKIIYRCDKCATD 338

Query: 451 FYTMIRRYRVNVEVFD 498
             ++  RY +++++ D
Sbjct: 339 VTSIEARYWLHLDIMD 354

>ref|NP_196420.1| glycerophosphodiester phosphodiesterase - like protein; protein id:
           At5g08030.1 [Arabidopsis thaliana]
           gi|11357308|pir||T45628 glycerophosphodiester
           phosphodiesterase-like protein - Arabidopsis thaliana
           gi|6562317|emb|CAB62615.1| glycerophosphodiester
           phosphodiesterase-like protein [Arabidopsis thaliana]
          Length = 372

 Score = 35.8 bits (81), Expect = 0.28
 Identities = 20/80 (25%), Positives = 42/80 (52%)
 Frame = +1

Query: 19  LCSNWTKRPVLILQFIQVRNVNGLLFMRTLPHTSKLFVNPGFEVVSSFTNRLYAGGIRTD 198
           L  +W K+P+    FIQ      L+++  +  + KLF+     +++  TN+ YA  I +D
Sbjct: 215 LSEDWLKQPI----FIQSFAATSLVYISNMTDSPKLFLIDDVTILTEDTNKTYA-EITSD 269

Query: 199 VPVQFLRPRVLEVNMRQDML 258
             + +++P V+ +   +D +
Sbjct: 270 AYLDYIKPYVIGIGPWKDTI 289

>ref|NP_006910.1|ND4_10015 NADH dehydrogenase subunit 4 [Drosophila yakuba]
           gi|128746|sp|P07707|NU4M_DROYA NADH-UBIQUINONE
           OXIDOREDUCTASE CHAIN 4 gi|85265|pir||I25797 NADH2
           dehydrogenase (ubiquinone) (EC 1.6.5.3) chain 4 - fruit
           fly (Drosophila yakuba) mitochondrion
           gi|12932|emb|CAA26993.1| NADH dehydrogenase (ubiquinone)
           chain 4 [Drosophila yakuba]
          Length = 446

 Score = 35.0 bits (79), Expect = 0.47
 Identities = 29/105 (27%), Positives = 52/105 (48%), Gaps = 8/105 (7%)
 Frame = +2

Query: 62  LFKLEMLMVFYLCVPFLTHLSYSSIQALRLFLH----LLTGFMRE----AFGLMCQFSSF 217
           + K+ + ++F   V F+ ++ Y  +Q +  F+     L+  FM      ++ L C   S+
Sbjct: 1   MLKIILFLLFLTPVCFINNM-YWMVQIMLFFISFIFLLMNNFMNYWSEISYFLGCDMLSY 59

Query: 218 GLVFLKLI*DKTC*ICTQRKLSLNSVNLMSFHRRLFTLSMAGLLL 352
           GLV L L       IC+   L+  S+N  + ++ LF L++  LLL
Sbjct: 60  GLVLLSLW------ICSLMLLASESINKYNNYKNLFLLNIVILLL 98

>gb|AAF77272.1| NADH dehydrogenase subunit 4 [Drosophila mauritiana]
           gi|8573634|gb|AAF77468.1| NADH dehydrogenase subunit 4
           [Drosophila simulans] gi|8573648|gb|AAF77481.1| NADH
           dehydrogenase subunit 4 [Drosophila simulans]
           gi|8573662|gb|AAF77494.1| NADH dehydrogenase subunit 4
           [Drosophila simulans] gi|8573677|gb|AAF77508.1| NADH
           dehydrogenase subunit 4 [Drosophila simulans]
           gi|8573690|gb|AAF77520.1| NADH dehydrogenase subunit 4
           [Drosophila simulans] gi|8573705|gb|AAF77534.1| NADH
           dehydrogenase subunit 4 [Drosophila simulans]
           gi|8573718|gb|AAF77546.1| NADH dehydrogenase subunit 4
           [Drosophila simulans] gi|8573732|gb|AAF77559.1| NADH
           dehydrogenase subunit 4 [Drosophila simulans]
           gi|8573746|gb|AAF77572.1| NADH dehydrogenase subunit 4
           [Drosophila simulans]
          Length = 446

 Score = 34.3 bits (77), Expect = 0.81
 Identities = 27/109 (24%), Positives = 51/109 (46%)
 Frame = +2

Query: 26  LIGLRGLC*FFSLFKLEMLMVFYLCVPFLTHLSYSSIQALRLFLHLLTGFMREAFGLMCQ 205
           L+ L  LC   +++ +  +M+F++   FL            L  + +  +   ++ L C 
Sbjct: 8   LVFLSPLCFINNMYWMVQIMMFFISFIFL------------LMNNFMNYWSEISYFLGCD 55

Query: 206 FSSFGLVFLKLI*DKTC*ICTQRKLSLNSVNLMSFHRRLFTLSMAGLLL 352
             S+GL+ L L       IC+   L+  S+N  + ++ LF L++  LLL
Sbjct: 56  MLSYGLILLSLW------ICSLMLLASESINKYNNYKNLFLLNIVILLL 98

>gb|AAF77378.1| NADH dehydrogenase subunit 4 [Drosophila simulans]
           gi|8573549|gb|AAF77389.1| NADH dehydrogenase subunit 4
           [Drosophila simulans] gi|8573564|gb|AAF77403.1| NADH
           dehydrogenase subunit 4 [Drosophila simulans]
           gi|8573578|gb|AAF77416.1| NADH dehydrogenase subunit 4
           [Drosophila simulans] gi|8573592|gb|AAF77429.1| NADH
           dehydrogenase subunit 4 [Drosophila simulans]
           gi|8573606|gb|AAF77442.1| NADH dehydrogenase subunit 4
           [Drosophila simulans] gi|8573620|gb|AAF77455.1| NADH
           dehydrogenase subunit 4 [Drosophila simulans]
          Length = 446

 Score = 34.3 bits (77), Expect = 0.81
 Identities = 27/109 (24%), Positives = 51/109 (46%)
 Frame = +2

Query: 26  LIGLRGLC*FFSLFKLEMLMVFYLCVPFLTHLSYSSIQALRLFLHLLTGFMREAFGLMCQ 205
           L+ L  LC   +++ +  +M+F++   FL            L  + +  +   ++ L C 
Sbjct: 8   LVFLSPLCFINNMYWMVQIMMFFISFIFL------------LMNNFMNYWSEISYFLGCD 55

Query: 206 FSSFGLVFLKLI*DKTC*ICTQRKLSLNSVNLMSFHRRLFTLSMAGLLL 352
             S+GL+ L L       IC+   L+  S+N  + ++ LF L++  LLL
Sbjct: 56  MLSYGLILLSLW------ICSLMLLASESINKYNNYKNLFLLNIVILLL 98

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 402,485,767
Number of Sequences: 1393205
Number of extensions: 8176103
Number of successful extensions: 22262
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 21737
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22244
length of database: 448,689,247
effective HSP length: 114
effective length of database: 289,863,877
effective search space used: 15072921604
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFB092f07_f BP040733 1 457 2 MFB020a11_f BP035392 7 502

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