Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC019131A_C01 KMC019131A_c01
(579 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAO25511.1| 4-coumarate:CoA ligase-like [Nicotiana sylvestris... 184 5e-46
ref|NP_176482.1| 4-coumarate:coenzyme A ligase, putative; protei... 173 1e-42
emb|CAD37115.1| OSJNBa0033H08.4 [Oryza sativa (japonica cultivar... 163 2e-39
gb|AAL02145.1| 4-coumarate:CoA ligase [Populus tomentosa] 142 3e-33
gb|AAL02144.1| 4-coumarate:CoA ligase [Populus tomentosa] 142 3e-33
>gb|AAO25511.1| 4-coumarate:CoA ligase-like [Nicotiana sylvestris]
gi|28628067|gb|AAO25512.1| 4-coumarate:CoA ligase-like
[Nicotiana sylvestris]
Length = 551
Score = 184 bits (468), Expect = 5e-46
Identities = 90/118 (76%), Positives = 107/118 (90%)
Frame = -1
Query: 579 IDDEENVFIVDRIKELIKYKGFQVAPAELEAILLSHPSIEDAAVVSLPDEEAGEIPAASV 400
IDD+ ++F+VDRIKELIKYKGFQVAPAELE ILL+HPS+EDAAVV LPDEEAGEIP A V
Sbjct: 432 IDDDGDIFLVDRIKELIKYKGFQVAPAELEGILLTHPSVEDAAVVGLPDEEAGEIPVAWV 491
Query: 399 VLSLGAKESEEDIMNYVASNAASYKKVRVVHFVESIPKSPSGKIMRRLVKEKMVEKMK 226
VL+ AKESEEDI+NY+AS A YK+VRVV FV+SIPKSPSGKI+RRL+K+KM+E++K
Sbjct: 492 VLNSKAKESEEDIINYIASTVAQYKRVRVVQFVDSIPKSPSGKILRRLIKDKMLERLK 549
>ref|NP_176482.1| 4-coumarate:coenzyme A ligase, putative; protein id: At1g62940.1
[Arabidopsis thaliana] gi|25293416|pir||B96654
hypothetical protein F16P17.9 [imported] - Arabidopsis
thaliana gi|8493582|gb|AAF75805.1|AC011000_8 Strong
similarity to 4-coumarate:CoA ligase 2 gene from
Arabidopsis thaliana gb|AF106085, and contains
AMP-binding PF|00501 and Thioredoxin PF|00085 domains.
EST gb|AA728438 comes from this gene
Length = 542
Score = 173 bits (439), Expect = 1e-42
Identities = 86/114 (75%), Positives = 102/114 (89%)
Frame = -1
Query: 579 IDDEENVFIVDRIKELIKYKGFQVAPAELEAILLSHPSIEDAAVVSLPDEEAGEIPAASV 400
IDD+ ++FIVDRIKELIKYKGFQVAPAELEAILL+HPS+ED AVV LPDEEAGEIPAA V
Sbjct: 424 IDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACV 483
Query: 399 VLSLGAKESEEDIMNYVASNAASYKKVRVVHFVESIPKSPSGKIMRRLVKEKMV 238
V++ A E EEDI+N+VA+N A YKKVR VHFV+SIPKS SGKIMRRL+++K++
Sbjct: 484 VINPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537
>emb|CAD37115.1| OSJNBa0033H08.4 [Oryza sativa (japonica cultivar-group)]
Length = 555
Score = 163 bits (412), Expect = 2e-39
Identities = 79/121 (65%), Positives = 102/121 (84%)
Frame = -1
Query: 579 IDDEENVFIVDRIKELIKYKGFQVAPAELEAILLSHPSIEDAAVVSLPDEEAGEIPAASV 400
ID + +VFIVDRIKELIKYKGFQVAPAELEA+LLSHPS+EDAAV +PDEEAGE+P A V
Sbjct: 435 IDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVACV 494
Query: 399 VLSLGAKESEEDIMNYVASNAASYKKVRVVHFVESIPKSPSGKIMRRLVKEKMVEKMKTN 220
V GA+E EE+I+ YVA ASYK+VRV+H V++IPKS SGKI+RR ++++ +++MK +
Sbjct: 495 VRRHGAEEGEEEIVAYVAERVASYKRVRVLHIVDAIPKSVSGKILRRQLRDEFIKRMKPS 554
Query: 219 S 217
+
Sbjct: 555 A 555
>gb|AAL02145.1| 4-coumarate:CoA ligase [Populus tomentosa]
Length = 536
Score = 142 bits (358), Expect = 3e-33
Identities = 66/113 (58%), Positives = 92/113 (81%)
Frame = -1
Query: 579 IDDEENVFIVDRIKELIKYKGFQVAPAELEAILLSHPSIEDAAVVSLPDEEAGEIPAASV 400
IDD++ +FIVDR+KELIKYKGFQVAPAELEA+L++HP I DAAVV L DE+AGE+P A V
Sbjct: 421 IDDDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPEISDAAVVGLKDEDAGEVPVAFV 480
Query: 399 VLSLGAKESEEDIMNYVASNAASYKKVRVVHFVESIPKSPSGKIMRRLVKEKM 241
V S ++ +E++I Y++ YK+++ V F+E+IPK+PSGKI+R+ +KEK+
Sbjct: 481 VKSEKSQATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKNLKEKL 533
>gb|AAL02144.1| 4-coumarate:CoA ligase [Populus tomentosa]
Length = 536
Score = 142 bits (358), Expect = 3e-33
Identities = 66/113 (58%), Positives = 92/113 (81%)
Frame = -1
Query: 579 IDDEENVFIVDRIKELIKYKGFQVAPAELEAILLSHPSIEDAAVVSLPDEEAGEIPAASV 400
IDD++ +FIVDR+KELIKYKGFQVAPAELEA+L++HP I DAAVV L DE+AGE+P A V
Sbjct: 421 IDDDDELFIVDRLKELIKYKGFQVAPAELEALLIAHPEISDAAVVGLKDEDAGEVPVAFV 480
Query: 399 VLSLGAKESEEDIMNYVASNAASYKKVRVVHFVESIPKSPSGKIMRRLVKEKM 241
V S ++ +E++I Y++ YK+++ V F+E+IPK+PSGKI+R+ +KEK+
Sbjct: 481 VKSEKSQATEDEIKQYISKQVIFYKRIKRVFFIEAIPKAPSGKILRKNLKEKL 533
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 465,434,919
Number of Sequences: 1393205
Number of extensions: 9943067
Number of successful extensions: 33246
Number of sequences better than 10.0: 1553
Number of HSP's better than 10.0 without gapping: 28232
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32140
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21426319650
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
