>KMC018483A_C01 KMC018483A_c01
aGCTAGTAAATAAAACCAATTCATTGATTTGGAGCCAAAAGCTCATTACGGTGAGGACAT
AAGTAATTGATGTGGAGGAGATAGCCGTCAACTGAGAAGGTTTATTTTCATCATCACGGT
TTGTTTGGCAAAACAAAATTATGATGGCATGCACTTCAGTTGCCAAAAGTTATGCGAGCA
AGTTTCAGACACTTGATACTCCCTAATTAAAATGAAATTGCAACATCTTTTTTCTTTTAT
GCATGCACTCTAACATTACATTACATATATAAAATTCAGACAACATTTGTTTAATGCAAA
AATTGAGCCAGTGCGTTTTTGACGAGGTAGTTTGCAATGATTCCATTGGTTTTCTCTGTT
GTGTGGAAAGAGTCCCAAAATACATATTTGGAAGCATCCATGCAGCTGAAAAGGCTGGCT
CTGGCTGCATGCGTATCCCATCTCAAACATTCCAGTTGCACAACATGCCATCGATGCCAC
TTGAAACCCGCTATTGGGCAGGCTTTTTAACAACATTCAACAAAATA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC018483A_C01 KMC018483A_c01
         (527 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_178536.1| putative GDSL-motif lipase/hydrolase; protein i...    53  3e-15
gb|AAM65485.1| putative GDSL-motif lipase/hydrolase [Arabidopsis...    53  3e-15
pir||T04802 hypothetical protein F10M23.130 - Arabidopsis thalia...    60  2e-13
ref|NP_567758.1| putative APG protein; protein id: At4g26790.1, ...    60  2e-13
ref|NP_199032.1| GDSL-motif lipase/hydrolase-like protein; prote...    42  7e-08

>ref|NP_178536.1| putative GDSL-motif lipase/hydrolase; protein id: At2g04570.1,
           supported by cDNA: 39762. [Arabidopsis thaliana]
           gi|25336279|pir||A84459 probable GDSL-motif
           lipase/hydrolase [imported] - Arabidopsis thaliana
           gi|4587595|gb|AAD25823.1| putative GDSL-motif
           lipase/hydrolase [Arabidopsis thaliana]
          Length = 350

 Score = 53.1 bits (126), Expect(2) = 3e-15
 Identities = 23/42 (54%), Positives = 30/42 (70%)
 Frame = -1

Query: 422 RASLFSCMDASKYVFWDSFHTTEKTNGIIANYLVKNALAQFL 297
           R + F+C +A KYVFWDSFH T+KTN I+AN L+ +    FL
Sbjct: 309 RNNPFTCTNADKYVFWDSFHPTQKTNHIMANALMNSTFPHFL 350

 Score = 49.7 bits (117), Expect(2) = 3e-15
 Identities = 19/35 (54%), Positives = 26/35 (74%)
 Frame = -3

Query: 513 LLKSLPNSGFQVASMACCATGMFEMGYACSQSQPF 409
           ++K+  + GF+V   ACCATGMFEMGY C ++ PF
Sbjct: 279 IIKNPSSFGFEVVGAACCATGMFEMGYGCQRNNPF 313

>gb|AAM65485.1| putative GDSL-motif lipase/hydrolase [Arabidopsis thaliana]
          Length = 350

 Score = 53.1 bits (126), Expect(2) = 3e-15
 Identities = 23/42 (54%), Positives = 30/42 (70%)
 Frame = -1

Query: 422 RASLFSCMDASKYVFWDSFHTTEKTNGIIANYLVKNALAQFL 297
           R + F+C +A KYVFWDSFH T+KTN I+AN L+ +    FL
Sbjct: 309 RNNPFTCTNADKYVFWDSFHPTQKTNHIMANALMNSTFPHFL 350

 Score = 49.7 bits (117), Expect(2) = 3e-15
 Identities = 19/35 (54%), Positives = 26/35 (74%)
 Frame = -3

Query: 513 LLKSLPNSGFQVASMACCATGMFEMGYACSQSQPF 409
           ++K+  + GF+V   ACCATGMFEMGY C ++ PF
Sbjct: 279 IIKNPSSFGFEVVGAACCATGMFEMGYGCQRNNPF 313

