>KMC018001A_C01 KMC018001A_c01
gatctaaacatattatAACACAAAAAAAGTAAAGGGTTACATAAAAGTGGTAATGAACTG
TCTCCTGCTGTTCGTAGAACAGCAAATGAAATTTGGGAGGCAAAACTTAGCTACCAAGTT
CATCACAACAATGTTTATGCCCCGTGTATGAAAATTTAAGGAGGATAGCATTACTCATAT
CGCCCGATCAAATGGTAGGCATCACTATGACAAATCAGAGCTACAGATTACATTAAAAGC
AATAGCAGAAACGAGGCAACCAAAGGGAAAAACTTCAATGACTTCCTATTGACCGATGCT
TACGAGTAAAAATTATGCCTGTGAGTTAAGGATGCGGATGTGTATCTCAGAGAATGTCTT
CACATCAGTACCCAATAACCAGCAATCTATCCCCACAGCATTCGCACCGAATTTATCTGC
CTTTTCATCATCTCCAATGTGTATGGCTTTGGAAGCCTCTACATTTAGTTGATCTAGAGC
TGCTTGAAATATTCTTGGGTCTGGTTTTTCATATTCAACCTCTG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC018001A_C01 KMC018001A_c01
         (524 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAO42097.1| unknown protein [Arabidopsis thaliana]                  95  6e-19
pir||C86277 F14L17.7 protein - Arabidopsis thaliana gi|7262672|g...    95  6e-19
ref|NP_172883.1| unknown protein; protein id: At1g14310.1 [Arabi...    86  2e-16
dbj|BAC15484.1| contains ESTs AU091627(C0596),D15410(C0596A)~sim...    64  8e-10
ref|NP_181658.1| hypothetical protein; protein id: At2g41250.1, ...    64  1e-09

>gb|AAO42097.1| unknown protein [Arabidopsis thaliana]
          Length = 250

 Score = 94.7 bits (234), Expect = 6e-19
 Identities = 43/68 (63%), Positives = 57/68 (83%)
 Frame = -3

Query: 522 EVEYEKPDPRIFQAALDQLNVEASKAIHIGDDEKADKFGANAVGIDCWLLGTDVKTFSEI 343
           EV YEKPD RIF++AL+Q++V+ ++A+H+GDDE ADK GANA+GI CWL G DV+TFS+I
Sbjct: 182 EVGYEKPDERIFKSALEQISVDVNRAVHVGDDEGADKGGANAIGIACWLWGEDVQTFSDI 241

Query: 342 HIRILNSQ 319
             RIL S+
Sbjct: 242 QKRILVSE 249

>pir||C86277 F14L17.7 protein - Arabidopsis thaliana
           gi|7262672|gb|AAF43930.1|AC012188_7 Contains similarity
           to a hypothetical protein from Arabidopsis thaliana
           gb|AC005662.2 and contains a haloacid dehalogenase-like
           hydrolase PF|00702 domain.  EST gb|F15167 comes from
           this gene
          Length = 254

 Score = 94.7 bits (234), Expect = 6e-19
 Identities = 43/68 (63%), Positives = 57/68 (83%)
 Frame = -3

Query: 522 EVEYEKPDPRIFQAALDQLNVEASKAIHIGDDEKADKFGANAVGIDCWLLGTDVKTFSEI 343
           EV YEKPD RIF++AL+Q++V+ ++A+H+GDDE ADK GANA+GI CWL G DV+TFS+I
Sbjct: 186 EVGYEKPDERIFKSALEQISVDVNRAVHVGDDEGADKGGANAIGIACWLWGEDVQTFSDI 245

Query: 342 HIRILNSQ 319
             RIL S+
Sbjct: 246 QKRILVSE 253

>ref|NP_172883.1| unknown protein; protein id: At1g14310.1 [Arabidopsis thaliana]
          Length = 265

 Score = 86.3 bits (212), Expect = 2e-16
 Identities = 43/79 (54%), Positives = 57/79 (71%), Gaps = 11/79 (13%)
 Frame = -3

Query: 522 EVEYEKPDPRIFQAAL-----------DQLNVEASKAIHIGDDEKADKFGANAVGIDCWL 376
           EV YEKPD RIF++AL           +Q++V+ ++A+H+GDDE ADK GANA+GI CWL
Sbjct: 186 EVGYEKPDERIFKSALGIGILHSKLNAEQISVDVNRAVHVGDDEGADKGGANAIGIACWL 245

Query: 375 LGTDVKTFSEIHIRILNSQ 319
            G DV+TFS+I  RIL S+
Sbjct: 246 WGEDVQTFSDIQKRILVSE 264

>dbj|BAC15484.1| contains ESTs AU091627(C0596),D15410(C0596A)~similar to Arabidopsis
           thaliana chromosome 2, At2g41250~unknown protein [Oryza
           sativa (japonica cultivar-group)]
           gi|22831190|dbj|BAC16048.1| P0496C02.7 [Oryza sativa
           (japonica cultivar-group)]
          Length = 304

 Score = 64.3 bits (155), Expect = 8e-10
 Identities = 31/67 (46%), Positives = 41/67 (60%)
 Frame = -3

Query: 522 EVEYEKPDPRIFQAALDQLNVEASKAIHIGDDEKADKFGANAVGIDCWLLGTDVKTFSEI 343
           EV  EKP+P IF  A + L V+  +A+HIGDD + D +GA   G D WL G+DV +F E 
Sbjct: 215 EVAAEKPNPTIFLKACEFLGVKPEEAVHIGDDRRNDLWGARDAGCDAWLWGSDVYSFKEN 274

Query: 342 HIRILNS 322
              I+ S
Sbjct: 275 QAHIIKS 281

>ref|NP_181658.1| hypothetical protein; protein id: At2g41250.1, supported by cDNA:
           gi_17979160 [Arabidopsis thaliana]
           gi|25408736|pir||F84839 hypothetical protein At2g41250
           [imported] - Arabidopsis thaliana
           gi|3894197|gb|AAC78546.1| hypothetical protein
           [Arabidopsis thaliana] gi|17979161|gb|AAL49776.1|
           unknown protein [Arabidopsis thaliana]
           gi|22136692|gb|AAM91665.1| unknown protein [Arabidopsis
           thaliana]
          Length = 290

 Score = 63.5 bits (153), Expect = 1e-09
 Identities = 29/64 (45%), Positives = 40/64 (62%)
 Frame = -3

Query: 522 EVEYEKPDPRIFQAALDQLNVEASKAIHIGDDEKADKFGANAVGIDCWLLGTDVKTFSEI 343
           EVE EKP+P IF  A + L V    A+H+GDD + D +GA   G D WL G++V +F ++
Sbjct: 223 EVEAEKPNPTIFLKACELLEVNPEDAVHVGDDRRNDVWGARDAGCDAWLWGSEVTSFKQV 282

Query: 342 HIRI 331
             RI
Sbjct: 283 AQRI 286

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 425,980,196
Number of Sequences: 1393205
Number of extensions: 8325604
Number of successful extensions: 19395
Number of sequences better than 10.0: 184
Number of HSP's better than 10.0 without gapping: 18958
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19370
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17019769648
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPD013b11_f BP045013 1 519 2 MFB083f08_f BP040086 17 433 3 SPD077g02_f BP050183 17 525

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