Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC016807A_C01 KMC016807A_c01
(585 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|ZP_00074000.1| hypothetical protein [Trichodesmium erythraeu... 50 3e-05
sp|P74476|RF2_SYNY3 Peptide chain release factor 2 (RF-2) gi|742... 47 1e-04
ref|NP_441899.1| peptide chain release factor [Synechocystis sp.... 47 1e-04
ref|NP_563086.1| peptide chain release factor 2 [Clostridium per... 47 2e-04
ref|NP_781032.1| bacterial peptide chain release factor 2 [Clost... 47 2e-04
>ref|ZP_00074000.1| hypothetical protein [Trichodesmium erythraeum IMS101]
Length = 246
Score = 49.7 bits (117), Expect = 3e-05
Identities = 32/104 (30%), Positives = 49/104 (46%)
Frame = -3
Query: 421 DTCGAKKIIIKDAAAPNYTSRKPSK*VAEIF*VKPGPLN*QTSKEHEQLEVMPLLEKSMD 242
D G K ++ Y K K + + P N + +EVMP+L+ S++
Sbjct: 44 DEAGLKSATLEIIGRYAYGYLKAEKGTHRLVRISPFNANGKRQTSFAGVEVMPILDHSVE 103
Query: 241 TERLGPGN*FFNSREGGKGGQIVDKVEIAVQITHNQTGVTIH*T 110
+ SR GGKGGQ V+KVE AV++ H TG+++ T
Sbjct: 104 LDIPEKDLEITTSRSGGKGGQNVNKVETAVRVVHIPTGLSVRCT 147
>sp|P74476|RF2_SYNY3 Peptide chain release factor 2 (RF-2) gi|7428986|pir||S76448
translation releasing factor RF-2 - Synechocystis sp.
(strain PCC 6803)
Length = 372
Score = 47.4 bits (111), Expect = 1e-04
Identities = 34/105 (32%), Positives = 49/105 (46%), Gaps = 1/105 (0%)
Frame = -3
Query: 421 DTCGAKKIIIKDAAAPNYTSRKPSK*VAEIF*VKPGPLN*QTSKEHEQLEVMPLL-EKSM 245
D G K + ++ Y K K + + P N + +EVMPLL E+++
Sbjct: 173 DEAGLKSVTLEIEGRYAYGYLKSEKGTHRLVRISPFNANGKRQTSFAGVEVMPLLGEEAI 232
Query: 244 DTERLGPGN*FFNSREGGKGGQIVDKVEIAVQITHNQTGVTIH*T 110
+ SR GGKGGQ V+KVE AV+I H TG+ + T
Sbjct: 233 SLDIPDKDLDISTSRAGGKGGQNVNKVETAVRIVHLPTGLAVRCT 277
>ref|NP_441899.1| peptide chain release factor [Synechocystis sp. PCC 6803]
gi|1653665|dbj|BAA18577.1| peptide chain release factor
[Synechocystis sp. PCC 6803]
Length = 288
Score = 47.4 bits (111), Expect = 1e-04
Identities = 34/105 (32%), Positives = 49/105 (46%), Gaps = 1/105 (0%)
Frame = -3
Query: 421 DTCGAKKIIIKDAAAPNYTSRKPSK*VAEIF*VKPGPLN*QTSKEHEQLEVMPLL-EKSM 245
D G K + ++ Y K K + + P N + +EVMPLL E+++
Sbjct: 89 DEAGLKSVTLEIEGRYAYGYLKSEKGTHRLVRISPFNANGKRQTSFAGVEVMPLLGEEAI 148
Query: 244 DTERLGPGN*FFNSREGGKGGQIVDKVEIAVQITHNQTGVTIH*T 110
+ SR GGKGGQ V+KVE AV+I H TG+ + T
Sbjct: 149 SLDIPDKDLDISTSRAGGKGGQNVNKVETAVRIVHLPTGLAVRCT 193
>ref|NP_563086.1| peptide chain release factor 2 [Clostridium perfringens]
gi|18145835|dbj|BAB81876.1| peptide chain release factor
2 [Clostridium perfringens str. 13]
Length = 323
Score = 47.0 bits (110), Expect = 2e-04
Identities = 34/111 (30%), Positives = 51/111 (45%), Gaps = 1/111 (0%)
Frame = -3
Query: 421 DTCGAKKIIIKDAAAPNYTSRKPSK*VAEIF*VKPGPLN*QTSKEHEQLEVMPLLEKSMD 242
D G K + +K Y K K + + + P N + +EV+P L D
Sbjct: 131 DEAGVKSVTLKVKGEFAYGYLKAEKGIHRLVRISPFNANGKRQTSFASVEVLPELTSDQD 190
Query: 241 TERLGPGN*FFNS-REGGKGGQIVDKVEIAVQITHNQTGVTIH*TSYFSSF 92
E + P + ++ R GG GGQ V+K E AV+ITH TG+ + + S F
Sbjct: 191 IE-INPVDLRIDTYRAGGAGGQHVNKTESAVRITHIPTGIVVQCQNERSQF 240
>ref|NP_781032.1| bacterial peptide chain release factor 2 [Clostridium tetani E88]
gi|28202524|gb|AAO34969.1| bacterial peptide chain
release factor 2 [Clostridium tetani E88]
Length = 325
Score = 47.0 bits (110), Expect = 2e-04
Identities = 32/101 (31%), Positives = 44/101 (42%)
Frame = -3
Query: 421 DTCGAKKIIIKDAAAPNYTSRKPSK*VAEIF*VKPGPLN*QTSKEHEQLEVMPLLEKSMD 242
D G K + +K Y K K V I + P N + LE++P L + D
Sbjct: 132 DEAGIKSVTLKITGDFAYGYLKAEKGVHRIVRISPYNANGKRQTSFASLELIPELTEDQD 191
Query: 241 TERLGPGN*FFNSREGGKGGQIVDKVEIAVQITHNQTGVTI 119
E + R G GGQ V+K E AV+ITH TG+ +
Sbjct: 192 IEIIDDDLKVDTYRASGAGGQHVNKTESAVRITHIPTGIVV 232
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 475,703,595
Number of Sequences: 1393205
Number of extensions: 9622628
Number of successful extensions: 18120
Number of sequences better than 10.0: 164
Number of HSP's better than 10.0 without gapping: 17455
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18114
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21997688174
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
