>KMC016507A_C01 KMC016507A_c01
caacaaaggaatccaagtttatgggtctccggtgcgtgtcaaacagaagtgtttcatata
atcaagacaacaccttaagTGCCAACAGAACACTAGATGGTTTATTCCAATTTTCTTTCT
TATTCACATCCTATTACTATCCTAATAACCAAATTACAAATCCTTCATCTTCTTTCCCAG
CATAAACAACTGGTTTCTATTTGAAAGAAATTAAAGCTTAATTTTTAATATTTAGCCTCT
TCCATATCTAAATGGTTCCTCCATTTATAGGAAGTCATTCAACCAAGGGATTTAAACAAC
CAACTTTTCCTCGTCCATTTCAGGGACGACAACATCTTCCCTCATTGAATGTGCATAATT
CTTTTCAGCTGTCGCCCCAGCCTGTGCAAATAGCTCAACCACCGCAGGCAGCATACCATA
GTACCTTTTAAGTTCATGTACCAAAGGCTTCCCGGATTGCTGATCTCCAATGTGGTAGAC
GTATACAGGAGGTACCAAATCATTAATTGTAGCCTCCTGCCATGCATGCTGnCCATCCCT
CATCTGGTGTTTGAAAGCTTGACAATTTCGTACTCTGTG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC016507A_C01 KMC016507A_c01
         (579 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_568945.1| putative protein; protein id: At5g61930.1, supp...   110  2e-23
dbj|BAB08879.1| gb|AAD38255.1~gene_id:K22G18.5~similar to unknow...   110  2e-23
dbj|BAC15509.1| hypothetical protein~similar to Arabidopsis thal...   110  2e-23
ref|NP_200601.2| putative protein; protein id: At5g57930.1, supp...   101  6e-21
dbj|BAB08863.1| gb|AAD38255.1~gene_id:MTI20.19~similar to unknow...   101  6e-21

>ref|NP_568945.1| putative protein; protein id: At5g61930.1, supported by cDNA:
           gi_13899116 [Arabidopsis thaliana]
           gi|13899117|gb|AAK48980.1|AF370553_1 Unknown protein
           [Arabidopsis thaliana] gi|25083634|gb|AAN72097.1|
           Unknown protein [Arabidopsis thaliana]
          Length = 340

 Score =  110 bits (274), Expect = 2e-23
 Identities = 52/94 (55%), Positives = 63/94 (66%)
 Frame = -1

Query: 579 HRVRNCQAFKHQMRDGQHAWQEATINDLVPPVYVYHIGDQQSGKPLVHELKRYYGMLPAV 400
           H+VR C+A KHQMRDG HAWQEATI+D+V P YV+H+ D   G  L + LKR+YG  PAV
Sbjct: 246 HKVRMCKATKHQMRDGMHAWQEATIDDVVGPTYVWHVRDPTDGSVLDNSLKRFYGKAPAV 305

Query: 399 VELFAQAGATAEKNYAHSMREDVVVPEMDEEKLV 298
           +E+  Q GA     Y   MR DVV P+ DE  LV
Sbjct: 306 IEMCVQGGAPVPDQYNSMMRLDVVYPQRDEVDLV 339

 Score = 40.0 bits (92), Expect = 0.021
 Identities = 20/73 (27%), Positives = 34/73 (46%)
 Frame = -1

Query: 579 HRVRNCQAFKHQMRDGQHAWQEATINDLVPPVYVYHIGDQQSGKPLVHELKRYYGMLPAV 400
           H +R C       R   H W+   ++D+V     +H+ D+     ++H+ +     + AV
Sbjct: 79  HEIRTCTGPGSGSRSATHVWKRGRVSDVVLFPKCFHLYDRAVKPRVIHDERFTVPKISAV 138

Query: 399 VELFAQAGATAEK 361
           +EL  QAG   EK
Sbjct: 139 LELCIQAGVDLEK 151

>dbj|BAB08879.1| gb|AAD38255.1~gene_id:K22G18.5~similar to unknown protein
           [Arabidopsis thaliana]
          Length = 402

