Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC017232A_C01 KMC017232A_c01
(650 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAC24001.1| isoflavone reductase related protein [Pyrus commu... 266 2e-70
ref|NP_195634.1| isoflavone reductase, putative; protein id: At4... 259 3e-68
gb|AAL85023.1| putative NAD(P)H oxidoreductase, isoflavone reduc... 257 8e-68
gb|AAF17578.1|AF202184_1 isoflavone reductase homolog 2 [Glycine... 254 9e-67
gb|AAG22740.1|AF282850_1 allergenic isoflavone reductase-like pr... 250 1e-65
>gb|AAC24001.1| isoflavone reductase related protein [Pyrus communis]
Length = 308
Score = 266 bits (679), Expect = 2e-70
Identities = 127/162 (78%), Positives = 147/162 (90%)
Frame = -1
Query: 650 HTIVSCNFFASYFLSTLSQPGVTTPPRDKVVILGDGNPKCVFNKEDDIGTYTIRAVSDPR 471
+T VS NFFA YFL TL+QPG ++ PRDKVVILGDGNPK +FNKEDDIGTYTIRAV DPR
Sbjct: 147 YTYVSSNFFAGYFLPTLNQPGASSAPRDKVVILGDGNPKAIFNKEDDIGTYTIRAVDDPR 206
Query: 470 TLNKILYIRPPANTLSFNDLVSLWEKKIGKTLERVYVPEDQVLKQIKESPVPNSVMLAIS 291
TLNK+LYIRPPANT+SFN+LVSLWEKKIGKTLER+YVPE+Q+LK I+E+ VP +V+L+IS
Sbjct: 207 TLNKVLYIRPPANTISFNELVSLWEKKIGKTLERIYVPEEQLLKNIQEAAVPLNVILSIS 266
Query: 290 HAVYVKGDQTYFEIEQSFGVEASSLYPDVKYTTVDELLDQFV 165
HAV+VKGD T FEIE SFGVEA++LYPDVKYTTVDE L+QFV
Sbjct: 267 HAVFVKGDHTNFEIEPSFGVEATALYPDVKYTTVDEYLNQFV 308
>ref|NP_195634.1| isoflavone reductase, putative; protein id: At4g39230.1, supported
by cDNA: gi_19310584 [Arabidopsis thaliana]
gi|7484776|pir||T08571 2'-hydroxyisoflavone reductase
(EC 1.3.1.45) T22F8.130 - Arabidopsis thaliana
gi|4914435|emb|CAB43638.1| NAD(P)H oxidoreductase,
isoflavone reductase-like protein [Arabidopsis thaliana]
gi|7270906|emb|CAB80586.1| NAD(P)H oxidoreductase,
isoflavone reductase-like protein [Arabidopsis thaliana]
gi|23297378|gb|AAN12954.1| putative NAD(P)H
oxidoreductase, isoflavone reductase [Arabidopsis
thaliana]
Length = 308
Score = 259 bits (661), Expect = 3e-68
Identities = 124/162 (76%), Positives = 140/162 (85%)
Frame = -1
Query: 650 HTIVSCNFFASYFLSTLSQPGVTTPPRDKVVILGDGNPKCVFNKEDDIGTYTIRAVSDPR 471
+T VSCNFFA YFL TL+QPG T+ PRDKV++LGDGNPK VFNKE+DIGTYTI AV DPR
Sbjct: 147 YTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLGDGNPKAVFNKEEDIGTYTINAVDDPR 206
Query: 470 TLNKILYIRPPANTLSFNDLVSLWEKKIGKTLERVYVPEDQVLKQIKESPVPNSVMLAIS 291
TLNKILYIRPP NT SFNDLVSLWE KIGKTLER+YVPE+Q+LKQI ES P +VML++
Sbjct: 207 TLNKILYIRPPMNTYSFNDLVSLWENKIGKTLERIYVPEEQLLKQIIESSPPLNVMLSLC 266
Query: 290 HAVYVKGDQTYFEIEQSFGVEASSLYPDVKYTTVDELLDQFV 165
H V+VKG T FEIE SFGVEAS LYPDVKYTTVDE+L+Q+V
Sbjct: 267 HCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTVDEILNQYV 308
>gb|AAL85023.1| putative NAD(P)H oxidoreductase, isoflavone reductase [Arabidopsis
thaliana]
