Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC018532A_C01 KMC018532A_c01
(570 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_177683.1| transcription initiation factor, putative; prot... 192 2e-48
dbj|BAB89849.1| putative transcription initiation factor [Oryza ... 167 9e-41
ref|NP_190795.1| transcription initiation factor IIF, beta subun... 135 3e-31
pir||T46097 hypothetical protein T25B15.40 - Arabidopsis thalian... 127 1e-28
sp|Q03123|T2FB_XENLA TRANSCRIPTION INITIATION FACTOR IIF, BETA S... 71 8e-12
>ref|NP_177683.1| transcription initiation factor, putative; protein id: At1g75510.1,
supported by cDNA: gi_12642907, supported by cDNA:
gi_13265438 [Arabidopsis thaliana]
gi|9369371|gb|AAF87120.1|AC006434_16 F10A5.27
[Arabidopsis thaliana]
gi|11692842|gb|AAG40024.1|AF324673_1 At1g75700
[Arabidopsis thaliana]
gi|11935195|gb|AAG42013.1|AF327423_1 putative
transcription initiation factor [Arabidopsis thaliana]
gi|12642908|gb|AAK00396.1|AF339714_1 putative
transcription initiation factor [Arabidopsis thaliana]
Length = 261
Score = 192 bits (489), Expect = 2e-48
Identities = 92/107 (85%), Positives = 99/107 (91%)
Frame = -2
Query: 569 NDRGVLMRPMPGMIGLVSSNSKDKKRTQPVKQSDTKRTRRDRGELEDIMFKLFERQPNWA 390
NDRGV MRPMPGM+GLVSSNSK+K++ PVKQ++ KRTRRDRGELE IMFKLFE QPNW
Sbjct: 150 NDRGVHMRPMPGMLGLVSSNSKEKRKPPPVKQTEVKRTRRDRGELEAIMFKLFEGQPNWT 209
Query: 389 LKQLVQETDQPAQFLKEILNELCVYNKRGANQGTYELKPEYKKSVED 249
LKQLVQETDQPAQFLKEILNELCVYNKRG+NQGTYELKPEYKKS ED
Sbjct: 210 LKQLVQETDQPAQFLKEILNELCVYNKRGSNQGTYELKPEYKKSAED 256
>dbj|BAB89849.1| putative transcription initiation factor [Oryza sativa (japonica
cultivar-group)]
Length = 261
Score = 167 bits (423), Expect = 9e-41
Identities = 80/108 (74%), Positives = 92/108 (85%), Gaps = 1/108 (0%)
Frame = -2
Query: 569 NDRGVLMRPMPGMIGLVSSNSKDK-KRTQPVKQSDTKRTRRDRGELEDIMFKLFERQPNW 393
ND G+ MRP+PG++GL+SS K K K+ PVK SD KRTRRDR ELE+I+FKLFERQPNW
Sbjct: 148 NDNGMSMRPLPGLVGLMSSGPKQKEKKPLPVKPSDMKRTRRDRRELENILFKLFERQPNW 207
Query: 392 ALKQLVQETDQPAQFLKEILNELCVYNKRGANQGTYELKPEYKKSVED 249
+LK L+QETDQP QFLKEILN+LC YNKRG NQGT+ELKPEYKKS ED
Sbjct: 208 SLKNLMQETDQPEQFLKEILNDLCFYNKRGPNQGTHELKPEYKKSTED 255
>ref|NP_190795.1| transcription initiation factor IIF, beta subunit, putative;
protein id: At3g52270.1 [Arabidopsis thaliana]
Length = 364
Score = 135 bits (341), Expect = 3e-31
Identities = 64/107 (59%), Positives = 85/107 (78%)
Frame = -2
Query: 569 NDRGVLMRPMPGMIGLVSSNSKDKKRTQPVKQSDTKRTRRDRGELEDIMFKLFERQPNWA 390
N RG+ MRPMPGMI + ++ + KK T + S+ KRTRRDR E+E++MF LFERQ NW
Sbjct: 261 NARGMHMRPMPGMI-IPTAAPEKKKLTN--RTSEMKRTRRDRREMEEVMFNLFERQSNWT 317
Query: 389 LKQLVQETDQPAQFLKEILNELCVYNKRGANQGTYELKPEYKKSVED 249
L+ L+QETDQP QFLK++L +LC+YN +G+NQGTYELKPEYKK+ ++
Sbjct: 318 LRLLIQETDQPEQFLKDLLKDLCIYNNKGSNQGTYELKPEYKKATQE 364
>pir||T46097 hypothetical protein T25B15.40 - Arabidopsis thaliana
gi|10045560|emb|CAC07918.1| putative protein
[Arabidopsis thaliana]
Length = 376
Score = 127 bits (318), Expect = 1e-28
Identities = 64/119 (53%), Positives = 85/119 (70%), Gaps = 12/119 (10%)
Frame = -2
Query: 569 NDRGVLMRPMPGMIGLVSSNSKDKKRTQPVKQSDTKRTRRDRGELEDIMFKLFERQPNWA 390
N RG+ MRPMPGMI + ++ + KK T + S+ KRTRRDR E+E++MF LFERQ NW
Sbjct: 261 NARGMHMRPMPGMI-IPTAAPEKKKLTN--RTSEMKRTRRDRREMEEVMFNLFERQSNWT 317
Query: 389 LKQLVQETDQP------------AQFLKEILNELCVYNKRGANQGTYELKPEYKKSVED 249
L+ L+QETDQP QFLK++L +LC+YN +G+NQGTYELKPEYKK+ ++
Sbjct: 318 LRLLIQETDQPEVCVAYGKVYLVQQFLKDLLKDLCIYNNKGSNQGTYELKPEYKKATQE 376
>sp|Q03123|T2FB_XENLA TRANSCRIPTION INITIATION FACTOR IIF, BETA SUBUNIT (TFIIF-BETA)
(TRANSCRIPTION INITIATION FACTOR RAP30)
gi|422624|pir||S30220 transcription factor RAP30 -
African clawed frog (fragment) gi|65135|emb|CAA78835.1|
transcription factor RAP30 [Xenopus laevis]
Length = 264
Score = 71.2 bits (173), Expect = 8e-12
Identities = 33/84 (39%), Positives = 53/84 (62%)
Frame = -2
Query: 515 SNSKDKKRTQPVKQSDTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEI 336
SN + + K+ D KR R D+ ++ D++F FE+ + +K LV T QP +LKEI
Sbjct: 172 SNHQYNIEYEKKKKDDGKRARVDKHQVLDMLFSAFEKHQYYNIKDLVDITKQPVTYLKEI 231
Query: 335 LNELCVYNKRGANQGTYELKPEYK 264
L ++ +YN +G ++ T+ELKPEY+
Sbjct: 232 LRDIGIYNMKGTHKNTWELKPEYR 255
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 465,092,277
Number of Sequences: 1393205
Number of extensions: 9503063
Number of successful extensions: 24208
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 23482
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24194
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20956655091
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
