KMC018660A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC018660A_C01 KMC018660A_c01
gagaaaaggtaaaatttaagttgatacttgtacagaaagtagaCAACATGAATACCAGTA
TCATTACAnTGCGAATGACAAAAAATGAAGAATGACAGTAATAAAGTACAAGTTAGTGAA
TTTATGGCAACTTTACAGTGATGTAATGACATAATAAATTAAGCTTAAAGTTATTTAATC
AATGAAACTGGCACTTCAAACATCTCCTCACAGAAATTTTTCTGTAATCTGCTCGACAAG
ACAAACTTCACCAACATCAACAATCACCTACATTGCTGCACCTGTATCTAGTATCTCTCC
AAACATACTTGATGAAATCTAACATTCCTTTACTCGATGCATTTGTAGGGCATTATGGTG
CAAAAGTGGCGTATCCATAGATGCATGATTAGGCGGAGTAGATTGAAGGAAATACAAGTT
GATATTTCTTGCTATCCTATTATCATTACACAACAGGTATGCTGATGGATCCCATGTCAA
GTACTCTTGGCAGGATCTCCCCCAATCGCGCATTGATTTTCAAGGGCACAAAATCATCTG
CACGTGTCACACCCACATTTACAATCGCAGTAGCAGCACCAGCCTCA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC018660A_C01 KMC018660A_c01
         (587 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

emb|CAC05449.1| SIR2-family protein [Arabidopsis thaliana]             73  2e-12
ref|NP_568207.1| SIR2-family protein; protein id: At5g09230.1, s...    73  2e-12
gb|AAB95634.1| unknown function; 60% similar to Z50177 (PID:g927...    39  0.049
ref|NP_036372.1| sirtuin 4; sirtuin (silent mating type informat...    39  0.049
ref|NP_572241.2| CG3187-PC [Drosophila melanogaster] gi|28381562...    37  0.14

>emb|CAC05449.1| SIR2-family protein [Arabidopsis thaliana]
          Length = 451

 Score = 73.2 bits (178), Expect = 2e-12
 Identities = 35/46 (76%), Positives = 41/46 (89%)
 Frame = -2

Query: 586 EAGAATAIVNVGVTRADDFVPLKINARLGEILPRVLDMGSISIPVV 449
           EAGA TAIVN+G TRADD VPLKINAR+GEIL RVLD+GS+S+P +
Sbjct: 406 EAGAMTAIVNIGETRADDIVPLKINARVGEILHRVLDVGSLSVPAL 451

>ref|NP_568207.1| SIR2-family protein; protein id: At5g09230.1, supported by cDNA:
           gi_15028032 [Arabidopsis thaliana]
           gi|15028033|gb|AAK76547.1| putative SIR2-family protein
           [Arabidopsis thaliana] gi|21689769|gb|AAM67528.1|
           putative SIR2-family protein [Arabidopsis thaliana]
          Length = 373

 Score = 73.2 bits (178), Expect = 2e-12
 Identities = 35/46 (76%), Positives = 41/46 (89%)
 Frame = -2

Query: 586 EAGAATAIVNVGVTRADDFVPLKINARLGEILPRVLDMGSISIPVV 449
           EAGA TAIVN+G TRADD VPLKINAR+GEIL RVLD+GS+S+P +
Sbjct: 328 EAGAMTAIVNIGETRADDIVPLKINARVGEILHRVLDVGSLSVPAL 373

>gb|AAB95634.1| unknown function; 60% similar to Z50177 (PID:g927403) (PID:g927402)
           [Homo sapiens]
          Length = 326

 Score = 38.9 bits (89), Expect = 0.049
 Identities = 16/29 (55%), Positives = 23/29 (79%)
 Frame = -2

Query: 568 AIVNVGVTRADDFVPLKINARLGEILPRV 482
           AI+N+G TR+DD   LK+N+R GE+LP +
Sbjct: 295 AILNIGPTRSDDLACLKLNSRCGELLPLI 323

>ref|NP_036372.1| sirtuin 4; sirtuin (silent mating type information regulation 2, S.
           cerevisiae, homolog) 4; sirtuin type 4; silent mating
           type information regulation 2, S. cerevisiae, homolog 4;
           sir2-like 4 [Homo sapiens]
           gi|5225324|gb|AAD40852.1|AF083109_1 sirtuin type 4 [Homo
           sapiens]
          Length = 314

 Score = 38.9 bits (89), Expect = 0.049
 Identities = 16/29 (55%), Positives = 23/29 (79%)
 Frame = -2

Query: 568 AIVNVGVTRADDFVPLKINARLGEILPRV 482
           AI+N+G TR+DD   LK+N+R GE+LP +
Sbjct: 283 AILNIGPTRSDDLACLKLNSRCGELLPLI 311

>ref|NP_572241.2| CG3187-PC [Drosophila melanogaster] gi|28381562|gb|AAN09146.2|
           CG3187-PC [Drosophila melanogaster]
          Length = 312

 Score = 37.4 bits (85), Expect = 0.14
 Identities = 14/30 (46%), Positives = 23/30 (76%)
 Frame = -2

Query: 565 IVNVGVTRADDFVPLKINARLGEILPRVLD 476
           IVN+G TRAD    +KI+A+ G+++P++ D
Sbjct: 275 IVNIGETRADHLADIKISAKCGDVIPKLFD 304

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 470,970,312
Number of Sequences: 1393205
Number of extensions: 9800774
Number of successful extensions: 23807
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 22620
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23713
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 22283372436
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 SPD063f12_f BP049046 1 546
2 MFB021b04_f BP035474 44 587




Lotus japonicus
Kazusa DNA Research Institute