>KMC017725A_C01 KMC017725A_c01
agaaAAAGTGAAGACAGATAAAGTTTCAGTGGCGAGGGTGCTGGTGTTGTTTTGAGCAAT
CACCAACCATGAAAAACCATTCAATAGTAGCAAAGCAAGAACAACCCAATCGAAAGGGAG
GTATATTTGAACCTCACAGAGCAATCGGATGCATCACCACCGGCGTCCCCTTCTCCATTC
AGAGACTCGGCACCGAAACTTTCGTCACTGTCAGCGTCGGTAAAGCTTTTCAGATTTTCA
ACTGTGCAAAGCTCACTCTGCTCCTCGCTGGTCCTTTACTGCCGAAAAAGATTACTGCTC
TTGCCTCTTATCGTGATTACACTTTTGCTGCATATGGAACCAACATTGGAGTTTTCAAGC
GCGCGCATCAGGTTGCCACTTGGAGCAGCCATAGCGCCAAGGTTAAATTGCTGCTGGTGT
TTGGAGACCATGTTATTAGTGTTGATGCTGATGGCGACATGTTCATGTGGGCGTTTAAGG
GGAATGAAGAGAGCAATGTTGTCCCGTTTAAGCATATTAAGCTAGATGAGAAATTTAGCC
CTAGCTGTATAATGCATCCAGATACTTACATTAATAAGGTTC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC017725A_C01 KMC017725A_c01
         (582 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_567275.1| putative WD-repeat membrane protein; protein id...   224  5e-58
pir||B85062 probable WD-repeat membrane protein [imported] - Ara...   219  3e-56
ref|NP_644810.1| T-cell activation WD repeat protein; similar to...   102  3e-21
ref|NP_587878.1| beta transducin [Schizosaccharomyces pombe] gi|...    94  2e-18
dbj|BAC04527.1| unnamed protein product [Homo sapiens]                 92  4e-18

>ref|NP_567275.1| putative WD-repeat membrane protein; protein id: At4g04940.1,
           supported by cDNA: gi_16604678, supported by cDNA:
           gi_20465602 [Arabidopsis thaliana]
           gi|16604679|gb|AAL24132.1| putative WD-repeat membrane
           protein [Arabidopsis thaliana]
           gi|20465603|gb|AAM20284.1| putative WD-repeat membrane
           protein [Arabidopsis thaliana]
          Length = 910

 Score =  224 bits (572), Expect = 5e-58
 Identities = 108/154 (70%), Positives = 130/154 (84%)
 Frame = +3

Query: 120 GIFEPHRAIGCITTGVPFSIQRLGTETFVTVSVGKAFQIFNCAKLTLLLAGPLLPKKITA 299
           GIFEP RAIG IT+ VPFS+QRLGTETFVTVSVGKAFQI+NCAKL L++  P LPKKI A
Sbjct: 2   GIFEPFRAIGYITSTVPFSVQRLGTETFVTVSVGKAFQIYNCAKLNLVIISPQLPKKIRA 61

Query: 300 LASYRDYTFAAYGTNIGVFKRAHQVATWSSHSAKVKLLLVFGDHVISVDADGDMFMWAFK 479
           LASYRDYTF A+G  I VF+RAHQVATWS H AKV LLLVFG+HV+S+D +G+MF+WAFK
Sbjct: 62  LASYRDYTFVAFGNEIAVFRRAHQVATWSKHVAKVDLLLVFGEHVLSLDVEGNMFIWAFK 121

Query: 480 GNEESNVVPFKHIKLDEKFSPSCIMHPDTYINKV 581
           G EE ++ P  +++L  KF+P+ I+HPDTY+NKV
Sbjct: 122 GIEE-HLAPIGNLQLTGKFTPTSIVHPDTYLNKV 154

>pir||B85062 probable WD-repeat membrane protein [imported] - Arabidopsis
           thaliana gi|4325344|gb|AAD17343.1| similar to
           beta-transducins (Pfam: PF00400, Score=71.7, E=1.5e-17,
           N=6) [Arabidopsis thaliana] gi|7267253|emb|CAB81036.1|
           putative WD-repeat membrane protein [Arabidopsis
           thaliana]
          Length = 931

 Score =  219 bits (557), Expect = 3e-56
 Identities = 110/161 (68%), Positives = 130/161 (80%), Gaps = 7/161 (4%)
 Frame = +3

Query: 120 GIFEPHRAIGCITTGVPFSIQRLGTETFVTVSVGKAFQIFNCAKLTL-------LLAGPL 278
           GIFEP RAIG IT+ VPFS+QRLGTETFVTVSVGKAFQI+NCAKL L       LL  P 
Sbjct: 2   GIFEPFRAIGYITSTVPFSVQRLGTETFVTVSVGKAFQIYNCAKLNLVIISKFFLLLKPK 61

Query: 279 LPKKITALASYRDYTFAAYGTNIGVFKRAHQVATWSSHSAKVKLLLVFGDHVISVDADGD 458
           LPKKI ALASYRDYTF A+G  I VF+RAHQVATWS H AKV LLLVFG+HV+S+D +G+
Sbjct: 62  LPKKIRALASYRDYTFVAFGNEIAVFRRAHQVATWSKHVAKVDLLLVFGEHVLSLDVEGN 121

Query: 459 MFMWAFKGNEESNVVPFKHIKLDEKFSPSCIMHPDTYINKV 581
           MF+WAFKG EE ++ P  +++L  KF+P+ I+HPDTY+NKV
Sbjct: 122 MFIWAFKGIEE-HLAPIGNLQLTGKFTPTSIVHPDTYLNKV 161

