Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC019270A_C01 KMC019270A_c01
(718 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P48631|FD62_SOYBN Omega-6 fatty acid desaturase, endoplasmic ... 414 e-115
gb|AAN87573.1| delta 12 oleic acid desaturase FAD2 [Vernicia for... 402 e-111
gb|AAF80560.1|AF192486_1 omega-6 fatty acid desaturase [Sesamum ... 398 e-110
gb|AAL37484.1|AF331163_1 delta-12 fatty acid desaturase [Gossypi... 391 e-108
dbj|BAC22091.1| delta-12 desaturase [Spinacia oleracea] 391 e-108
>sp|P48631|FD62_SOYBN Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2
gi|7433297|pir||T07688 omega-6 desaturase FAD2-2,
microsomal - soybean gi|904154|gb|AAB00860.1| microsomal
omega-6 desaturase
Length = 383
Score = 414 bits (1065), Expect = e-115
Identities = 191/225 (84%), Positives = 203/225 (89%)
Frame = +1
Query: 43 MGAGGRTSVPQPNRKSETDAVKRVPFEKPPFSLSQVKKAIPPHCFKRSILRSFSYVVYDL 222
MGAGGRT VP NRKSE D +KRVPFEKP FSLSQ+KKAIPPHCF+RS+LRSFSYVVYDL
Sbjct: 1 MGAGGRTDVPPANRKSEVDPLKRVPFEKPQFSLSQIKKAIPPHCFQRSVLRSFSYVVYDL 60
Query: 223 TIAFFLYYVATHYFHLLPRPLAFLAWPTYWAIQGCILTGVWVIAHECGHHAFSDYQWLDD 402
TIAF LYYVATHYFHLLP PL+F YWA+QGCILTGVWVIAHECGHHAFSDYQ LDD
Sbjct: 61 TIAFCLYYVATHYFHLLPGPLSFRGMAIYWAVQGCILTGVWVIAHECGHHAFSDYQLLDD 120
Query: 403 TVGLILHSSLLVPYFSWKYSHRRHHSNTGSLDRDEVFVPKQKSSIGWYSKYLNNPLGRFL 582
VGLILHS+LLVPYFSWKYSHRRHHSNTGSL+RDEVFVPKQKS I WYSKYLNNP GR L
Sbjct: 121 IVGLILHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSCIKWYSKYLNNPPGRVL 180
Query: 583 TITITLTLGWPLYLTFNVSGRPYERFACHFDPYGPIYSDRERLQI 717
T+ +TLTLGWPLYL NVSGRPY+RFACH+DPYGPIYSDRERLQI
Sbjct: 181 TLAVTLTLGWPLYLALNVSGRPYDRFACHYDPYGPIYSDRERLQI 225
>gb|AAN87573.1| delta 12 oleic acid desaturase FAD2 [Vernicia fordii]
Length = 383
Score = 402 bits (1034), Expect = e-111
Identities = 177/225 (78%), Positives = 204/225 (90%)
Frame = +1
Query: 43 MGAGGRTSVPQPNRKSETDAVKRVPFEKPPFSLSQVKKAIPPHCFKRSILRSFSYVVYDL 222
MGAGGR SVP P +K E++ +KRVP KPPF+L Q+KKAIPPHCF+RS+LRSFSYVVYDL
Sbjct: 1 MGAGGRMSVPPPPKKLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDL 60
Query: 223 TIAFFLYYVATHYFHLLPRPLAFLAWPTYWAIQGCILTGVWVIAHECGHHAFSDYQWLDD 402
T+AF YY+AT+YFHLLP+PL+++AWP YWA+QGC+LTGVWVIAHECGHHAFSDYQ LDD
Sbjct: 61 TVAFIFYYIATNYFHLLPQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDD 120
Query: 403 TVGLILHSSLLVPYFSWKYSHRRHHSNTGSLDRDEVFVPKQKSSIGWYSKYLNNPLGRFL 582
VGL+LHS LLVPYFSWK+SHRRHHSNT SL+RDEVFVPK+KSSI W+SKYLNNP GR
Sbjct: 121 IVGLVLHSCLLVPYFSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLF 180
Query: 583 TITITLTLGWPLYLTFNVSGRPYERFACHFDPYGPIYSDRERLQI 717
T+TITLTLGWPLYL FNVSGRPY+RFACH+DPYGPIY+DRER +I
Sbjct: 181 TLTITLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERTEI 225
>gb|AAF80560.1|AF192486_1 omega-6 fatty acid desaturase [Sesamum indicum]
Length = 383
Score = 398 bits (1022), Expect = e-110
Identities = 174/225 (77%), Positives = 196/225 (86%)
Frame = +1
Query: 43 MGAGGRTSVPQPNRKSETDAVKRVPFEKPPFSLSQVKKAIPPHCFKRSILRSFSYVVYDL 222
MGAGGR S P + + + ++RVP+ KPPF+L +KKAIPPHCF+RS+ RSFSYVVYDL
Sbjct: 1 MGAGGRMSDPTTKDEQKKNPLQRVPYAKPPFTLGDIKKAIPPHCFERSVSRSFSYVVYDL 60