Length = 308
Score = 257 bits (657), Expect = 8e-68
Identities = 123/162 (75%), Positives = 140/162 (85%)
Frame = -1
Query: 650 HTIVSCNFFASYFLSTLSQPGVTTPPRDKVVILGDGNPKCVFNKEDDIGTYTIRAVSDPR 471
+T VSCNFFA YFL TL++PG T+ PRDKV++LGDGNPK VFNKE+DIGTYTI AV DPR
Sbjct: 147 YTYVSCNFFAGYFLPTLARPGATSAPRDKVIVLGDGNPKAVFNKEEDIGTYTINAVDDPR 206
Query: 470 TLNKILYIRPPANTLSFNDLVSLWEKKIGKTLERVYVPEDQVLKQIKESPVPNSVMLAIS 291
TLNKILYIRPP NT SFNDLVSLWE KIGKTLER+YVPE+Q+LKQI ES P +VML++
Sbjct: 207 TLNKILYIRPPMNTYSFNDLVSLWENKIGKTLERIYVPEEQLLKQIIESSPPLNVMLSLC 266
Query: 290 HAVYVKGDQTYFEIEQSFGVEASSLYPDVKYTTVDELLDQFV 165
H V+VKG T FEIE SFGVEAS LYPDVKYTTVDE+L+Q+V
Sbjct: 267 HCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTVDEILNQYV 308
>gb|AAF17578.1|AF202184_1 isoflavone reductase homolog 2 [Glycine max]
Length = 310
Score = 254 bits (648), Expect = 9e-67
Identities = 123/161 (76%), Positives = 140/161 (86%)
Frame = -1
Query: 647 TIVSCNFFASYFLSTLSQPGVTTPPRDKVVILGDGNPKCVFNKEDDIGTYTIRAVSDPRT 468
T VS NFFA YFL LSQPG T PRD+V+ILGDGNPK VFNKE+DIGTYTI +V DPRT
Sbjct: 150 TYVSSNFFAGYFLPNLSQPGATAAPRDRVIILGDGNPKAVFNKEEDIGTYTINSVDDPRT 209
Query: 467 LNKILYIRPPANTLSFNDLVSLWEKKIGKTLERVYVPEDQVLKQIKESPVPNSVMLAISH 288
LNKILYIRPPANTLSFN+LV+LWE KIGKTLER+YVPE+Q+LKQI+ES P +V+L+I+H
Sbjct: 210 LNKILYIRPPANTLSFNELVTLWEGKIGKTLERIYVPEEQLLKQIEESAPPVNVILSINH 269
Query: 287 AVYVKGDQTYFEIEQSFGVEASSLYPDVKYTTVDELLDQFV 165
+ YVKGD T FEIE SFGVEAS+LYPDVKY TVDE L+QFV
Sbjct: 270 SSYVKGDHTNFEIESSFGVEASALYPDVKYITVDEYLNQFV 310
>gb|AAG22740.1|AF282850_1 allergenic isoflavone reductase-like protein Bet v 6.0102 [Betula
pendula]
Length = 308
Score = 250 bits (639), Expect = 1e-65
Identities = 118/162 (72%), Positives = 142/162 (86%)
Frame = -1
Query: 650 HTIVSCNFFASYFLSTLSQPGVTTPPRDKVVILGDGNPKCVFNKEDDIGTYTIRAVSDPR 471
+T VS NFFA YFL TL+QPG+T+PPR+KVVI GDGN + VFNKEDDIGTYTIRAV DPR
Sbjct: 147 YTYVSSNFFAGYFLPTLAQPGLTSPPREKVVIFGDGNARAVFNKEDDIGTYTIRAVDDPR 206
Query: 470 TLNKILYIRPPANTLSFNDLVSLWEKKIGKTLERVYVPEDQVLKQIKESPVPNSVMLAIS 291
TLNKI+YI+P N SFN++V+LWEKKIGKTLE++YVPE+++LK I+ESP+P +V+LAI+
Sbjct: 207 TLNKIVYIKPAKNIYSFNEIVALWEKKIGKTLEKIYVPEEKLLKDIQESPIPINVILAIN 266
Query: 290 HAVYVKGDQTYFEIEQSFGVEASSLYPDVKYTTVDELLDQFV 165
H+V+VKGD T FEIE SFGVEAS LYPDVKYTTV+E L QFV
Sbjct: 267 HSVFVKGDHTNFEIEASFGVEASELYPDVKYTTVEEYLQQFV 308
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 552,394,990
Number of Sequences: 1393205
Number of extensions: 11616198
Number of successful extensions: 27274
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 26564
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27222
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 27860523586
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
