Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC018210A_C01 KMC018210A_c01
(711 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM60961.1| seed maturation-like protein [Arabidopsis thaliana] 202 3e-51
ref|NP_197001.1| seed maturation -like protein; protein id: At5g... 202 3e-51
ref|NP_179097.1| unknown protein; protein id: At2g14910.1, suppo... 107 1e-22
gb|AAF21309.1| seed maturation protein PM23 [Glycine max] 94 2e-18
gb|ZP_00056820.1| hypothetical protein [Thermobifida fusca] 69 5e-11
>gb|AAM60961.1| seed maturation-like protein [Arabidopsis thaliana]
Length = 355
Score = 202 bits (515), Expect = 3e-51
Identities = 126/230 (54%), Positives = 154/230 (66%), Gaps = 6/230 (2%)
Frame = +3
Query: 39 MAALSWRPF-ILSRLTDLSPNPLHPPKPPPLFLRRRRCF-----LTSCYADGFSSSSSSS 200
MAA S R F +LSR+TDLS L +PPP R + ++S S S
Sbjct: 1 MAAASARAFFMLSRVTDLSKKKLILHQPPPSSSPHRLPYAPNRAVSSSAVISCLSGGGVS 60
Query: 201 SSDDVVSTRKSTFDRGFTVIANMLRRIEPLDNSVISKGVSDAARDSMKQTISTMLGLLPS 380
S D VSTR+S DRGF VIAN++ RI+PLD SVISKG+SD+A+DSMKQTIS+MLGLLPS
Sbjct: 61 SDDSYVSTRRSKLDRGFAVIANLVNRIQPLDTSVISKGLSDSAKDSMKQTISSMLGLLPS 120
Query: 381 DHFSVTVTVSKHPLHRLLVSSIITGYTLWNAEYRMSLTRNLDIASPCGARDSDCEKRSEI 560
D FSV+VT+S+ PL+RLL+SSIITGYTLWNAEYR+SL RN DI P R + E +S
Sbjct: 121 DQFSVSVTISEQPLYRLLISSIITGYTLWNAEYRVSLRRNFDI--PIDPRKEE-EDQSSK 177
Query: 561 LEVKGGGEDGGEIEVASDLGLKDLENCSSSPRVFGDLPPQALNYIQQLQS 710
V+ G E G ++ DLG E SP+VFGDL P+AL+YIQ LQS
Sbjct: 178 DNVRFGSEKG----MSEDLGNCVEEFERLSPQVFGDLSPEALSYIQLLQS 223
>ref|NP_197001.1| seed maturation -like protein; protein id: At5g14970.1, supported
by cDNA: 106301. [Arabidopsis thaliana]
gi|11346180|pir||T51442 seed maturation-like protein -
Arabidopsis thaliana gi|9755664|emb|CAC01816.1| seed
maturation-like protein [Arabidopsis thaliana]
gi|22655278|gb|AAM98229.1| seed maturation-like protein
[Arabidopsis thaliana]
Length = 355
Score = 202 bits (515), Expect = 3e-51
Identities = 126/230 (54%), Positives = 154/230 (66%), Gaps = 6/230 (2%)
Frame = +3
Query: 39 MAALSWRPF-ILSRLTDLSPNPLHPPKPPPLFLRRRRCF-----LTSCYADGFSSSSSSS 200
MAA S R F +LSR+TDLS L +PPP R + ++S S S
Sbjct: 1 MAAASARAFFMLSRVTDLSKKKLILHQPPPSSSPHRLPYAPNRAVSSSAVISCLSGGGVS 60
Query: 201 SSDDVVSTRKSTFDRGFTVIANMLRRIEPLDNSVISKGVSDAARDSMKQTISTMLGLLPS 380
S D VSTR+S DRGF VIAN++ RI+PLD SVISKG+SD+A+DSMKQTIS+MLGLLPS
Sbjct: 61 SDDSYVSTRRSKLDRGFAVIANLVNRIQPLDTSVISKGLSDSAKDSMKQTISSMLGLLPS 120