Query: 223 TIAFFLYYVATHYFHLLPRPLAFLAWPTYWAIQGCILTGVWVIAHECGHHAFSDYQWLDD 402
I F LYY+AT YFHLLP P +LAWP YWA+QGC+ TG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61 VIVFLLYYIATSYFHLLPSPYCYLAWPIYWAVQGCVCTGIWVIAHECGHHAFSDYQWLDD 120
Query: 403 TVGLILHSSLLVPYFSWKYSHRRHHSNTGSLDRDEVFVPKQKSSIGWYSKYLNNPLGRFL 582
TVGLILHS+LLVPYFSWKYSHRRHHSNTGSL+RDEVFVPK KS + WYSKYLNNPLGR +
Sbjct: 121 TVGLILHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSRVSWYSKYLNNPLGRVI 180
Query: 583 TITITLTLGWPLYLTFNVSGRPYERFACHFDPYGPIYSDRERLQI 717
T+ +TLTLGWPLYL FNVSGRPY RFACHFDPYGPIY+DRERLQI
Sbjct: 181 TLVVTLTLGWPLYLLFNVSGRPYNRFACHFDPYGPIYNDRERLQI 225
>gb|AAL37484.1|AF331163_1 delta-12 fatty acid desaturase [Gossypium hirsutum]
Length = 384
Score = 391 bits (1005), Expect = e-108
Identities = 172/225 (76%), Positives = 200/225 (88%)
Frame = +1
Query: 43 MGAGGRTSVPQPNRKSETDAVKRVPFEKPPFSLSQVKKAIPPHCFKRSILRSFSYVVYDL 222
MGAGGR SVP RK E+ ++KRVP KPPF+LS++KKAIPPHCF+RS++RSFSY+VYD
Sbjct: 1 MGAGGRMSVPPSQRKQESGSMKRVPISKPPFTLSEIKKAIPPHCFQRSLIRSFSYLVYDF 60
Query: 223 TIAFFLYYVATHYFHLLPRPLAFLAWPTYWAIQGCILTGVWVIAHECGHHAFSDYQWLDD 402
+ YYVAT YFH LP+PL+F+AWP YW +QG +LTGVWVIAHECGHHAFSDYQW+DD
Sbjct: 61 ILVSIFYYVATTYFHNLPQPLSFVAWPIYWTLQGSVLTGVWVIAHECGHHAFSDYQWIDD 120
Query: 403 TVGLILHSSLLVPYFSWKYSHRRHHSNTGSLDRDEVFVPKQKSSIGWYSKYLNNPLGRFL 582
TVGLILHSSLLVPYFSWKYSHRRHHSNTGSL+RDEVFVPK++SSI W++KYLNNP GRF+
Sbjct: 121 TVGLILHSSLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKKRSSIRWWAKYLNNPPGRFV 180
Query: 583 TITITLTLGWPLYLTFNVSGRPYERFACHFDPYGPIYSDRERLQI 717
T+TI LTLGWPLYL FNV+GRPYE ACH++PYGPIY+DRERLQI
Sbjct: 181 TVTIQLTLGWPLYLAFNVAGRPYEGLACHYNPYGPIYNDRERLQI 225
>dbj|BAC22091.1| delta-12 desaturase [Spinacia oleracea]
Length = 382
Score = 391 bits (1004), Expect = e-108
Identities = 174/225 (77%), Positives = 200/225 (88%)
Frame = +1
Query: 43 MGAGGRTSVPQPNRKSETDAVKRVPFEKPPFSLSQVKKAIPPHCFKRSILRSFSYVVYDL 222
MGAGGR S+P RK ++DA+ RVP+EKPPF+L Q+KKAIPPHCFKRS+LRSFSYVVYD
Sbjct: 1 MGAGGR-SIPPSARKEKSDALNRVPYEKPPFTLGQIKKAIPPHCFKRSVLRSFSYVVYDF 59
Query: 223 TIAFFLYYVATHYFHLLPRPLAFLAWPTYWAIQGCILTGVWVIAHECGHHAFSDYQWLDD 402
TIAF LYYVAT+Y HLLP+P +LAWP Y +QGC+LTGVWVIAHECGHHAFSDYQWLDD
Sbjct: 60 TIAFLLYYVATNYIHLLPKPFNYLAWPVYGFVQGCVLTGVWVIAHECGHHAFSDYQWLDD 119
Query: 403 TVGLILHSSLLVPYFSWKYSHRRHHSNTGSLDRDEVFVPKQKSSIGWYSKYLNNPLGRFL 582
TVGL+LHS LLVPYFSWKYSHRRHHSNTGS+++DEVFVP++K ++ W+SKYL+NP GR L
Sbjct: 120 TVGLVLHSFLLVPYFSWKYSHRRHHSNTGSMEKDEVFVPQRKENMSWFSKYLSNPPGRIL 179
Query: 583 TITITLTLGWPLYLTFNVSGRPYERFACHFDPYGPIYSDRERLQI 717
T+ +TLTLGWPLYL FNVSGR YERFACH+DP PIYSDRERLQI
Sbjct: 180 TLVVTLTLGWPLYLLFNVSGRKYERFACHYDPSSPIYSDRERLQI 224
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 726,006,543
Number of Sequences: 1393205
Number of extensions: 17959621
Number of successful extensions: 56815
Number of sequences better than 10.0: 273
Number of HSP's better than 10.0 without gapping: 52079
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 56531
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 33217548346
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
