Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC017575A_C01 KMC017575A_c01
(562 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T46574 dnaK-type molecular chaperone BiP precursor [similar... 204 8e-52
pir||T06358 dnaK-type molecular chapreone BiP-B - soybean gi|475... 201 4e-51
sp|Q03682|BIP2_TOBAC Luminal binding protein 2 (BiP 2) (78 kDa g... 197 7e-50
sp|P49118|BIP_LYCES Luminal binding protein precursor (BiP) (78 ... 196 1e-49
sp|Q03685|BIP5_TOBAC Luminal binding protein 5 precursor (BiP 5)... 196 1e-49
>pir||T46574 dnaK-type molecular chaperone BiP precursor [similarity] - soybean
gi|2642238|gb|AAB86942.1| endoplasmic reticulum
HSC70-cognate binding protein precursor [Glycine max]
Length = 668
Score = 204 bits (518), Expect = 8e-52
Identities = 101/116 (87%), Positives = 111/116 (95%)
Frame = -2
Query: 561 EEFAEEDKKVKERIDARHGLETYIYNMKNQVSEKDKLADQLESDEKEKIETAVKEALEWL 382
++FAEE+KKVKERIDAR+ LETY+YNMKNQVS+KDKLAD+LESDEKEKIETAVKEALEWL
Sbjct: 554 KDFAEEEKKVKERIDARNSLETYVYNMKNQVSDKDKLADKLESDEKEKIETAVKEALEWL 613
Query: 381 DDHPSAEKEEYEEKLKEVEAVCNPIITAVYQRSGGAPGGEGASGEGEDEDDSHDEL 214
DD+ S EKE+YEEKLKEVEAVCNPII+AVYQRSGGAPGG GASGE EDEDDSHDEL
Sbjct: 614 DDNQSMEKEDYEEKLKEVEAVCNPIISAVYQRSGGAPGGGGASGE-EDEDDSHDEL 668
>pir||T06358 dnaK-type molecular chapreone BiP-B - soybean
gi|475600|gb|AAA81954.1| BiP isoform B
Length = 666
Score = 201 bits (512), Expect = 4e-51
Identities = 102/116 (87%), Positives = 110/116 (93%)
Frame = -2
Query: 561 EEFAEEDKKVKERIDARHGLETYIYNMKNQVSEKDKLADQLESDEKEKIETAVKEALEWL 382
EEFAEEDKKVKERIDAR+ LETY+YNMKNQ+S+KDKLAD+LESDEKEKIETAVKEALEWL
Sbjct: 553 EEFAEEDKKVKERIDARNSLETYVYNMKNQISDKDKLADKLESDEKEKIETAVKEALEWL 612
Query: 381 DDHPSAEKEEYEEKLKEVEAVCNPIITAVYQRSGGAPGGEGASGEGEDEDDSHDEL 214
DD+ S EKE+YEEKLKE EAVCNPII+AVYQRSGGAPGG GASGE EDEDDSHDEL
Sbjct: 613 DDNQSMEKEDYEEKLKE-EAVCNPIISAVYQRSGGAPGGGGASGE-EDEDDSHDEL 666
>sp|Q03682|BIP2_TOBAC Luminal binding protein 2 (BiP 2) (78 kDa glucose-regulated protein
homolog 2) (GRP 78-2) gi|100338|pir||S21878 dnaK-type
molecular chaperone blp2 - common tobacco (fragment)
gi|19807|emb|CAA42661.1| luminal binding protein (BiP)
[Nicotiana tabacum]
Length = 292
Score = 197 bits (501), Expect = 7e-50
Identities = 97/116 (83%), Positives = 107/116 (91%)
Frame = -2
Query: 561 EEFAEEDKKVKERIDARHGLETYIYNMKNQVSEKDKLADQLESDEKEKIETAVKEALEWL 382
EEFAEEDKKVKERIDAR+ LETY+YNMKNQ+++KDKLAD+LESDEKEKIETA KEALEWL
Sbjct: 179 EEFAEEDKKVKERIDARNSLETYVYNMKNQINDKDKLADKLESDEKEKIETATKEALEWL 238
Query: 381 DDHPSAEKEEYEEKLKEVEAVCNPIITAVYQRSGGAPGGEGASGEGEDEDDSHDEL 214
DD+ SAEKE+YEEKLKEVEAVCNPIITAVYQRSGGAPG G S E E+E+D HDEL
Sbjct: 239 DDNQSAEKEDYEEKLKEVEAVCNPIITAVYQRSGGAPG--GGSSEEEEEEDGHDEL 292
>sp|P49118|BIP_LYCES Luminal binding protein precursor (BiP) (78 kDa glucose-regulated
protein homolog) (GRP 78) gi|170384|gb|AAA34139.1|
glucose-regulated protein 78
Length = 666
Score = 196 bits (499), Expect = 1e-49
Identities = 96/116 (82%), Positives = 107/116 (91%)
Frame = -2
Query: 561 EEFAEEDKKVKERIDARHGLETYIYNMKNQVSEKDKLADQLESDEKEKIETAVKEALEWL 382
EEFAEEDKKVKE+IDAR+ LETY+YNMKNQ+++KDKLAD+LESDEKEKIETA KEALEWL
Sbjct: 555 EEFAEEDKKVKEKIDARNALETYVYNMKNQINDKDKLADKLESDEKEKIETATKEALEWL 614
Query: 381 DDHPSAEKEEYEEKLKEVEAVCNPIITAVYQRSGGAPGGEGASGEGEDEDDSHDEL 214
DD+ SAEKE+Y+EKLKEVEAVCNPIITAVYQRSGGAPGG G E+EDDSHDEL
Sbjct: 615 DDNQSAEKEDYDEKLKEVEAVCNPIITAVYQRSGGAPGG----GASEEEDDSHDEL 666
>sp|Q03685|BIP5_TOBAC Luminal binding protein 5 precursor (BiP 5) (78 kDa
glucose-regulated protein homolog 5) (GRP 78-5)
gi|100340|pir||S21880 dnaK-type molecular chaperone blp5
precursor - common tobacco gi|19813|emb|CAA42660.1|
luminal binding protein (BiP) [Nicotiana tabacum]
Length = 668
Score = 196 bits (499), Expect = 1e-49
Identities = 96/116 (82%), Positives = 108/116 (92%)
Frame = -2
Query: 561 EEFAEEDKKVKERIDARHGLETYIYNMKNQVSEKDKLADQLESDEKEKIETAVKEALEWL 382
EEFAEEDKKVKERIDAR+ LETY+YNM+NQ+++KDKLAD+LESDEKEKIETA KEALEWL
Sbjct: 555 EEFAEEDKKVKERIDARNSLETYVYNMRNQINDKDKLADKLESDEKEKIETATKEALEWL 614
Query: 381 DDHPSAEKEEYEEKLKEVEAVCNPIITAVYQRSGGAPGGEGASGEGEDEDDSHDEL 214
DD+ SAEKE+Y+EKLKEVEAVCNPIITAVYQRSGGAPG GAS E ++DDSHDEL
Sbjct: 615 DDNQSAEKEDYDEKLKEVEAVCNPIITAVYQRSGGAPG--GASEESNEDDDSHDEL 668
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 502,279,060
Number of Sequences: 1393205
Number of extensions: 11759202
Number of successful extensions: 70246
Number of sequences better than 10.0: 1354
Number of HSP's better than 10.0 without gapping: 54472
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 67396
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20095422690
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