 Score =  110 bits (274), Expect = 2e-23
 Identities = 52/94 (55%), Positives = 63/94 (66%)
 Frame = -1

Query: 579 HRVRNCQAFKHQMRDGQHAWQEATINDLVPPVYVYHIGDQQSGKPLVHELKRYYGMLPAV 400
           H+VR C+A KHQMRDG HAWQEATI+D+V P YV+H+ D   G  L + LKR+YG  PAV
Sbjct: 308 HKVRMCKATKHQMRDGMHAWQEATIDDVVGPTYVWHVRDPTDGSVLDNSLKRFYGKAPAV 367

Query: 399 VELFAQAGATAEKNYAHSMREDVVVPEMDEEKLV 298
           +E+  Q GA     Y   MR DVV P+ DE  LV
Sbjct: 368 IEMCVQGGAPVPDQYNSMMRLDVVYPQRDEVDLV 401

 Score = 40.0 bits (92), Expect = 0.021
 Identities = 20/73 (27%), Positives = 34/73 (46%)
 Frame = -1

Query: 579 HRVRNCQAFKHQMRDGQHAWQEATINDLVPPVYVYHIGDQQSGKPLVHELKRYYGMLPAV 400
           H +R C       R   H W+   ++D+V     +H+ D+     ++H+ +     + AV
Sbjct: 141 HEIRTCTGPGSGSRSATHVWKRGRVSDVVLFPKCFHLYDRAVKPRVIHDERFTVPKISAV 200

Query: 399 VELFAQAGATAEK 361
           +EL  QAG   EK
Sbjct: 201 LELCIQAGVDLEK 213

>dbj|BAC15509.1| hypothetical protein~similar to Arabidopsis thaliana chromosome
           5K22G18.5 [Oryza sativa (japonica cultivar-group)]
          Length = 517

 Score =  110 bits (274), Expect = 2e-23
 Identities = 56/99 (56%), Positives = 64/99 (64%), Gaps = 5/99 (5%)
 Frame = -1

Query: 579 HRVRNCQAFKHQMRDGQHAWQEATINDLVPPVYVYHI-----GDQQSGKPLVHELKRYYG 415
           H+VR C+A KHQ RDGQHAWQEAT++DLV P YV+H+     G      PL +ELKRYYG
Sbjct: 418 HKVRMCKASKHQQRDGQHAWQEATVDDLVRPNYVWHVPATGHGGDGGAPPLANELKRYYG 477

Query: 414 MLPAVVELFAQAGATAEKNYAHSMREDVVVPEMDEEKLV 298
             PAVVEL  +AGA     Y   MR DVV P  DE  LV
Sbjct: 478 KAPAVVELCVRAGAPVPAQYRSMMRLDVVPPARDEHDLV 516

 Score = 50.1 bits (118), Expect = 2e-05
 Identities = 27/74 (36%), Positives = 39/74 (52%), Gaps = 1/74 (1%)
 Frame = -1

Query: 579 HRVRNCQAFKHQMRDGQHAWQEATINDLVPPVYVYHIGDQQSGKPLVHELKRY-YGMLPA 403
           H +R C+      R+  H W+  T  D+V   Y YH+ D+  GKP V   ++Y    LPA
Sbjct: 249 HEIRTCEGPGSAARNALHVWRPGTARDVVGFPYCYHLFDRV-GKPRVSHKEKYDVPRLPA 307

Query: 402 VVELFAQAGATAEK 361
           ++EL  QAG   E+
Sbjct: 308 ILELCIQAGVDVER 321

>ref|NP_200601.2| putative protein; protein id: At5g57930.1, supported by cDNA:
           gi_17065249 [Arabidopsis thaliana]
           gi|17065250|gb|AAL32779.1| Unknown protein [Arabidopsis
           thaliana] gi|21387175|gb|AAM47991.1| unknown protein
           [Arabidopsis thaliana]
          Length = 440