>pir||T04802 hypothetical protein F10M23.130 - Arabidopsis thaliana
           gi|4455202|emb|CAB36525.1| putative APG protein
           [Arabidopsis thaliana] gi|7269531|emb|CAB79534.1|
           putative APG protein [Arabidopsis thaliana]
          Length = 365

 Score = 60.5 bits (145), Expect(2) = 2e-13
 Identities = 25/37 (67%), Positives = 32/37 (85%)
 Frame = -1

Query: 410 FSCMDASKYVFWDSFHTTEKTNGIIANYLVKNALAQF 300
           F+C DASKYVFWDSFH TEKTN I+AN+++K  L++F
Sbjct: 328 FTCSDASKYVFWDSFHPTEKTNAIVANHVLKYDLSRF 364

 Score = 36.2 bits (82), Expect(2) = 2e-13
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = -3

Query: 489 GFQVASMACCATGMFEMGYACSQSQPF 409
           GF+    ACC TG +EM Y C +  PF
Sbjct: 302 GFENVRSACCGTGYYEMSYLCDKMNPF 328

>ref|NP_567758.1| putative APG protein; protein id: At4g26790.1, supported by cDNA:
           2574., supported by cDNA: gi_18252232 [Arabidopsis
           thaliana] gi|18252233|gb|AAL61949.1| putative APG
           protein [Arabidopsis thaliana]
           gi|21387003|gb|AAM47905.1| putative APG protein
           [Arabidopsis thaliana] gi|21554559|gb|AAM63613.1|
           putative APG protein [Arabidopsis thaliana]
          Length = 351

 Score = 60.5 bits (145), Expect(2) = 2e-13
 Identities = 25/37 (67%), Positives = 32/37 (85%)
 Frame = -1

Query: 410 FSCMDASKYVFWDSFHTTEKTNGIIANYLVKNALAQF 300
           F+C DASKYVFWDSFH TEKTN I+AN+++K  L++F
Sbjct: 314 FTCSDASKYVFWDSFHPTEKTNAIVANHVLKYDLSRF 350

 Score = 36.2 bits (82), Expect(2) = 2e-13
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = -3

Query: 489 GFQVASMACCATGMFEMGYACSQSQPF 409
           GF+    ACC TG +EM Y C +  PF
Sbjct: 288 GFENVRSACCGTGYYEMSYLCDKMNPF 314

>ref|NP_199032.1| GDSL-motif lipase/hydrolase-like protein; protein id: At5g42170.1
           [Arabidopsis thaliana] gi|9757962|dbj|BAB08450.1|
           GDSL-motif lipase/hydrolase-like protein [Arabidopsis
           thaliana]
          Length = 319

 Score = 42.0 bits (97), Expect(2) = 7e-08
 Identities = 16/38 (42%), Positives = 26/38 (68%)
 Frame = -1

Query: 410 FSCMDASKYVFWDSFHTTEKTNGIIANYLVKNALAQFL 297
           F+C ++S Y+FWDS+H TEK   II + L+ N + + +
Sbjct: 282 FTCKNSSSYIFWDSYHPTEKAYQIIVDKLLGNYITKLV 319

 Score = 35.8 bits (81), Expect(2) = 7e-08
 Identities = 12/35 (34%), Positives = 24/35 (68%)
 Frame = -3

Query: 513 LLKSLPNSGFQVASMACCATGMFEMGYACSQSQPF 409
           ++++  N GF+V++  CC TG+ E+ + C++  PF
Sbjct: 248 MIENPKNYGFEVSNRGCCGTGLVEVLFLCNKINPF 282

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 456,325,720
Number of Sequences: 1393205
Number of extensions: 9449954
Number of successful extensions: 22152
Number of sequences better than 10.0: 136
Number of HSP's better than 10.0 without gapping: 21540
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22149
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17308240320
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF001b05_f BP028260 1 192 2 MF012c03_f BP028854 2 554 3 SPD051g03_f BP048097 83 501 4 MF002g05_f BP028355 92 576 5 MFB059h08_f BP038313 142 643

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