Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC019109A_C01 KMC019109A_c01
(556 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P46488|MDHG_CUCSA Malate dehydrogenase, glyoxysomal precursor... 307 5e-83
ref|NP_196528.1| microbody NAD-dependent malate dehydrogenase; p... 305 2e-82
gb|AAL15313.1| AT5g09660/F17I14_150 [Arabidopsis thaliana] 305 2e-82
sp|P19446|MDHG_CITLA Malate dehydrogenase, glyoxysomal precursor... 305 2e-82
sp|Q9XFW3|MDHH_BRANA Malate dehydrogenase 2, glyoxysomal precurs... 298 2e-80
>sp|P46488|MDHG_CUCSA Malate dehydrogenase, glyoxysomal precursor gi|1076276|pir||S52039
malate dehydrogenase (EC 1.1.1.37) - cucumber
gi|695311|gb|AAC41647.1| glyoxysomal malate
dehydrogenase
Length = 356
Score = 307 bits (787), Expect = 5e-83
Identities = 152/182 (83%), Positives = 163/182 (89%)
Frame = +1
Query: 10 MEAHAGATQRIARISAHLNPSNFQEGGDVLLKRGNCRGKGGAPGFKVAILGAAGGIGQSL 189
M+ QRIARISAHL+P +Q +L+R NCR KGGAPGFKVAILGAAGGIGQ L
Sbjct: 1 MQPIPDVNQRIARISAHLHPPKYQMEESSVLRRANCRAKGGAPGFKVAILGAAGGIGQPL 60
Query: 190 SLLMKMNPLVSVLHLYDVVNSPGVTADVSHMDTGAVVRGFLGQPQLESALTGMDLVIIPA 369
++LMKMNPLVSVLHLYDVVN+PGVTAD+SHMDTGAVVRGFLGQ QLE ALTGMDLV+IPA
Sbjct: 61 AMLMKMNPLVSVLHLYDVVNAPGVTADISHMDTGAVVRGFLGQQQLERALTGMDLVVIPA 120
Query: 370 GVPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVNSTVPIAAEVFKKAGT 549
GVPRKPGMTRDDLFKINAGIVKTLCEG+AKCCP AIVNLISNPVNSTVPIAAEVFKKAGT
Sbjct: 121 GVPRKPGMTRDDLFKINAGIVKTLCEGIAKCCPTAIVNLISNPVNSTVPIAAEVFKKAGT 180
Query: 550 YD 555
YD
Sbjct: 181 YD 182
>ref|NP_196528.1| microbody NAD-dependent malate dehydrogenase; protein id:
At5g09660.1, supported by cDNA: gi_14335145, supported
by cDNA: gi_16226936, supported by cDNA: gi_16323156,
supported by cDNA: gi_18655350 [Arabidopsis thaliana]
gi|11133713|sp|Q9ZP05|MDHG_ARATH Malate dehydrogenase,
glyoxysomal precursor (mbNAD-MDH)
gi|11251113|pir||T49932 malate dehydrogenase (EC
1.1.1.37) precursor, NAD-dependent, glyoxysomal
[validated] - Arabidopsis thaliana
gi|3929651|emb|CAA10321.1| microbody NAD-dependent
malate dehydrogenase [Arabidopsis thaliana]
gi|7671423|emb|CAB89364.1| microbody NAD-dependent
malate dehydrogenase [Arabidopsis thaliana]
gi|9758994|dbj|BAB09521.1| microbody NAD-dependent
malate dehydrogenase [Arabidopsis thaliana]
gi|14335146|gb|AAK59853.1| AT5g09660/F17I14_150
[Arabidopsis thaliana]
gi|16226937|gb|AAL16303.1|AF428373_1
AT5g09660/F17I14_150 [Arabidopsis thaliana]
gi|18655351|gb|AAL76131.1| AT5g09660/F17I14_150
[Arabidopsis thaliana]
Length = 354
Score = 305 bits (782), Expect = 2e-82
Identities = 156/182 (85%), Positives = 162/182 (88%)
Frame = +1
Query: 10 MEAHAGATQRIARISAHLNPSNFQEGGDVLLKRGNCRGKGGAPGFKVAILGAAGGIGQSL 189
ME A QRIARISAHL P E + ++ R NCR KGG PGFKVAILGAAGGIGQSL
Sbjct: 1 MEFRGDANQRIARISAHLTPQ--MEAKNSVIGRENCRAKGGNPGFKVAILGAAGGIGQSL 58
Query: 190 SLLMKMNPLVSVLHLYDVVNSPGVTADVSHMDTGAVVRGFLGQPQLESALTGMDLVIIPA 369
SLLMKMNPLVS+LHLYDVVN+PGVTADVSHMDTGAVVRGFLG QLE ALTGMDLVIIPA
Sbjct: 59 SLLMKMNPLVSLLHLYDVVNAPGVTADVSHMDTGAVVRGFLGAKQLEDALTGMDLVIIPA 118
Query: 370 GVPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVNSTVPIAAEVFKKAGT 549
G+PRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVNSTVPIAAEVFKKAGT
Sbjct: 119 GIPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVNSTVPIAAEVFKKAGT 178
Query: 550 YD 555
YD
Sbjct: 179 YD 180
