KMC018250A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC018250A_C01 KMC018250A_c01
tcattctattcatattttaagatagaatgaagagattatcctattctgatcctatcatac
ttttatacagctctcttattTTACTATTCATAATTCAGAAATGAAATACACACTTTGAAG
GTAGGAATAAAAGATGGACTAGTTGCGAATACACAAACGGGCCTGAAAATATGAAAAAAA
GGAGATCCAAACATTTTCCAGATCAAGACAGATATGACCTTAAGAGATTGTATTTCTGGC
CTACACAATTCATGAATTTCAGTTGACACCAGCTTTCTAGACAATGATCAACTTATAGTT
GGTTGTAGTTGGCCAGAAAAACTCTATTTAATTTCGCTGAAGACCCCTATCTATAACAGC
TTTCAGTCACCGATCACTGAATAATACAATTATTTGGTGAATTTCAAACACCAAACAAGG
ATACAAAACTCAAATCAGCCTCAGTTCATACTCTCCAGCCATGGTTATATATCTCACCCA
TGGAAGAGACTGATACCCACTTTTCTCAATAATCTTCAGATATCTTACCTGTATCCCAGA
CACAGTAAAATATGGTATCTCAAATTTCACACGTATAGGAGCTTTTCTCTCA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC018250A_C01 KMC018250A_c01
         (592 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_176277.1| clathrin adaptor medium chain protein MU1B, put...   107  9e-23
dbj|BAB44014.1| putative clathrin-associated protein [Oryza sati...   107  1e-22
gb|AAF17661.1|AC009398_10 F20B24.16 [Arabidopsis thaliana]             99  5e-20
ref|NP_172543.1| putative clathrin-coat assembly protein; protei...    99  5e-20
ref|NP_493174.1| UNCoordinated locomotion UNC-101, clathrin-asso...    92  4e-18

>ref|NP_176277.1| clathrin adaptor medium chain protein MU1B, putative; protein id:
           At1g60780.1, supported by cDNA: gi_20466371 [Arabidopsis
           thaliana] gi|25320049|pir||C96633 probable
           Serine/Threonine protein kinase F8A5.29 [imported] -
           Arabidopsis thaliana gi|2462748|gb|AAB71967.1| putative
           Clathrin Coat Assembly protein [Arabidopsis thaliana]
           gi|20466372|gb|AAM20503.1| clathrin adaptor medium chain
           protein MU1B, putative [Arabidopsis thaliana]
           gi|25084014|gb|AAN72155.1| clathrin adaptor medium chain
           protein MU1B, putative [Arabidopsis thaliana]
          Length = 428

 Score =  107 bits (268), Expect = 9e-23
 Identities = 51/53 (96%), Positives = 53/53 (99%)
 Frame = -2

Query: 591 ERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQSLPWVRYITMAGEYELRLI 433
           ERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQ+LPWVRYITMAGEYELRL+
Sbjct: 376 ERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRYITMAGEYELRLV 428

>dbj|BAB44014.1| putative clathrin-associated protein [Oryza sativa (japonica
           cultivar-group)] gi|20804602|dbj|BAB92293.1| putative
           clathrin-associated protein [Oryza sativa (japonica
           cultivar-group)]
          Length = 429

 Score =  107 bits (266), Expect = 1e-22
 Identities = 51/53 (96%), Positives = 53/53 (99%)
 Frame = -2

Query: 591 ERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQSLPWVRYITMAGEYELRLI 433
           E+KAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQ+LPWVRYITMAGEYELRLI
Sbjct: 377 EKKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQALPWVRYITMAGEYELRLI 429

>gb|AAF17661.1|AC009398_10 F20B24.16 [Arabidopsis thaliana]
          Length = 411

 Score = 98.6 bits (244), Expect = 5e-20
 Identities = 48/53 (90%), Positives = 50/53 (93%)
 Frame = -2

Query: 591 ERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQSLPWVRYITMAGEYELRLI 433
           ERKAPIRVKFEIP F VSGIQVRYLKIIEKSGYQ+ PWVRYITMAGEYELRL+
Sbjct: 359 ERKAPIRVKFEIPKFIVSGIQVRYLKIIEKSGYQAHPWVRYITMAGEYELRLM 411

>ref|NP_172543.1| putative clathrin-coat assembly protein; protein id: At1g10730.1
           [Arabidopsis thaliana] gi|25320047|pir||G86240
           hypothetical protein [imported] - Arabidopsis thaliana
           gi|4874275|gb|AAD31340.1|AC007354_13 Similar to
           gb|L26291 clathrin-associated protein unc-101 from
           Caenorhabditis elegans and is a member of the PF|00928
           Adapter complexes medium subunit family. [Arabidopsis
           thaliana]
          Length = 428

 Score = 98.6 bits (244), Expect = 5e-20
 Identities = 48/53 (90%), Positives = 50/53 (93%)
 Frame = -2

Query: 591 ERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQSLPWVRYITMAGEYELRLI 433
           ERKAPIRVKFEIP F VSGIQVRYLKIIEKSGYQ+ PWVRYITMAGEYELRL+
Sbjct: 376 ERKAPIRVKFEIPKFIVSGIQVRYLKIIEKSGYQAHPWVRYITMAGEYELRLM 428

>ref|NP_493174.1| UNCoordinated locomotion UNC-101, clathrin-associated protein (48.2
           kD) (unc-101) [Caenorhabditis elegans]
           gi|21542385|sp|P35602|AP47_CAEEL Clathrin coat assembly
           protein AP47 (Clathrin coat associated protein AP47)
           (Golgi adaptor AP-1 47 kDa protein) (HA1 47 kDa subunit)
           (Clathrin assembly protein assembly protein complex 1
           medium chain) (Uncoordinated protein 101)
           gi|14530511|emb|CAB05557.3| C. elegans UNC-101 protein
           (corresponding sequence K11D2.3) [Caenorhabditis
           elegans]
          Length = 422

 Score = 92.4 bits (228), Expect = 4e-18
 Identities = 42/52 (80%), Positives = 47/52 (89%)
 Frame = -2

Query: 591 ERKAPIRVKFEIPYFTVSGIQVRYLKIIEKSGYQSLPWVRYITMAGEYELRL 436
           E + PI+VKFEIPYFT SGIQVRYLKIIEKSGYQ+LPWVRYIT  GEYE+R+
Sbjct: 370 EGRPPIKVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGEYEMRM 421

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 467,792,424
Number of Sequences: 1393205
Number of extensions: 9600365
Number of successful extensions: 18915
Number of sequences better than 10.0: 84
Number of HSP's better than 10.0 without gapping: 18536
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18887
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 22569056698
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 SPD098c03_f BP051811 1 414
2 SPD033a09_f BP046594 102 592




Lotus japonicus
Kazusa DNA Research Institute