 Score =  101 bits (252), Expect = 6e-21
 Identities = 46/95 (48%), Positives = 66/95 (69%)
 Frame = -1

Query: 579 HRVRNCQAFKHQMRDGQHAWQEATINDLVPPVYVYHIGDQQSGKPLVHELKRYYGMLPAV 400
           H+ +NC AFKHQ R+GQH WQ A ++DL+PP YV+H+ D  +G P+  EL+ +YG  PAV
Sbjct: 346 HKAQNCGAFKHQQRNGQHGWQSAVLDDLIPPRYVWHVPD-VNGPPMQRELRSFYGQAPAV 404

Query: 399 VELFAQAGATAEKNYAHSMREDVVVPEMDEEKLVV 295
           VE+ AQAGA   ++Y  +MR +V +P   +E  +V
Sbjct: 405 VEICAQAGAVVPEHYRATMRLEVGIPSSVKEAEMV 439

 Score = 47.8 bits (112), Expect = 1e-04
 Identities = 25/84 (29%), Positives = 42/84 (49%)
 Frame = -1

Query: 579 HRVRNCQAFKHQMRDGQHAWQEATINDLVPPVYVYHIGDQQSGKPLVHELKRYYGMLPAV 400
           H  ++C+      R G H W  + I D++ P+  YH+ D + GK + H+ +     +PAV
Sbjct: 176 HPFKSCKGPNTSQRKGLHEWTNSVIEDVIVPLEAYHLFD-RLGKRIRHDERFSIPRVPAV 234

Query: 399 VELFAQAGATAEKNYAHSMREDVV 328
           VEL  Q G    +  A   R+ ++
Sbjct: 235 VELCIQGGVEIPEFPAKRRRKPII 258

>dbj|BAB08863.1| gb|AAD38255.1~gene_id:MTI20.19~similar to unknown protein
           [Arabidopsis thaliana]
          Length = 405

 Score =  101 bits (252), Expect = 6e-21
 Identities = 46/95 (48%), Positives = 66/95 (69%)
 Frame = -1

Query: 579 HRVRNCQAFKHQMRDGQHAWQEATINDLVPPVYVYHIGDQQSGKPLVHELKRYYGMLPAV 400
           H+ +NC AFKHQ R+GQH WQ A ++DL+PP YV+H+ D  +G P+  EL+ +YG  PAV
Sbjct: 311 HKAQNCGAFKHQQRNGQHGWQSAVLDDLIPPRYVWHVPD-VNGPPMQRELRSFYGQAPAV 369

Query: 399 VELFAQAGATAEKNYAHSMREDVVVPEMDEEKLVV 295
           VE+ AQAGA   ++Y  +MR +V +P   +E  +V
Sbjct: 370 VEICAQAGAVVPEHYRATMRLEVGIPSSVKEAEMV 404

 Score = 47.8 bits (112), Expect = 1e-04
 Identities = 25/84 (29%), Positives = 42/84 (49%)
 Frame = -1

Query: 579 HRVRNCQAFKHQMRDGQHAWQEATINDLVPPVYVYHIGDQQSGKPLVHELKRYYGMLPAV 400
           H  ++C+      R G H W  + I D++ P+  YH+ D + GK + H+ +     +PAV
Sbjct: 141 HPFKSCKGPNTSQRKGLHEWTNSVIEDVIVPLEAYHLFD-RLGKRIRHDERFSIPRVPAV 199

Query: 399 VELFAQAGATAEKNYAHSMREDVV 328
           VEL  Q G    +  A   R+ ++
Sbjct: 200 VELCIQGGVEIPEFPAKRRRKPII 223

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 518,545,364
Number of Sequences: 1393205
Number of extensions: 11672464
Number of successful extensions: 24193
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 23419
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24165
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21426319650
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPDL036g04_f BP054289 1 488 2 SPDL053f01_f BP055358 44 580

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