Query: 381 DHFSVTVTVSKHPLHRLLVSSIITGYTLWNAEYRMSLTRNLDIASPCGARDSDCEKRSEI 560
D FSV+VT+S+ PL+RLL+SSIITGYTLWNAEYR+SL RN DI P R + E +S
Sbjct: 121 DQFSVSVTISEQPLYRLLISSIITGYTLWNAEYRVSLRRNFDI--PIDPRKEE-EDQSSK 177
Query: 561 LEVKGGGEDGGEIEVASDLGLKDLENCSSSPRVFGDLPPQALNYIQQLQS 710
V+ G E G ++ DLG E SP+VFGDL P+AL+YIQ LQS
Sbjct: 178 DNVRFGSEKG----MSEDLGNCVEEFERLSPQVFGDLSPEALSYIQLLQS 223
>ref|NP_179097.1| unknown protein; protein id: At2g14910.1, supported by cDNA:
gi_17380883, supported by cDNA: gi_19698842, supported
by cDNA: gi_20465934 [Arabidopsis thaliana]
gi|25368604|pir||H84522 hypothetical protein At2g14910
[imported] - Arabidopsis thaliana
gi|3650033|gb|AAC61288.1| unknown protein [Arabidopsis
thaliana] gi|17380884|gb|AAL36254.1| unknown protein
[Arabidopsis thaliana] gi|19698843|gb|AAL91157.1|
unknown protein [Arabidopsis thaliana]
gi|20465935|gb|AAM20153.1| unknown protein [Arabidopsis
thaliana]
Length = 386
Score = 107 bits (268), Expect = 1e-22
Identities = 79/218 (36%), Positives = 113/218 (51%), Gaps = 12/218 (5%)
Frame = +3
Query: 93 PNPLHPPKPP-------PLFLRRRRCFLTSCYADGFSSSSSSSSSDDVVSTRKSTFDRGF 251
P LH P P P F RR R + + S++ SS+ DD S T
Sbjct: 14 PQLLHKPTKPLPFLFLLPRFNRRFRSLTITSSSTTSSNNFSSNCGDDGFSLDDFTLHSDS 73
Query: 252 T-----VIANMLRRIEPLDNSVISKGVSDAARDSMKQTISTMLGLLPSDHFSVTVTVSKH 416
V++++++ IEPLD S+I K V D+MK+TIS MLGLLPSD F V +
Sbjct: 74 RSPKKCVLSDLIQEIEPLDVSLIQKDVPVTTLDAMKRTISGMLGLLPSDRFQVHIESLWE 133
Query: 417 PLHRLLVSSIITGYTLWNAEYRMSLTRNLDIASPCGARDSDCEKRSEILEVKGGGEDGGE 596
PL +LLVSS++TGYTL NAEYR+ L +NLD++ G DS + +E +++G D
Sbjct: 134 PLSKLLVSSMMTGYTLRNAEYRLFLEKNLDMSG--GGLDSHASENTE-YDMEGTFPDEDH 190
Query: 597 IEVASDLGLKDLENCSSSPRVFGDLPPQALNYIQQLQS 710
+ D ++L + G + +A YI +LQS
Sbjct: 191 VSSKRDSRTQNLSE-TIDEEGLGRVSSEAQEYILRLQS 227
>gb|AAF21309.1| seed maturation protein PM23 [Glycine max]
Length = 404
Score = 94.0 bits (232), Expect = 2e-18
Identities = 67/179 (37%), Positives = 96/179 (53%), Gaps = 7/179 (3%)
Frame = +3
Query: 195 SSSSDDVVSTRKSTFDRGFTVIANMLRRIEPLDNSVISKGVSDAARDSMKQTISTMLGLL 374
++SS D S K + V+ +++ IEPLD S I K V D+MK+TIS MLGLL
Sbjct: 75 AASSHDFASNSKKS------VLTELIQEIEPLDVSHIQKDVPPTTADAMKRTISGMLGLL 128
Query: 375 PSDHFSVTVTVSKHPLHRLLVSSIITGYTLWNAEYRMSLTRNLDIASPCGARDSDCEK-R 551
PSD F V + PL +LL+SS++TGYTL N EYR+ L +NLD+ + D EK +
Sbjct: 129 PSDQFHVVIEALWEPLSKLLISSMMTGYTLRNVEYRLCLEKNLDMF------EGDIEKPK 182
