Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC016661A_C01 KMC016661A_c01
(568 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9ZTS1|SYM_ORYSA Probable methionyl-tRNA synthetase (Methioni... 145 3e-34
ref|NP_193114.1| methionyl-tRNA synthetase - like protein; prote... 144 8e-34
gb|AAH14051.1|AAH14051 Similar to small inducible cytokine subfa... 96 3e-19
ref|NP_004748.1| small inducible cytokine subfamily E, member 1;... 96 3e-19
pdb|1EUJ|A Chain A, A Novel Anti-Tumor Cytokine Contains A Rna-B... 96 3e-19
>sp|Q9ZTS1|SYM_ORYSA Probable methionyl-tRNA synthetase (Methionine--tRNA ligase)
(MetRS) gi|4091008|gb|AAC99620.1| methionyl-tRNA
synthetase [Oryza sativa]
Length = 804
Score = 145 bits (366), Expect = 3e-34
Identities = 70/95 (73%), Positives = 76/95 (79%)
Frame = -3
Query: 566 RGIKSHAMVLAASNDDHTKVELVESPSSAVVGERVTFPGFEGNPDELLNPKKKVWETLQV 387
RGIKSHAMVLAASN+DHTKVELVE P SA VGERVTF G+ G P+ LN K K WE L
Sbjct: 710 RGIKSHAMVLAASNEDHTKVELVEPPESAAVGERVTFAGYSGEPEASLNAKSKTWEKLSA 769
Query: 386 DLHSNEELVACYKNIPLTTSAGVCKVSSIRNGSIR 282
DLHSN ELVACYK++P TTSAGVCKV SI +G IR
Sbjct: 770 DLHSNGELVACYKDVPFTTSAGVCKVKSIASGEIR 804
>ref|NP_193114.1| methionyl-tRNA synthetase - like protein; protein id: At4g13780.1,
supported by cDNA: gi_20259314 [Arabidopsis thaliana]
gi|12230648|sp|Q9SVN5|SYM_ARATH Probable methionyl-tRNA
synthetase (Methionine--tRNA ligase) (MetRS)
gi|7437670|pir||T05247 methionine-tRNA ligase homolog
F18A5.170 - Arabidopsis thaliana
gi|4455307|emb|CAB36842.1| methionyl-tRNA
synthetase-like protein [Arabidopsis thaliana]
gi|7268082|emb|CAB78420.1| methionyl-tRNA
synthetase-like protein [Arabidopsis thaliana]
gi|17381106|gb|AAL36365.1| putative methionyl-tRNA
synthetase [Arabidopsis thaliana]
gi|20259315|gb|AAM14393.1| putative methionyl-tRNA
synthetase [Arabidopsis thaliana]
Length = 797
Score = 144 bits (363), Expect = 8e-34
Identities = 70/95 (73%), Positives = 81/95 (84%)
Frame = -3
Query: 566 RGIKSHAMVLAASNDDHTKVELVESPSSAVVGERVTFPGFEGNPDELLNPKKKVWETLQV 387
R I S AMVLAAS+ D +KVELVE P +A +GERVTFPGFEG PD++LNPKKKVWETL V
Sbjct: 703 RDIVSQAMVLAASSSDGSKVELVEPPKTANIGERVTFPGFEGEPDDVLNPKKKVWETLLV 762
Query: 386 DLHSNEELVACYKNIPLTTSAGVCKVSSIRNGSIR 282
DL++ E LVACYK++P TTSAGVCKVSSI NG+IR
Sbjct: 763 DLNTKENLVACYKDVPFTTSAGVCKVSSISNGTIR 797
>gb|AAH14051.1|AAH14051 Similar to small inducible cytokine subfamily E, member 1
(endothelial monocyte-activating) [Homo sapiens]
Length = 312
Score = 95.9 bits (237), Expect = 3e-19
Identities = 44/96 (45%), Positives = 65/96 (66%), Gaps = 1/96 (1%)
Frame = -3
Query: 566 RGIKSHAMVLAASNDDHTKVELVESPSSAVVGERVTFPGFEGNPDELLNPKKKVWETLQV 387
RG+ S AMV+ AS+ + K+E++ P+ +V G+R+TF F G PD+ LNPKKK+WE +Q
Sbjct: 219 RGVLSQAMVMCASSPE--KIEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQP 276
Query: 386 DLHSNEELVACYKNIPLTTSA-GVCKVSSIRNGSIR 282
DLH+N+E VA YK +P GVC+ ++ N I+
Sbjct: 277 DLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSGIK 312
>ref|NP_004748.1| small inducible cytokine subfamily E, member 1; endothelial
monocyte-activating polypeptide [Homo sapiens]
gi|18202490|sp|Q12904|MCA1_HUMAN Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] gi|498910|gb|AAA62202.1|
endothelial-monocyte activating polypeptide II
Length = 312
Score = 95.9 bits (237), Expect = 3e-19
Identities = 44/96 (45%), Positives = 65/96 (66%), Gaps = 1/96 (1%)
Frame = -3
Query: 566 RGIKSHAMVLAASNDDHTKVELVESPSSAVVGERVTFPGFEGNPDELLNPKKKVWETLQV 387
RG+ S AMV+ AS+ + K+E++ P+ +V G+R+TF F G PD+ LNPKKK+WE +Q
Sbjct: 219 RGVLSQAMVMCASSPE--KIEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQP 276
Query: 386 DLHSNEELVACYKNIPLTTSA-GVCKVSSIRNGSIR 282
DLH+N+E VA YK +P GVC+ ++ N I+
Sbjct: 277 DLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSGIK 312
>pdb|1EUJ|A Chain A, A Novel Anti-Tumor Cytokine Contains A Rna-Binding Motif
Present In Aminoacyl-Trna Synthetases
gi|10120517|pdb|1EUJ|B Chain B, A Novel Anti-Tumor
Cytokine Contains A Rna-Binding Motif Present In
Aminoacyl-Trna Synthetases gi|1619592|emb|CAA69993.1|
human EMAPII [synthetic construct]
Length = 166
Score = 95.9 bits (237), Expect = 3e-19
Identities = 44/96 (45%), Positives = 65/96 (66%), Gaps = 1/96 (1%)
Frame = -3
Query: 566 RGIKSHAMVLAASNDDHTKVELVESPSSAVVGERVTFPGFEGNPDELLNPKKKVWETLQV 387
RG+ S AMV+ AS+ + K+E++ P+ +V G+R+TF F G PD+ LNPKKK+WE +Q
Sbjct: 73 RGVLSQAMVMCASSPE--KIEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQP 130
Query: 386 DLHSNEELVACYKNIPLTTSA-GVCKVSSIRNGSIR 282
DLH+N+E VA YK +P GVC+ ++ N I+
Sbjct: 131 DLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSGIK 166
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 480,706,670
Number of Sequences: 1393205
Number of extensions: 10010649
Number of successful extensions: 27528
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 26063
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27468
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20669577624
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