>gb|AAL15313.1| AT5g09660/F17I14_150 [Arabidopsis thaliana]
Length = 354
Score = 305 bits (781), Expect = 2e-82
Identities = 155/182 (85%), Positives = 162/182 (88%)
Frame = +1
Query: 10 MEAHAGATQRIARISAHLNPSNFQEGGDVLLKRGNCRGKGGAPGFKVAILGAAGGIGQSL 189
ME A QRIARISAHL P E + ++ R NCR KGG PGFKVAILGAAGGIGQSL
Sbjct: 1 MEFRGDANQRIARISAHLTPQ--MEAKNSVIGRENCRAKGGNPGFKVAILGAAGGIGQSL 58
Query: 190 SLLMKMNPLVSVLHLYDVVNSPGVTADVSHMDTGAVVRGFLGQPQLESALTGMDLVIIPA 369
SLLMKMNPLVS+LHLYDVVN+PGVTADVSHMDTGAVVRGFLG QLE ALTGMDLVIIPA
Sbjct: 59 SLLMKMNPLVSLLHLYDVVNAPGVTADVSHMDTGAVVRGFLGAKQLEDALTGMDLVIIPA 118
Query: 370 GVPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVNSTVPIAAEVFKKAGT 549
G+PRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVNST+PIAAEVFKKAGT
Sbjct: 119 GIPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVNSTIPIAAEVFKKAGT 178
Query: 550 YD 555
YD
Sbjct: 179 YD 180
>sp|P19446|MDHG_CITLA Malate dehydrogenase, glyoxysomal precursor gi|319832|pir||DEPUGW
malate dehydrogenase (EC 1.1.1.37) precursor,
glyoxysomal - watermelon gi|167284|gb|AAA33041.1|
glyoxysomal malate dehydrogenase precursor (EC 1.1.1.37)
Length = 356
Score = 305 bits (781), Expect = 2e-82
Identities = 151/182 (82%), Positives = 162/182 (88%)
Frame = +1
Query: 10 MEAHAGATQRIARISAHLNPSNFQEGGDVLLKRGNCRGKGGAPGFKVAILGAAGGIGQSL 189
M+ QRIARISAHL+P Q L+R NCR KGGAPGFKVAILGAAGGIGQ L
Sbjct: 1 MQPIPDVNQRIARISAHLHPPKSQMEESSALRRANCRAKGGAPGFKVAILGAAGGIGQPL 60
Query: 190 SLLMKMNPLVSVLHLYDVVNSPGVTADVSHMDTGAVVRGFLGQPQLESALTGMDLVIIPA 369
++LMKMNPLVSVLHLYDVVN+PGVTAD+SHMDTGAVVRGFLGQ QLE+ALTGMDL+I+PA
Sbjct: 61 AMLMKMNPLVSVLHLYDVVNAPGVTADISHMDTGAVVRGFLGQQQLEAALTGMDLIIVPA 120
Query: 370 GVPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVNSTVPIAAEVFKKAGT 549
GVPRKPGMTRDDLFKINAGIVKTLCEG+AKCCP AIVNLISNPVNSTVPIAAEVFKKAGT
Sbjct: 121 GVPRKPGMTRDDLFKINAGIVKTLCEGIAKCCPRAIVNLISNPVNSTVPIAAEVFKKAGT 180
Query: 550 YD 555
YD
Sbjct: 181 YD 182
>sp|Q9XFW3|MDHH_BRANA Malate dehydrogenase 2, glyoxysomal precursor
gi|4995091|emb|CAB43995.1| malate dehydrogenase 2
[Brassica napus]
Length = 358
Score = 298 bits (764), Expect = 2e-80
Identities = 155/184 (84%), Positives = 161/184 (87%), Gaps = 2/184 (1%)
Frame = +1
Query: 10 MEAHAGATQRIARISAHLNPSNF--QEGGDVLLKRGNCRGKGGAPGFKVAILGAAGGIGQ 183
ME A +RIA ISAHL PS E + ++ R NCR KGG PGFKVAILGAAGGIGQ
Sbjct: 1 MEFRGDANKRIAMISAHLQPSFTPQMEAKNSVMGRENCRAKGGNPGFKVAILGAAGGIGQ 60
Query: 184 SLSLLMKMNPLVSVLHLYDVVNSPGVTADVSHMDTGAVVRGFLGQPQLESALTGMDLVII 363
SLSLLMKMNPLVS+LHLYDVVN+PGVTADVSHMDTGAVVRGFLG QLE ALTGMDLVII
Sbjct: 61 SLSLLMKMNPLVSLLHLYDVVNAPGVTADVSHMDTGAVVRGFLGAKQLEDALTGMDLVII 120
Query: 364 PAGVPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVNSTVPIAAEVFKKA 543
PAGVPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVNSTV IAAEVFKKA
Sbjct: 121 PAGVPRKPGMTRDDLFKINAGIVKTLCEGVAKCCPNAIVNLISNPVNSTVAIAAEVFKKA 180
Query: 544 GTYD 555
GTYD
Sbjct: 181 GTYD 184
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 529,670,163
Number of Sequences: 1393205
Number of extensions: 12567220
Number of successful extensions: 45672
Number of sequences better than 10.0: 628
Number of HSP's better than 10.0 without gapping: 42211
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 45278
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19521267756
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