Query: 552 SEILEVKGGG---EDGGEIEVASDLGLKD-LENCSSSPRV--FGDLPPQALNYIQQLQS 710
+E ++V G + IE + L +E + G++ +A YI LQS
Sbjct: 183 AESMKVDLQGLMHDSVNAIEFGKNKNLSSKVEKLHEEVDIQELGEISAEAQQYIFNLQS 241
>gb|ZP_00056820.1| hypothetical protein [Thermobifida fusca]
Length = 754
Score = 69.3 bits (168), Expect = 5e-11
Identities = 54/140 (38%), Positives = 72/140 (50%), Gaps = 9/140 (6%)
Frame = +1
Query: 61 PSSSHASPTSHPILSTHPSHHLSSSA-----AAAASSPPATPMGSRRPLLPHP---LTMS 216
PS+ AS P+ S PS + ++S A AASS T + P P P T S
Sbjct: 178 PSTQPASSPPEPLRSASPSKNSTNSPSPHPPAPAASSSCPTSTANAPPTCPTPPAASTAS 237
Query: 217 SPPGNPPSTAASPSSPTCSAASSPSITPSSQRVSP-TPPETP*SRPSPPCSVCSPPITSL 393
P +PP T +PSS CSAAS TPSS +VSP T + + P+PP S SPP +S
Sbjct: 238 PEPTSPPPTWPAPSSKACSAASLTPSTPSSTKVSPSTVSSSSAAAPAPPQSAPSPPKSSA 297
Query: 394 SLSLFPSTPSIVFSSLPSSP 453
+ S PS P ++ P++P
Sbjct: 298 APS--PSQPKA--NTSPTAP 313
Score = 60.8 bits (146), Expect = 2e-08
Identities = 48/141 (34%), Positives = 69/141 (48%), Gaps = 7/141 (4%)
Frame = +1
Query: 52 HG-DPSSSHASPTS-HPILSTHPSHHLSSSAAAAASSPPATPMGSRRPLLPHPLTMSSPP 225
HG PS+ SP S P + P+ + ++ ASS P T + P P+P ++ P
Sbjct: 5 HGAKPSAQSPSPASPSPNSAGSPNTNPTTPPEPKASSCPTTGSPNALPKPPNPSPTAATP 64
Query: 226 GNPPSTAA--SPSSPTCSAASSPSITPSSQRVSPTPPETP*SRP---SPPCSVCSPPITS 390
P +T +P+ PTCS SPS S+ R SP PP++ + P SPP + +P +
Sbjct: 65 PAPATTTPPPTPTGPTCSP--SPSAANSAPRESPPPPKSSAASPPPNSPPSTSSAPAPAT 122
Query: 391 LSLSLFPSTPSIVFSSLPSSP 453
PSTP SS PS+P
Sbjct: 123 TWPPHSPSTPDPATSSSPSAP 143
Score = 55.8 bits (133), Expect = 6e-07
Identities = 50/142 (35%), Positives = 65/142 (45%), Gaps = 1/142 (0%)
Frame = +1
Query: 31 PIPWQHSHGDPSSSHASPTSHPI-LSTHPSHHLSSSAAAAASSPPATPMGSRRPLLPHPL 207
P P + P +S S +S P +T P H S+ A +SSP A P P P P
Sbjct: 97 PPPKSSAASPPPNSPPSTSSAPAPATTWPPHSPSTPDPATSSSPSAPP----EPPSPSP- 151
Query: 208 TMSSPPGNPPSTAASPSSPTCSAASSPSITPSSQRVSPTPPETP*SRPSPPCSVCSPPIT 387
+PP PP A SP SPT ASSPS PS+Q S +PPE S S SP
Sbjct: 152 --KNPPPTPP--APSPDSPTPPDASSPSSAPSTQPAS-SPPEPLRSASPSKNSTNSPSPH 206
Query: 388 SLSLSLFPSTPSIVFSSLPSSP 453
+ + S P+ ++ P+ P
Sbjct: 207 PPAPAASSSCPTSTANAPPTCP 228
Score = 55.5 bits (132), Expect = 7e-07
Identities = 54/186 (29%), Positives = 80/186 (42%), Gaps = 23/186 (12%)
Frame = +1
Query: 1 CRALFLPSFLPIPWQHSHGDPSSSHASPTSHPILSTHPSHHLSSSAAAAASSPPATPMGS 180
C P+ LP P +PS + A+P + P +T P + + + S+ + P S