>ref|NP_644810.1| T-cell activation WD repeat protein; similar to SD06427p [Homo
           sapiens] gi|21205868|gb|AAM43838.1|AF385437_1 T-cell
           activation WD repeat protein; TA-WDRP [Homo sapiens]
          Length = 951

 Score =  102 bits (255), Expect = 3e-21
 Identities = 59/190 (31%), Positives = 95/190 (49%), Gaps = 7/190 (3%)
 Frame = +3

Query: 33  RGCWCCFEQSPTMKNHSIVAKQEQPNRK---GGIFEPHRAIGCITTGVPFSIQ--RLGTE 197
           +G   CF   P ++   I A  E+ + +     +F   RA+G  +  +P  ++   L   
Sbjct: 36  KGLGTCFPSGPELRGAGIAAAMERASERRTASALFAGFRALGLFSNDIPHVVRFSALKRR 95

Query: 198 TFVTVSVGKAFQIFNCAKLTLLLAGPLLPKKITALASYRDYTFAAYGTNIGVFKRAHQVA 377
            +VT  VGK+F  ++  KL+L+     +P+ I  +A+     FAAYG     F R  ++ 
Sbjct: 96  FYVTTCVGKSFHTYDVQKLSLVAVSNSVPQDICCMAADGRLVFAAYGNVFSAFARNKEIV 155

Query: 378 -TWSSHSAKVKLLLVFGDHVISVDADGDMFMWAFKGNEESNVVPFKHIKLDEK-FSPSCI 551
            T+  H A++  L  FGDH+ISVD DG + +W     EE     +  +  D+  F  S I
Sbjct: 156 HTFKGHKAEIHFLQPFGDHIISVDTDGILIIWHIYSEEE-----YLQLTFDKSVFKISAI 210

Query: 552 MHPDTYINKV 581
           +HP TY+NK+
Sbjct: 211 LHPSTYLNKI 220

>ref|NP_587878.1| beta transducin [Schizosaccharomyces pombe]
           gi|3219965|sp|O14053|YC47_SCHPO Hypothetical WD-repeat
           protein C1672.07 in chromosome III
           gi|7490150|pir||T41051 beta transducin - fission yeast
           (Schizosaccharomyces pombe) gi|3451308|emb|CAA20445.1|
           beta transducin [Schizosaccharomyces pombe]
          Length = 902

 Score = 93.6 bits (231), Expect = 2e-18
 Identities = 55/166 (33%), Positives = 84/166 (50%), Gaps = 6/166 (3%)
 Frame = +3

Query: 102 QPNRKGGIFEPHRAIGCITTGVPFSIQRLGTETFVTVSVGKAFQIFNCAKLTLLLAGPLL 281
           +P RK  I+ P R+IG ++  VPF I+  GT   VT SVG  FQ ++C KL LL  G  L
Sbjct: 16  KPLRKSRIYAPFRSIGHVSNAVPFDIEARGTHFLVTTSVGNTFQTYDCEKLNLLFVGKQL 75

Query: 282 PKKITALASYRDYTFAAYGTNIGVFKRAHQVATW----SSHSAKVKLLLVFGDHVISVDA 449
            K+IT L S++D+   A G+ I  +KR   +  W          V  L  FG+ +I+  +
Sbjct: 76  DKEITCLKSFKDFMLVAAGSKIFAYKRGKII--WDIDVEQEHGTVTHLDAFGEWIIACTS 133

Query: 450 DGDMFMWAFKGNEESNVVPFKHIKL--DEKFSPSCIMHPDTYINKV 581
              +++W    +     VP  H     +     + ++HP TY+NK+
Sbjct: 134 SRHVYVWK---HASKYSVPELHTTFLPNTNADITSLLHPSTYLNKI 176

>dbj|BAC04527.1| unnamed protein product [Homo sapiens]
          Length = 895

 Score = 92.4 bits (228), Expect = 4e-18
 Identities = 52/157 (33%), Positives = 82/157 (52%), Gaps = 4/157 (2%)
 Frame = +3

Query: 123 IFEPHRAIGCITTGVPFSIQ--RLGTETFVTVSVGKAFQIFNCAKLTLLLAGPLLPKKIT 296
           +F   RA+G  +  +P  ++   L    +VT  VGK+F  ++  KL+L+     +P+ I 
Sbjct: 13  LFAGFRALGLFSNDIPHVVRFSALKRRFYVTTCVGKSFHTYDVQKLSLVAVSNSVPQDIC 72

Query: 297 ALASYRDYTFAAYGTNIGVFKRAHQVA-TWSSHSAKVKLLLVFGDHVISVDADGDMFMWA 473
            +A+     FAAYG     F R  ++  T+  H A++  L  FGDH+ISVD DG + +W 
Sbjct: 73  CMAADGRLVFAAYGNVFSAFARNKEIVHTFKGHKAEIHFLQPFGDHIISVDTDGILIIWH 132

Query: 474 FKGNEESNVVPFKHIKLDEK-FSPSCIMHPDTYINKV 581
               EE     +  +  D+  F  S I+HP TY+NK+
Sbjct: 133 IYSEEE-----YLQLTFDKSVFKISAILHPSTYLNKI 164

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 494,903,588
Number of Sequences: 1393205
Number of extensions: 10603685
Number of successful extensions: 37698
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 35876
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37630
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21712003912
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPDL083h01_f BP057218 1 510 2 MFBL043f09_f BP043458 5 582

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