Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC018277A_C01 KMC018277A_c01
(612 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|O49859|C824_SOYBN Cytochrome P450 82A4 (P450 CP9) gi|7430685|... 153 2e-36
sp|O49858|C823_SOYBN Cytochrome P450 82A3 (P450 CP6) gi|7430684|... 149 2e-35
pir||T07964 (S)-N-methylcoclaurine 3'-hydroxylase (EC 1.1.3.-) -... 149 3e-35
pir||T05942 cytochrome P450 82C1 - soybean gi|2739004|gb|AAB9459... 148 5e-35
pir||T04650 cytochrome P450 F10N7.240 - Arabidopsis thaliana 143 2e-33
>sp|O49859|C824_SOYBN Cytochrome P450 82A4 (P450 CP9) gi|7430685|pir||T07749 probable
cytochrome P450 - soybean gi|2765093|emb|CAA71877.1|
putative cytochrome P450 [Glycine max]
Length = 525
Score = 153 bits (386), Expect = 2e-36
Identities = 67/125 (53%), Positives = 95/125 (75%)
Frame = -2
Query: 611 ESMEDCIVGGYHVPAQTRLLTNISKLQRDPFLYSDPFEFHPERFLTTYKEVDLKGQHFEL 432
E EDC +GGYHV TRL+TNI K+ DP ++SDPFEF P+RFLTT+K++D+KG HF+L
Sbjct: 393 EFAEDCTLGGYHVKKGTRLITNIWKIHTDPNVWSDPFEFKPDRFLTTHKDIDVKGHHFQL 452
Query: 431 IPFGAGRRMCPAMSFALKLMQMTLATLLHGFEIMTVDGGPVDMVEESGLSNIRASPLQVI 252
+PFG+GRR+CP +SF L+ + + LA+ LH FEI+ P+DM E G++N +A+PL+V+
Sbjct: 453 LPFGSGRRVCPGISFGLQTVHLALASFLHSFEILNPSTEPLDMTEAFGVTNTKATPLEVL 512
Query: 251 LTPRL 237
+ P L
Sbjct: 513 VKPCL 517
>sp|O49858|C823_SOYBN Cytochrome P450 82A3 (P450 CP6) gi|7430684|pir||T07748 probable
cytochrome P450 - soybean gi|2765091|emb|CAA71876.1|
putative cytochrome P450 [Glycine max]
Length = 527
Score = 149 bits (377), Expect = 2e-35
Identities = 66/124 (53%), Positives = 92/124 (73%)
Frame = -2
Query: 611 ESMEDCIVGGYHVPAQTRLLTNISKLQRDPFLYSDPFEFHPERFLTTYKEVDLKGQHFEL 432
E E+CI+GGYH+ TRL+ N+ K+ RDP ++SDP EF PERFLTT+K+VDL+G +FEL
Sbjct: 395 EFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDPLEFKPERFLTTHKDVDLRGHNFEL 454
Query: 431 IPFGAGRRMCPAMSFALKLMQMTLATLLHGFEIMTVDGGPVDMVEESGLSNIRASPLQVI 252
+PFG+GRR+C MS L ++ TLA LLH F+I+ PVDM E G +N +A+PL+++
Sbjct: 455 LPFGSGRRVCAGMSLGLNMVHFTLANLLHSFDILNPSAEPVDMTEFFGFTNTKATPLEIL 514
Query: 251 LTPR 240
+ PR
Sbjct: 515 VKPR 518
>pir||T07964 (S)-N-methylcoclaurine 3'-hydroxylase (EC 1.1.3.-) - California
poppy gi|3127031|gb|AAC39454.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 560
Score = 149 bits (376), Expect = 3e-35
Identities = 73/134 (54%), Positives = 95/134 (70%), Gaps = 3/134 (2%)
Frame = -2
Query: 611 ESMEDCIVGGYHVPAQTRLLTNISKLQRDPFLYSDPFEFHPERFLTTYKE---VDLKGQH 441
E++EDC VGGYH+ TRLL NI KLQRDP ++S+P EF PERFL +D +GQH
Sbjct: 426 EAIEDCNVGGYHIKTGTRLLVNIWKLQRDPRVWSNPSEFRPERFLDNQSNGTLLDFRGQH 485
Query: 440 FELIPFGAGRRMCPAMSFALKLMQMTLATLLHGFEIMTVDGGPVDMVEESGLSNIRASPL 261
FE IPFG+GRRMCP ++FA ++ MTLA LL F++ T PVDM E SGL+ + +PL
Sbjct: 486 FEYIPFGSGRRMCPGVNFATLILHMTLARLLQAFDLSTPSSSPVDMTEGSGLTMPKVTPL 545
Query: 260 QVILTPRLFSQVHD 219
+V+LTPRL ++D
Sbjct: 546 KVLLTPRLPLPLYD 559
>pir||T05942 cytochrome P450 82C1 - soybean gi|2739004|gb|AAB94590.1| CYP82C1p
[Glycine max]
Length = 532
Score = 148 bits (374), Expect = 5e-35
Identities = 66/131 (50%), Positives = 100/131 (75%), Gaps = 1/131 (0%)
Frame = -2
Query: 608 SMEDCIV-GGYHVPAQTRLLTNISKLQRDPFLYSDPFEFHPERFLTTYKEVDLKGQHFEL 432
+MEDC GGYH+PA TRL+ N K+ RD ++SDP +F P RFLT++K+VD+KGQ++EL
Sbjct: 401 AMEDCTFSGGYHIPAGTRLMVNAWKIHRDGRVWSDPHDFKPGRFLTSHKDVDVKGQNYEL 460
Query: 431 IPFGAGRRMCPAMSFALKLMQMTLATLLHGFEIMTVDGGPVDMVEESGLSNIRASPLQVI 252
+PFG+GRR CP S AL+++ +T+A LLH F + + VDM E GL+N++A+PL+++
Sbjct: 461 VPFGSGRRACPGASLALRVVHLTMARLLHSFNVASPSNQVVDMTESIGLTNLKATPLEIL 520
Query: 251 LTPRLFSQVHD 219
LTPRL +++++
Sbjct: 521 LTPRLDTKLYE 531
>pir||T04650 cytochrome P450 F10N7.240 - Arabidopsis thaliana
Length = 501
Score = 143 bits (361), Expect = 2e-33
Identities = 67/126 (53%), Positives = 93/126 (73%), Gaps = 1/126 (0%)
Frame = -2
Query: 611 ESMEDCIVGGYHVPAQTRLLTNISKLQRDPFLYSDPFEFHPERFLT-TYKEVDLKGQHFE 435
E+MEDC V GY+VP TRL+ N+ K+QRDP +Y +P EF PERF+T K+ D++GQ+FE
Sbjct: 370 EAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEAKDFDVRGQNFE 429
Query: 434 LIPFGAGRRMCPAMSFALKLMQMTLATLLHGFEIMTVDGGPVDMVEESGLSNIRASPLQV 255
L+PFG+GRR CP S A++++ + LA LH FE+ TV PVDM E GL+ +A+PL+V
Sbjct: 430 LMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVLDRPVDMSESPGLTITKATPLEV 489
Query: 254 ILTPRL 237
++ PRL
Sbjct: 490 LINPRL 495
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 520,667,107
Number of Sequences: 1393205
Number of extensions: 11038449
Number of successful extensions: 25665
Number of sequences better than 10.0: 2548
Number of HSP's better than 10.0 without gapping: 23498
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24019
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 24568846532
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