Sbjct: 42 CPTTGSPNALPKP-----PNPSPTAATPPA-PATTTPPPTPTGPTCSPSPSAANSAPRES 95
Query: 181 RRPLLPHPLTMSSPPGNPPSTAA-----------SPSSPTCSAASSPSI-----TPSSQR 312
P P S PP +PPST++ SPS+P + +SSPS +PS +
Sbjct: 96 PPP--PKSSAASPPPNSPPSTSSAPAPATTWPPHSPSTPDPATSSSPSAPPEPPSPSPKN 153
Query: 313 VSPTPPETP*SRPSPPCSVCSPPITSLSLSLFPSTPSIVFSSLPS-------SPGTLCGM 471
PTPP P+PP + S P ++ S S P + S+ PS SP
Sbjct: 154 PPPTPPAPSPDSPTPPDA--SSPSSAPSTQPASSPPEPLRSASPSKNSTNSPSPHPPAPA 211
Query: 472 RSTGCP 489
S+ CP
Sbjct: 212 ASSSCP 217
Score = 43.1 bits (100), Expect = 0.004
Identities = 35/101 (34%), Positives = 49/101 (47%), Gaps = 1/101 (0%)
Frame = +1
Query: 61 PSSSHASPTSHPILSTHPSHHLSSSAAAAASSPPATPMGSRRPLLPHPLTMSSPPGNPPS 240
P++S ASP T P+ SS A +AAS P+TP ++ P T+SS P+
Sbjct: 231 PAASTASPEPTSPPPTWPAP--SSKACSAASLTPSTPSSTK----VSPSTVSSSSAAAPA 284
Query: 241 TAAS-PSSPTCSAASSPSITPSSQRVSPTPPETP*SRPSPP 360
S PS P SAA SPS ++ + P + P P+ P
Sbjct: 285 PPQSAPSPPKSSAAPSPSQPKANTSPTAPPAKQPGHSPANP 325
Score = 40.4 bits (93), Expect = 0.024
Identities = 40/134 (29%), Positives = 54/134 (39%), Gaps = 9/134 (6%)
Frame = +1
Query: 61 PSSSHASPTSHPILSTHPSHHLSSSAAAAASSPPATPMGSRRPLLPHPLTMSSPPGNPPS 240
PSS+ SP++ +SSS+AAA + P + P + P P S P N
Sbjct: 265 PSSTKVSPST-----------VSSSSAAAPAPPQSAPSPPKSSAAPSP---SQPKANTSP 310
Query: 241 TAASPSSPTCSAASSP---SITPSSQRVSPTPPE-----TP*SRPSPPCSVCSPPIT-SL 393
TA P S A+ P TP PTPP TP +P + + P T
Sbjct: 311 TAPPAKQPGHSPANPPHPNGKTPEPSSAKPTPPRRYANATP--KPETDSNRKTKPTTKEA 368
Query: 394 SLSLFPSTPSIVFS 435
+S + TP FS
Sbjct: 369 HMSNYQPTPEDRFS 382
Score = 33.1 bits (74), Expect = 3.9
Identities = 28/97 (28%), Positives = 41/97 (41%)
Frame = +1
Query: 163 ATPMGSRRPLLPHPLTMSSPPGNPPSTAASPSSPTCSAASSPSITPSSQRVSPTPPETP* 342
A P G++ P P S P + S +P++P ASS T S + P +P
Sbjct: 2 APPHGAK-PSAQSPSPASPSPNSAGSPNTNPTTPPEPKASSCPTTGSPNALPKPPNPSPT 60
Query: 343 SRPSPPCSVCSPPITSLSLSLFPSTPSIVFSSLPSSP 453
+ P + +PP T + PS PS S+ SP
Sbjct: 61 AATPPAPATTTPPPTPTGPTCSPS-PSAANSAPRESP 96
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 762,837,119
Number of Sequences: 1393205
Number of extensions: 21681379
Number of successful extensions: 294074
Number of sequences better than 10.0: 7497
Number of HSP's better than 10.0 without gapping: 128444
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 225390
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 32654539052
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
